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  4. Goat Thyroxine(T4) ELISA Kit

Goat Thyroxine(T4) ELISA Kit

  • 中文名称：
    山羊甲状腺素(T4)酶联免疫试剂盒
  • 货号：
    CSB-E13384G
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        甲状腺素T4是由甲状腺分泌的一种激素，可以调节身体内的代谢率、体温、心率、血压等生理功能。T4可以在体内转化为另一种甲状腺激素T3，而T3则更为活跃，具有更强的生物活性。T4水平的异常会导致甲状腺功能亢进或甲状腺功能减退等疾病。此外，在妊娠期间，T4水平的变化还可能对胎儿的神经发育和生长发育产生影响。
        j9九游会登录入口首页生物所提供的Goat Thyroxine(T4) ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测山羊血清、血浆、组织匀浆样本中的T4，其灵敏度为20 ng/mL，检测范围为20 ng/mL-320 ng/mL。
     
  • 缩写：
    T4
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    20 ng/mL-320 ng/mL
  • 灵敏度：
    20 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 86-101    
    1:2 Average % 86    
    Range % 81-98    
    1:4 Average % 95    
    Range % 90-100    
    1:8 Average % 95    
    Range % 90-100    
  • 回收率：
    The recovery of goat T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 92-110    
    EDTA plasma (n=4) 95 90-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average 　    
    320 0.163 0.169 0.166 　    
    160 0.275 0.281 0.278 　    
    80 0.493 0.502 0.498 　    
    40 1.015 1.029 1.022 　    
    20 2.049 2.068 2.059 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13384G,Goat Thyroxine(T4) ELISA Kit *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
The standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶点详情

  • 最新研究进展：
    T4是一种重要的代谢激素，最新研究表明T4与身体免疫系统密切相关。研究人员正在探索T4对免疫细胞功能的影响，以及利用T4来治疗免疫系统相关疾病的可能性。