Acvrl1

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ACVRL1 at 2μg/mL can bind Anti-ACVRL1 recombinant antibody(CSB-RA001262MA1HU)，the EC₅₀ is 2.417-2.971 ng/mL.

Positive WB detected in: Daudi whole cell lysate, U87 whole cell lysate
All lanes: ACVRL1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 65 kDa

IHC image of CSB-RA555022A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.