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  4. Mouse thyroxine,T4 ELISA Kit

Mouse thyroxine,T4 ELISA Kit

  • 中文名称：
    小鼠甲状腺素(T4)酶联免疫试剂盒
  • 货号：
    CSB-E05083m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        T4，全称为甲状腺素，是一种由甲状腺分泌的激素，它在机体内起到调节新陈代谢的作用，调控体温、能量消耗和心脏、神经系统的正常功能。T4进入血液后会与血浆中的蛋白质结合，主要是结合甲状腺结合球蛋白，使其稳定并运输至身体需要的地方。当身体需要时，T4会被转化为T3，这是一种更加活跃的形式，可以进入细胞并发挥作用。T4的水平可以通过血液测试来检测，这有助于诊断甲状腺疾病和其他相关疾病。
        j9九游会登录入口首页生物所提供的Mouse thyroxine,T4 ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的T4，其灵敏度为20 ng/mL，检测范围为20 ng/mL-320 ng/mL。
     
  • 缩写：
    T4
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    20 ng/mL-320 ng/mL
  • 灵敏度：
    20 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 标准曲线：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本试剂盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody.
      • Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-conjugated T4 1 x 6 ml
      • HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶点详情

  • 最新研究进展：
    近期研究发现，T4蛋白在维持人类健康和免疫系统中发挥着重要的作用，特别是对于甲状腺激素代谢的平衡具有重要作用。