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Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名称：
    人β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
  • 货号：
    CSB-E10684h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        Aβ1-42是beta淀粉样蛋白（Aβ）的一种形式，它是一种由神经元合成和分泌的蛋白质。Aβ1-42是导致阿尔茨海默病（AD）的重要成分之一。它在AD病变过程中聚集成胶质斑和神经纤维缠结，损害神经元的正常功能。此外，Aβ1-42还可能在其他神经系统疾病的发病机制中发挥作用，包括创伤性脑损伤、脊髓灰质炎和肌萎缩侧索硬化症等。
        j9九游会登录入口首页生物所提供的Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆、脑脊液(CSF)样本中的Aβ1-42，其灵敏度为0.078 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 缩写：
    Aβ1-42
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-92  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 97  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.302 2.379 2.341 2.244  
    10 1.762 1.781 1.772 1.675  
    5 1.124 1.189 1.157 1.060  
    2.5 0.653 0.679 0.666 0.569  
    1.25 0.375 0.398 0.387 0.290  
    0.625 0.251 0.259 0.255 0.158  
    0.312 0.151 0.153 0.152 0.055  
    0 0.095 0.098 0.097 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human Aβ1-42 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled Aβ1-42 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

We are interested in following item.
#CSB-E10684h
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

A:
Thanks for your inquiry.Regarding the mentioned kit,we would like to inform the following information:
(Note:Capture:capture antibody coated on the plate;Detection: biotin-conjugated antibody)
#CSB-E10684h,Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody

靶点详情

  • 最新研究进展：
    Aβ1-42是β淀粉样蛋白（amyloid beta protein，Aβ）的一种亚型，它是导致阿尔茨海默病（Alzheimer's disease，AD）的主要病理因素之一。最新的研究表明，Aβ1-42的聚集和沉积不仅在AD中发挥作用，而且在其他神经退行性疾病中也可能具有重要作用，如前额颞叶痴呆症、帕金森氏病等。