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Rat Interleukin 1β,IL-1β ELISA Kit

  • 中文名称：
    大鼠白介素1β (IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E08055r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL1B (白细胞介素-1β，Interleukin-1β) 是由许多不同类型的细胞产生的促炎症细胞因子，包括单核细胞、巨噬细胞、树突状细胞、T细胞和B细胞等。IL1B 参与了许多生理和病理过程，包括炎症、感染、肿瘤、自身免疫疾病等。
        j9九游会登录入口首页生物所提供的Rat Interleukin 1β,IL-1β ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的IL1B，其灵敏度为0.039 ng/ml，检测范围为0.156 ng/ml-10 ng/ml。
     
  • 别名：
    Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.156 ng/ml-10 ng/ml.
  • 灵敏度：
    0.039 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    88

    Range %

    80-96

    1:2

    Average %

    92

    Range %

    90-105

    1:4

    Average %

    94

    Range %

    91-103

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    99

    90-105

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.903

    2.834

    2.868

    2.689

    5

    2.208

    2.167

    2.187

    2.008

    2.5

    1.563

    1.508

    1.536

    1.356

    1.25

    0.872

    0.859

    0.865

    0.686

    0.625

    0.579

    0.551

    0.565

    0.386

    0.312

    0.363

    0.353

    0.358

    0.178

    0.156

    0.284

    0.274

    0.279

    0.099

    0

    0.184

    0.175

    0.180

    　

  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：组织匀浆液 心

样品信息：大鼠

稀释比：没有稀释

产品评价： 该试剂盒操作简便，检测快速准确。试剂性能稳定，能满足不同场景的检测需求。包装严实，标识清晰。价格合理，性价比高。无论是专业机构还是个人使用，都能提供可靠的检测结果，为健康保驾护航。

By 徐老师

靶点详情

  • 最新研究进展：
    IL-1β是一种由单核细胞和巨噬细胞产生的细胞因子，能够介导炎症和免疫反应。最新研究发现，IL-1β参与多种疾病的发生和发展，包括肿瘤、自身免疫病和神经退行性疾病等。目前已有研究在探索针对IL-1β的治疗方案，如使用IL-1β抑制剂等。此外，一些研究还发现IL-1β的多态性可能与特定疾病的风险相关。
  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：