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Goat growth hormone,GH ELISA KIT

  • 中文名称：
    山羊生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E13275G
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Goat GH1 ELISA Kit was designed for the quantitative measurement of Goat GH1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-100 ng/mL and the sensitivity is 6.25 ng/mL.

  • 别名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 ng/mL-100 ng/mL
  • 灵敏度：
    6.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 95  
    Range % 91-99  
    1:8 Average % 92  
    Range % 88-97  
  • 回收率：
    The recovery of goat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 94 89-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.202 0.200 0.201    
    50 0.396 0.365 0.381    
    25 0.631 0.622 0.627    
    12.5 1.142 1.154 1.148    
    6.25 2.359 2.326 2.343    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.
For example:#CSB-E13275G,Goat growth hormone,GH ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
The standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶点详情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能参考文献：
    1. genetic variation and milk production and composition PMID: 23611522
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    KEGG: chx:100861230

    UniGene: Chi.38639