Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat bone alkaline phosphatase,BALP ELISA Kit

Rat bone alkaline phosphatase,BALP ELISA Kit

  • 中文名称：
    大鼠骨碱性磷酸酶(BALP)酶联免疫试剂盒
  • 货号：
    CSB-E11865r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat BALP ELISA Kit was designed for the quantitative measurement of Rat BALP protein in serum, tissue homogenates, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 U/L-1000 U/L and the sensitivity is 3.9 U/L .

  • 别名：
    Alpl ELISA Kit; Alkaline phosphatase ELISA Kit; tissue-nonspecific isozyme ELISA Kit; AP-TNAP ELISA Kit; TNSALP ELISA Kit; EC 3.1.3.1 ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit
  • 缩写：
    BALP
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, tissue homogenates, plasma
  • 检测范围：
    15.6 U/L-1000 U/L
  • 灵敏度：
    3.9 U/L
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %83-96
    1:2Average %96
    Range %92-105
    1:4Average %99
    Range %96-105
    1:8Average %95
    Range %90-104
  • 回收率：
    The recovery of rat BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9388-99
    EDTA plasma (n=4)9592-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    U/LOD1OD2AverageCorrected
    10002.561 2.375 2.468 2.301
    5001.854 1.886 1.870 1.703
    2501.272 1.234 1.253 1.086
    1250.805 0.817 0.811 0.644
    62.50.524 0.554 0.539 0.372
    31.20.362 0.353 0.358 0.191
    15.60.263 0.258 0.261 0.094
    00.170 0.163 0.167 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
  • 基因功能参考文献：
    1. he rats body weight were decreased, the cartilage histological structure were disrupted, the cartilage and serum contents of Al and the serum level of C-telopeptide of type II collagen were all increased, the serum level of type II collagen (Col II) and alkaline phosphatase (ALP), and the mRNA expressions of TGF-beta1, BMP-2, ALP and Col II were all decreased in the AlCl3-treated groups compared with those in control g... PMID: 27885436
    2. Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress. PMID: 27590577
    3. TNAP contributes to the hypertrophic effect of phenylephrine, as well as its ability to produce cardiomyocyte calcification. These responses are minimized by CD73-dependent endogenously produced adenosine. PMID: 24894822
    4. TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration. PMID: 25411053
    5. Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFalpha, IL-6 and iNOS. PMID: 24888842
    6. BMP-2 and ALP gene expression is induced by a BMP-2 gene-fibronectin-apatite composite layer PMID: 21636885
    7. The expression of ENPP1, TNAP and ANK proteins could be detected in each generation of endplate chondrocytes. With the continuing passage of cells, the level of protein expression declined gradually. PMID: 21418901
    8. Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615
    9. Forty SNPs in ALPL and 14 SNPs in ENPP1 genetic loci as well as pairwise haplotypes were tested for association with bone strength related traits. PMID: 19931660
    10. Characterization of rat Alpl isoforms by hplc and agar gel electrophoresis are reported. PMID: 19084045
    11. ATP-mediated DBS, forming a negative feedback loop. The duodenal epithelial brush border IAP-P2Y-HCO(3-) surface microclimate pH regulatory system effectively protects the mucosa from acid injury. PMID: 19451200

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Extracellular vesicle membrane; Lipid-anchor, GPI-anchor. Mitochondrion membrane; Lipid-anchor, GPI-anchor. Mitochondrion intermembrane space.
  • 蛋白家族：
    Alkaline phosphatase family
  • 数据库链接：