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Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名称：
    大鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
  • 货号：
    CSB-E10786r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Aβ1-42 ELISA Kit was designed for the quantitative measurement of Rat Aβ1-42 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 pg/mL-60 pg/mL and the sensitivity is 0.225 pg/mL .

  • 别名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 缩写：
    Aβ1-42
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.9 pg/mL-60 pg/mL
  • 灵敏度：
    0.225 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %83-90
    1:2Average %94
    Range %90-98
    1:4Average %101
    Range %97-104
    1:8Average %96
    Range %93-99
  • 回收率：
    The recovery of rat Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)10095-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    602.281 2.274 2.278 2.136
    301.599 1.576 1.588 1.446
    150.967 0.924 0.946 0.804
    7.50.538 0.528 0.533 0.391
    3.750.395 0.371 0.383 0.241
    1.80.321 0.312 0.317 0.175
    0.90.235 0.250 0.243 0.101
    00.141 0.143 0.142 　
  • 数据处理：
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

我们的实验室打算购买β淀粉样蛋白1-42，Aβ1-42 ELISA试剂盒，货号是CSB-E10786r。但是，我们使用的是含有SDS的RIPA缓冲液来裂解组织，同时我们也用相同的RIPA缓冲液和1x PBS稀释了样品。我想知道这样做是否会影响试剂盒的效果

A:
对于ELISA检测，一般不建议使用含有SDS的缓冲液处理样品。这是因为SDS可以破坏分子与分子间的氢键，即破坏蛋白质的二级和三级结构，并可能影响ELISA的检测。由于您的样品已经被处理过，我们只能建议您先使用处理后的样品进行梯度预实验。我们以前没有这样做过，所以我们只能建议你试一试。