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  4. Rat Interleukin 10,IL-10 ELISA KIT

Rat Interleukin 10,IL-10 ELISA KIT

  • 中文名称：
    大鼠白介素10(IL-10)酶联免疫试剂盒
  • 货号：
    CSB-E04595r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL-10是一种抗炎细胞因子，由多种细胞产生，如单核细胞、B细胞和T细胞。IL-10的作用包括抑制炎症反应、降低细胞因子和白细胞的活性。它还参与调节细胞生长和增殖。
        j9九游会登录入口首页生物所提供的Rat Interleukin 10,IL-10 ELISA KIT属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆样本中的IL10，其灵敏度为0.78 pg/mL，检测范围为3.12 pg/mL-200 pg/mL。
     
  • 别名：
    Il10 ELISA Kit; Il-10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates.
  • 检测范围：
    3.12 pg/ml-200 pg/ml.
  • 灵敏度：
    0.78 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: The levels of cytokines in the different groups (n = 9). The production of IL-10 in supernatant of cultured PMNs was detected by ELISA. PMNs were cultured with NPS-2143 (100 nM), cinacalcet (100 nM), and PDTC (100 nM) for 12 h after stimulated by fMLP (10 nM) for 2 min. #P < 0 05 versus control group, ∗P < 0 05 versus fMLP group, and △P < 0 01 versus fMLP + CIN group.
    PMID: 29081886
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: HO‐1 overexpression increases the production of paracrine factors from MSCs under PC stimulation. PCs were applied to all types of stem cells for 7 days, and then the levels of IL-10 (g) in culture supernatants were analyzed by enzyme-linked immunosorbent assays. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus MSC + PBS and MSC‐HO‐1 + PBS groups, #p < 0.05 versus MSC + PC group, and Δp < 0.05 versus MSC-HO-1 + PC group.
    PMID: 30362554
  • Sample type: Colorectal tissues
    Sample species: Rat
    Review: The anti-inflammatory cytokines IL-10 is decreased to a low level after TNBS exposure, whereas treatment with middle- or high-dose of BD-SNEDDS, BD-suspension, or AZA notably restored these anti-inflammatory cytokines. Data are expressed as mean±SD and analyzed by ANOVA followed by Dunnett’s test. ##P,0.01 vs intact group, **P,0.01 and *P,0.05 vs the vehicle group, P,0.05 and P,0.01 vs BD-suspension group.
    PMID: 30319255
  • Sample type: Tissues
    Sample species: Rat
    Review: IL-10 levels were obtained in treated and untreated groups (a) and comparison to the highest level obtained for each cytokine in periodontitis group (positive control).
    PMID: 30194012
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: Compared with the vector-NC group, decreased  IL-10 level were found in the P2X7-vector group (p < 0.05).
    PMID: 30892077
  • Sample type: Plamsa
    Sample species: Rat
    Review: Effect of KJL on IL-1 in plasma. Data are presented as the mean ± SD. ##P < 0.01, #P < 0.05 versus the control group; **P < 0.01, *P < 0.05 versus the TNBS group. (n = 8–12).
    PMID: 28750820
  • Sample type: Liver homogenate tissue
    Sample species: Rat
    Review: Effect of PTX on BDL-induced changes in liver IL-10, fourteen days post-operation, paclitaxel (PTX) (0.3 mg/kg/ip) was administered three times a week for 2 weeks. Hepatic IL-10 level were measured thereafter. Data are expressed as mean ± SD (n=6).
    PMID: 30243650
  • Sample type: Culture supernatants
    Sample species: Rat
    Review: Nrf2 knockdown reversed the anti-inflammatory effects of SalC. Nrf2 siRNA reversed SalC-induced IL‑10 downregulation. All data are presented as mean ± SD of four independent experiments. ##P < 0.01 vs control group, ⁎⁎P < 0.01 vs LPS group, scale bar = 20 μm.
    PMID: 30142530
  • Sample type: Jejunal mucosa tissues
    Sample species: Rat
    Review: Effect of ethanolamine on cytokines of weaned rats. The concentrations of cytokines in the jejunal mucosa were not different among groups.
    PMID: 29789027
  • Sample type: Brain tissues
    Sample species: Rat
    Review: Concentrations of the IL10 and their receptors were significantly lower in the serum than in the central nervous system in both SH and WKY rats (p < 0.001).
    PMID: 30539780

靶点详情

  • 最新研究进展：
    IL-10是一种免疫调节因子，能够抑制炎症反应和免疫细胞的激活。最近的研究表明，IL-10参与多种疾病的发生和发展，如炎症性肠病、自身免疫性疾病、感染性疾病等。此外，IL-10在某些疾病中也有治疗作用，如克罗恩病、银屑病等。
  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能参考文献：
    1. Low IL10 expression is associated with liver fibrosis. PMID: 30243650
    2. A modified pectic polysaccharide from turmeric (Curcuma longa) with antiulcer effects via anti-secretary, mucoprotective and IL-10 mediated anti-inflammatory mechanisms. PMID: 29924982
    3. IL10 suppressed inflammation, facilitated retinal pigment epithelium cell apoptosis and inhibited cell proliferation and migration through the regulation of VEGF expression in rhegmatogenous retinal detachment. PMID: 29568872
    4. ghrelin with high doses (80 and 160 mug/kg) significantly reduced pain intensity in both the early and the late phases of pain. The serum levels of cytokines, IL-10, and TGF-beta1 showed a significant elevation with ghrelin at the dose of 160 mug/kg. PMID: 27703278
    5. IL-10 not only attenuates neuronal apoptosis, but also promotes neurite outgrowth and synapse formation via the JAK1/STAT3 signaling pathway. PMID: 27456198
    6. both the AV and the LV vector systems were able to transfect limbal graft tissue ex vivo with biologically active IL-10, leading to delayed rejection compared to the controls. PMID: 28925732
    7. Addition of 320 (48) and 640 (96) mg/L (mg/kg BW) boron markedly reduced the serum IgG concentrations; splenic IL-2 and IL-10 expression; the number of CD3(+), CD4(+) and PCNA(+) cells; and increased the number of splenic CD8(+) and caspase-3(+) cells and promoted caspase-3 expression in CD3(+) cells. PMID: 28092075
    8. The expression levels of IL-10, NF-kappaB and MMP-3 in the intervertebral disc tissue samples were higher in test animals than in controls at one-month, three-months and six-months after surgically induced cervical spondylosis. Cervical degeneration may be accompanied by changes in IL-10. PMID: 29197741
    9. Comparative Modulation of Levels of Oxidative Stress in the Liver of Anti-Tuberculosis Drug Treated Wistar Rats by Vitamin B12, Beta-Carotene, and Spirulina fusiformis: Role of NF-kappaB, iNOS, IL-6, and IL-10.( PMID: 28387444
    10. the effects of dapagliflozin on STAT3 activity and myocardial IL-10 levels can be reversed by 3-morpholinosydnonimine, a peroxynitrite generator. Taken together, these observations provide a novel mechanism of SGLT2 inhibitors-mediated M2 polarization through a RONS-dependent STAT3-mediated pathway and selective SGLT2 inhibitors are more effective in attenuating myofibroblast infiltration during postinfarction remodeling PMID: 28132924
    11. TNF-alpha, IL-6 and IL-10 expressions, responsible for disparity in action of curcumin against cisplatin-induced nephrotoxicity in rats. PMID: 28258441
    12. this study shows that transduction of interleukin-10 through renal artery attenuates vascular neointimal proliferation and infiltration of immune cells in rat renal allograft PMID: 27317647
    13. this study shows that that N-Acetyl-l-cysteine acts as an antipyretic via IL-10 stimulation PMID: 27363620
    14. Transplantation of mesenchymal stem cells overexpressing IL10 preserves myocardial viability and left ventricular rfunction in rats with myocardial infarction. PMID: 28322445
    15. The bactericidal activity of caspase-6-/- macrophages was impaired compared to wild type cells. Caspase-6-/- mice showed higher expression of the IL-1b gene, known to be detrimental in murine melioidosis. Expression of the IL-10 gene was also increased in caspase-6-/- mice as early as 6 hours after infection. Treatment with exogenous IL-10 rendered mice more susceptible against B. pseudomallei challenge PMID: 28686630
    16. use of rebamipide retards CCl4-induced hepatic fibrosis, which is associated with significant increases in liver levels of IL-10 PMID: 26849241
    17. Data suggest that vitamin A, when used as an unnecessary dietary supplement, decreases skeletal muscle expression of anti-inflammatory mediators interleukin-10 and heat shock protein 70, important factors for positive exercise adaptations and tissue damage prevention. PMID: 28368329
    18. Exercise combined with ultrasound attenuates neuropathic pain in rats and is associated with downregulation of IL-6 and TNF-alpha, and with upregulation of IL-10. PMID: 28319508
    19. Results uncover a novel neuroimmune modulation of GABAergic neurotransmission as well as a novel role for IL-10 in response to acute ethanol intake. Early increase in IL-10 levels may contribute to the complex changes in neuroimmune signaling that occur after acute exposure to ethanol. Further, IL-10 has an inhibitory effect on GABAergic transmission, possibly leading to inhibition of sleep PMID: 27016017
    20. Nonsteroidal Anti-Inflammatory Drugs data provide opportunities to investigate such associations and specifically imply that potential interrelationships among OTC NSAID use, IL-10 genotype, and IL-10 cytokine levels deserve further study PMID: 28135825
    21. The expression of IL-10 and TGF-beta in the CD25 siRNA group increased, while IFN-gamma, IL-1beta, and TNF-alpha expression decreased, as well as no significant changes in Foxp3 expression were observed at day 14 post-operation. PMID: 27567557
    22. IL10 has a role in preventing -induced cell death in rat hippocampal cultures PMID: 28063948
    23. IL-10 592 polymorphism was associated with diabetic retinopathy susceptibility, suggesting that the gene polymorphism might be a risk factor for diabetic retinopathy PMID: 26492380
    24. Mesenchymal stromal cells inhibit Human antigen R (HuR) trascription, an enhancer of RENmRNA stability by IL10 release PMID: 26866372
    25. Mesenchymal stem cells decrease apoptosis and improve learning-memory function in hypoxic-ischemic brain damage rats by suppressing the TLR2/NFkappaB signaling pathway via a feedback mechanism that reduces IL-10 release. PMID: 26475712
    26. Green tea intake reduces expression of the pro-inflammatory cytokine TNF-alpha and the osteoclastogenic mediator RANKL to normal levels while increasing expression of the anti-inflammatory cytokine IL-10. PMID: 26270535
    27. IL-10 released from activated glial cells in the VLO can potentially attenuate allodynia while IL-1beta released from activated glial cells in the VLO can potentially maintain or facilitate allodynia PMID: 25617163
    28. The objectives of these studies were to investigate the responses of IL-10 receptors (IL-10Rs) and IL-10 signalling proteins (IL-10SPs) in hyperoxic foetal alveolar type II cells. PMID: 26059905
    29. Leydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. PMID: 25598450
    30. Exercise increased the amount of IL-1beta and IL-10 positive chondrocytes PMID: 25702530
    31. Study shows that, in addition to inducing persistent memory deficits, maternal deprivation increased the levels of the anti-inflammatory cytokine, IL-10, and of the pro-inflammatory cytokine, TNF-alpha, and decreased the levels of BDNF in the adulthood PMID: 25182413
    32. atropine in malathion intoxicated rats had no effect on suppression of the synthesis of proinflammatory cytokines TNF, IL-1g and IL-6 as well as the content of anti-inflammatory cytokines IL-10 and IL-13. PMID: 26591203
    33. Short-term but not long-term hypoglycaemia enhances plasma levels and hepatic expression of HSP72 in insulin-treated rats: an effect associated with increased IL-6 levels but not with IL-10 or TNF-alpha. PMID: 25096025
    34. the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats PMID: 25009084
    35. The dysfunction of dendritic cells and the increase of IL-10-producing Breg and Foxp3(+) Treg might play important roles in hepatocellular carcinoma progression PMID: 25066760
    36. SOCS-3 was involved in cerebral vasospasm after subarachnoid hemorrhage via an IL-6- and IL-10-related mechanism. IL-10 levels increased during the first 6 h after SAH, after which this increase tapered off. PMID: 24463741
    37. results suggest that IL-10/HMOX1 signaling is involved in modulation of cochlear inflammation through inhibition of MCP-1/CCL2 regulation in SLFs PMID: 25780042
    38. The neuronal apoptosis rate was positively correlated with the levels of TNF-alpha and IL-6, and negatively correlated with IL-10 and GDNF. PMID: 24706318
    39. cells from long-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1beta mRNA relative to adult rats. PMID: 25081109
    40. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10. PMID: 25329858
    41. Regulation of IL-10 by chondroitinase ABC promotes a distinct immune response following spinal cord injury PMID: 25471580
    42. We found cardiac and histological changes compatible with the inflammatory process triggered by acute pancreatitis with the promotion of local myocardial cytokine production. PMID: 24865534
    43. study provides novel evidence that IL-10 plays an up-regulatory role in the anti-hypertensive activity of CCL5 in SHR VSMCs PMID: 24656930
    44. The high expression in IL-1beta and IL-10 levels in small bowel and colon might explain the accelerated healing process in these wounds in comparison to skin and muscle tissues. PMID: 24114903
    45. Daily treatment with anti-IL10 antibody significantly increased paw edema. PMID: 23765634
    46. Prevention of acute liver allograft rejection by IL-10-engineered mesenchymal stem cells. PMID: 24527865
    47. Gemfibrozil plus metformin decrease MMP-9, increase IL-10 and adiponectin acting. PMID: 23111552
    48. Pro-inflammatory cytokines from area postrema and organum vasculosum laminae terminalis are modulated by IL-10 antibodies. PMID: 23388469
    49. GSK-3beta modulates inflammatory IL-10 levels released from cortical glia. PMID: 22796213
    50. These data in cardiomyocytes suggest that IL-10 induced anti-apoptotic signaling involves upregulation of TLR4 through MyD88 activation. PMID: 23141143

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 数据库链接：

    KEGG: rno:25325

    STRING: 10116.ENSRNOP00000006246

    UniGene: Rn.9868