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  4. Human ADAM DEC1(ADAMDEC1) ELISA kit

Human ADAM DEC1(ADAMDEC1) ELISA kit

  • 中文名称：
    人ADAM DEC1(ADAMDEC1)酶联免疫试剂盒
  • 货号：
    CSB-EL001297HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

        人ADAMDEC1 ELISA试剂盒是为了定量测量血清、血浆和组织匀浆中的人类ADAMDEC1蛋白而设计的。j9九游会登录入口首页生物夹心ELISA试剂盒，其检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。

  • 别名：
    A disintegrin and metalloproteinase domain like protein decysin 1 ELISA Kit; A disintegrin and metalloproteinase domain-like protein decysin-1 ELISA Kit; ADAM DEC1 ELISA Kit; ADAM like decysin 1 ELISA Kit; ADAM like protein decysin 1 ELISA Kit; ADAM-like protein decysin-1 ELISA Kit; ADAMDEC 1 ELISA Kit; Adamdec1 ELISA Kit; ADEC1_HUMAN ELISA Kit; Decysin ELISA Kit; Disintegrin protease ELISA Kit; M12.219 ELISA Kit
  • 缩写：
    ADAMDEC1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMDEC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 87-96    
    1:2 Average % 96    
    Range % 90-101    
    1:4 Average % 94    
    Range % 89-99    
    1:8 Average % 97    
    Range % 92-103    
  • 回收率：
    The recovery of human ADAMDEC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 99-108    
    EDTA plasma (n=4) 94 89-98    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.224 2.364 2.294 2.141    
    2000 1.732 1.667 1.700 1.547    
    1000 1.127 1.071 1.099 0.946    
    500 0.702 0.713 0.708 0.555    
    250 0.431 0.442 0.437 0.284    
    125 0.314 0.321 0.318 0.165    
    62.5 0.246 0.252 0.249 0.096    
    0 0.152 0.154 0.153 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    May play an important role in the control of the immune response and during pregnancy.
  • 基因功能参考文献：
    1. Data suggest that activated platelets release ADAMDEC1, which hydrolyzes pro-EGF (epidermal growth factor) to soluble, active HMW-EGF; proteolytic cleavage of pro-EGF first occurs at the C-terminal arginyl residue of the EGF domain; proteolysis is the regulated, rate-limiting step in generating soluble EGF from activated platelets. PMID: 28455445
    2. We hypothesize that these unique features of ADAMDEC1 may have evolved to escape from inhibition by endogenous metalloprotease inhibitors PMID: 25564618
    3. ADAMDEC1 functions as an active metalloprotease. PMID: 23754285
    4. molecular evolution by duplication in a metalloprotease gene cluster on chromosome 8p12 PMID: 12037602
    5. The ADAMDEC 1 haplotype may indicate an underlying mechanism for high FVIII levels in venous thromboembolism. PMID: 18449420
    6. Gene expression profiling performed on tissues obtained from pulmonary sarcoidosis patients identified ADAMDEC1 as a potential pathogenic mediator of lung damage and/or remodeling and may serve as a marker for this disease. PMID: 19218196

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in mon
  • 数据库链接：

    HGNC: 16299

    OMIM: 606393

    KEGG: hsa:27299

    STRING: 9606.ENSP00000256412

    UniGene: Hs.521459