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  4. Recombinant Human Neuroendocrine convertase 1 (PCSK1)

Recombinant Human Neuroendocrine convertase 1 (PCSK1)

  • 货号：
    CSB-YP017640HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017640HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017640HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017640HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017640HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PCSK1
  • Uniprot No.：
  • 别名：
    BDP; NEC 1; NEC1; NEC1_HUMAN; Neuroendocrine convertase 1; PC1; PC3; PCSK1; Prohormone convertase 1; Prohormone convertase 1/3; Prohormone convertase 3; Proprotein convertase 1; SPC3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    111-753
  • 氨基酸序列
    SALRDSALNL FNDPMWNQQW YLQDTRMTAA LPKLDLHVIP VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV DLADPRTWRS VPEKKECVVK DNDFEPRALK ANGEVIIEIP TRACEGQENA IKSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN WDFMSVHTWG ENPIGTWTLR ITDMSGRIQN EGRIVNWKLI LHGTSSQPEH MKQPRVYTSY NTVQNDRRGV EKMVDPGEEQ PTQENPKENT LVSKSPSSSS VGGRRDELEE GAPSQAMLRL LQSAFSKNSP PKQSPKKSPS AKLNIPYENF YEALEKLNKP SQLKDSEDSL YNDYVDVFYN TKPYKHRDDR LLQALVDILN EEN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
  • 基因功能参考文献：
    1. we found eight known variants and two novel heterozygous variants (c.1095 + 1G > A and p.S24C), and identified and functionally characterized two rare novel PCSK1 variants of which c.1095 + 1G > A caused complete loss of protein function. In addition to confirming rs6232 and rs6234 in PCSK1 as polygenic risk variants for childhood obesity, we describe an association of rs725522 with insulin metabolism. PMID: 28271036
    2. investigation of the pathogenesis of obesity in the PC1/3-N222D mouse model and whether this molecular mechanism also applies to common and rare human PCSK1 mutations PMID: 26786350
    3. PCSK1 deficiency plays a role in human endocrinopathies, obesity, gastrointestinal disorders. PMID: 27187081
    4. findings suggest that the major neuroendocrine features of Prader-Willi syndrome are due to PC1 deficiency PMID: 27941249
    5. PCSK1 mutations are associated with Obesity. PMID: 27288825
    6. PCSK1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    7. these common variants in PCSK1 and POMC were not the major cause of obesity in the Thai subjects sampled. However, variants in PCSK1 did affect cholesterol level, LDL-C level, and waist circumference. PMID: 26345846
    8. conclude that the N309K enzyme is able to cleave its own propeptide but is catalytically inert against in trans substrates, and that this variant accounts for the enteric and systemic endocrinopathies seen in the consanguineous kindred studied PMID: 25272002
    9. we demonstrate evidence that common gene variation in PCSK1 contributes to BMI variation and susceptibility to common obesity in the largest known meta-analysis published to date in genetic epidemiology. PMID: 25784503
    10. Data show that prohormone convertase 1/3 (PC1/3) endoplasmic reticulum-retained mutants induce endoplasmic reticulum stress. PMID: 26207343
    11. Mechanism of Fine-tuning pH Sensors in Proprotein Convertases: IDENTIFICATION OF A pH-SENSING HISTIDINE PAIR IN THE PROPEPTIDE OF PROPROTEIN CONVERTASE 1/3. PMID: 26229104
    12. rs6232 associated with body mass index PMID: 25031086
    13. We showed for the first time that a nonsense mutation in PCSK1 was likely to cause dominantly inherited human obesity, due to the inhibiting properties of the propeptide fragment encoded by the null allele PMID: 24890885
    14. Epistases between single nucleotide polymorphisms within proprotein convertase subtilisin/kexin type 1(PCSK1) and dopamine beta-hydroxylase(DBH) genes are significantly associated with susceptibility or resistance to premature ovarian failure PMID: 24618767
    15. FTO-rs9939609, TMEM18-rs6548238 and PCSK1-rs6234 polymorphisms are significantly associated with body mass index in a southern Chinese population. PMID: 25189249
    16. SNPs rs6232, rs6234, and rs6235 associated with obesity in Caucasians [review] PMID: 25355447
    17. Common genetic variants in PCSK1 are associated with coronary artery disease in Chinese patients with type 2 diabetes. PMID: 24489861
    18. PC1/3(S357G) exhibited a lower calcium dependence; a higher pH optimum. PMID: 24932808
    19. PCSK1 is overexpressed in fibrolamellar hepatocellular carcinoma. PMID: 24443104
    20. Thais carrying SNPs rs6234-5 are at increased risk of obesity, and the risk of severe obesity increases when carrying both rs6234-5 and rs3811951, but not with rs271939. Patients with genetic variations at rs3811951 are at risk of having low HDL-C levels. PMID: 24964673
    21. PC1/3 overexpression induces morphological and phenotypic epithelial-mesenchymal transition changes of airway epithelial cells involved in the pathogenesis of nasal polyps PMID: 23314902
    22. Data show the synthetic effect of SNPs on the indices of adiposity and risk of obesity in Chinese girls, but failed to replicate the effect of five separate variants of SEC16B rs10913469, SH2B1 rs4788102, PCSK1 rs6235, KCTD15 rs29941 and BAT2 rs2844479. PMID: 23121087
    23. the development of central diabetes insipidus in this proband suggests that PCSK1 deficiency can be associated with impaired osmoregulation. PMID: 23800642
    24. Common PCSK1 variants (notably rs6232 and rs6235) contribute modestly to obesity in multi-ethnic American population. PMID: 23451278
    25. study indicates that the PCSK1 rs6235 SNP may contribute to the risk of overweight in men and predict obesity-related metabolic traits such as waist circumference and diastolic blood pressure in Taiwanese subjects PMID: 24140494
    26. The data of this study suggest that defects in the processing of hypothalamic neuropeptides in huntington disease may partially arise from decreased PC1/3 and PC2 expressions. PMID: 24226266
    27. novel R80Q (rs1799904) variant both exhibits adverse effects on PC1/3 activity and is prevalent in the population PMID: 23383060
    28. PCSK1 SNP rs6235 was associated with essential hypertension and blood pressure in the Han Chinese population PMID: 22592666
    29. PCSK1 rs6232 is associated with childhood and adult class III obesity in the Mexican population PMID: 22737226
    30. The potential variations in the exons and exon/intron junctions of caprine PC1 gene, were studied. PMID: 22552345
    31. Our data suggest that SNPs in or near the PCSK1 locus contribute to obesity risk in the Greek population. PMID: 21720444
    32. PCSK1 rs6232 G-allele and rs6235 C-allele have an effect on body composition which may be modified by sex PMID: 21935364
    33. an increased risk of obesity in heterozygous carriers of mutations in the PCSK1 gene. PMID: 22210313
    34. triple-variant prohormone convertase 1 underwent significantly more proteolytic processing at the N- and C-termini than the double-variant isoforms PMID: 22000902
    35. common PCSK1 genetic variants are associated with obesity in the Chinese population PMID: 19875984
    36. The rs6234 G-allele showed a significant association with increased risk of combined phenotype of obesity and overweight in Chinese. PMID: 20498726
    37. Studies of protease SPC3 (PC1/3) suggest that C-domain sequences contribute to cleavage site selection for proinsulin PMID: 20106974
    38. The presence of PC and GOAT in the cells, as well as n-octanoic acid in the culture medium, was necessary to produce n-octanoyl ghrelin. PMID: 19628676
    39. prominently expressed in PTHrP-expressing human cancer cell lines originating from tumors of the breast, lung, prostate, as well as lymphoma PMID: 11720250
    40. Polymorphism associated with insulin resistance affects insulin snssitivity by interacting with PPARgamma2. PMID: 14574455
    41. PC1 is present in the human cortex to fulfill its role in proteolytic processing of neuropeptide precursors; no significant change in PC1 levels is observed in Alzheimer patients. PMID: 14614908
    42. Data suggest that proprotein convertase 1, presumably in the enteroendocrine cells, is essential for the normal absorptive function of the human small intestine. PMID: 14617756
    43. results have implications for the mechanism of granule sorting of PC3 as well as for the topology of PC2 and carboxypeptidase E, which have been reported to span the lipid membrane by homologous charged sequences PMID: 15807527
    44. PC1 and PC2 in are abnormally expressed and processed in human colorectal liver metastases PMID: 16293189
    45. PC1 has a role in obesity, hyperphagia and increased metabolic efficiency PMID: 16644867
    46. A novel missense mutation Ser307Leu in PC 1/3 linked to hyperphagia and obesity. PMID: 17595246
    47. defective PC1/3 expression may lead to preferential production of unprocessed, biologically inactive ACTH variants in sient corticotroph adenoma. PMID: 17917309
    48. PC1/3 governs the endocrine and PC2 the neuronal processing of proCCK, whereas PC5/6 contributes only to a modest endocrine synthesis of CCK-22. PMID: 18096669
    49. Suppression of Pdcd4 resulted in an increased release of CgA and Sg II and was accompanied by an up-regulation of intracellular PC1. PMID: 18549351
    50. The nonsynonymous variants of PCSK1 rs6232, encoding N221D, and rs6234-rs6235, encoding the Q665E-S690T pair, were consistently associated with obesity in adults and children PMID: 18604207

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  • 相关疾病：
    Proprotein convertase 1 deficiency (PC1 deficiency)
  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules.
  • 蛋白家族：
    Peptidase S8 family, Furin subfamily
  • 数据库链接：

    HGNC: 8743

    OMIM: 162150

    KEGG: hsa:5122

    STRING: 9606.ENSP00000308024

    UniGene: Hs.78977