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Goat Immunoglobulin A(IgA) ELISA Kit

  • 中文名称:
    山羊免疫球蛋白A(IgA)酶联免疫试剂盒
  • 货号:
    CSB-E13135G
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Goat IgA ELISA Kit was designed for the quantitative measurement of Goat IgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 29 ng/mL-7500 ng/mL and the sensitivity is 29 ng/mL.

  • 缩写:
    IgA
  • 种属:
    Capra hircus (Goat)
  • 样本类型:
    serum, plasma
  • 检测范围:
    29 ng/mL-7500 ng/mL
  • 灵敏度:
    29 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5000 Average % 94  
    Range % 90-103  
    1:10000 Average % 96  
    Range % 92-100  
    1:20000 Average % 90  
    Range % 85-97  
    1:40000 Average % 92  
    Range % 85-103  
  • 回收率:
    The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-100  
    EDTA plasma (n=4) 95 91-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    7500 0.289 0.312 0.301    
    1875 0.558 0.542 0.550    
    468 0.912 0.921 0.917    
    117 1.612 1.543 1.578    
    29 1.853 1.934 1.894    
    0 2.406 2.398 2.402    
                 
  • 数据处理:
  • 货期:
    3-5 working days

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