Your Good Partner in Biology Research

Bovine Immunoglobulin M,IgM ELISA Kit

  • 中文名称:
    牛免疫球蛋白M(IgM)酶联免疫试剂盒
  • 货号:
    CSB-E12017B
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
        IgM是一种多聚体免疫球蛋白,通常作为初次感染后产生的第一个免疫球蛋白。IgM能够识别并结合细菌、病毒和其他外来物质,从而激活免疫系统并参与免疫反应。IgM的水平可以用于诊断某些疾病,如乙肝、风疹等。
        j9九游会登录入口首页生物所提供的Bovine Immunoglobulin M,IgM ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、细胞培养上清、组织匀浆样本中的IgM,其灵敏度为0.2 μg/mL,检测范围为0.39 μg/mL-25 μg/mL。
     
  • 缩写:
    IgM
  • 种属:
    Bos taurus (Bovine)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    0.39 μg/mL-25 μg/mL
  • 灵敏度:
    0.2 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-99  
    1:200 Average % 97  
    Range % 92-103  
    1:400 Average % 85  
    Range % 81-87  
    1:800 Average % 89  
    Range % 83-95  
  • 回收率:
    The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 97 92-102  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    25 0.059 0.056 0.058    
    12.5 0.091 0.099 0.095    
    6.25 0.151 0.152 0.152    
    3.12 0.312 0.292 0.302    
    1.56 0.608 0.660 0.634    
    0.78 0.824 0.801 0.813    
    0.39 1.150 1.192 1.171    
    0 1.552 1.508 1.530    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
    IgM是一种免疫球蛋白,被认为是先天免疫系统的一个关键组成部分。最近的研究表明,IgM在多种疾病的预防和治疗中具有重要作用。例如,在新冠疫情期间,研究人员使用IgM抗体来诊断和治疗感染。此外,IgM还被用于治疗其他疾病,如类风湿关节炎和多发性硬化症。