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  4. Human Ras-related protein Rab-10(RAB10) ELISA kit

Human Ras-related protein Rab-10(RAB10) ELISA kit

  • 中文名称：
    人Ras相关蛋白Rab-10(RAB10)酶联免疫试剂盒
  • 货号：
    CSB-EL019152HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human RAB10 ELISA Kit was designed for the quantitative measurement of Human RAB10 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.77 pg/mL.

  • 别名：
    GTP binding protein RAB10 ELISA Kit; Rab10 ELISA Kit; RAB10 member RAS oncogene family ELISA Kit; RAB10_HUMAN ELISA Kit; Ras related GTP binding protein ELISA Kit; Ras related GTP binding protein RAB10 ELISA Kit; Ras-related protein Rab-10 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.77 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human RAB10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 89-99
    1:2 Average % 95
    Range % 90-99
    1:4 Average % 92
    Range % 87-98
    1:8 Average % 97
    Range % 92-103
  • 回收率：
    The recovery of human RAB10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 98-105
    EDTA plasma (n=4) 92 86-97
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2500 2.186 2.230 2.208 2.041
    1250 1.724 1.619 1.672 1.505
    625 1.223 1.165 1.194 1.027
    312.5 0.744 0.753 0.749 0.582
    156.25 0.516 0.503 0.510 0.343
    78 0.368 0.378 0.373 0.206
    39 0.259 0.250 0.255 0.088
    0 0.166 0.168 0.167 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane. In epithelial cells, it regulates transport from the Golgi to the basolateral membrane. May play a role in the basolateral recycling pathway and in phagosome maturation. May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis.; (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.
  • 基因功能参考文献：
    1. miR378a3p may act as a tumorsuppressor in Esophageal squamous cell carcinoma cells through negatively regulating Rab10. PMID: 29693138
    2. Interrogating Parkinson's disease LRRK2 kinase pathway activity by assessing Rab10 phosphorylation in human neutrophils has been reported. PMID: 29127255
    3. Our results suggest that RAB10 could be a promising therapeutic target for Alzheimer's Disease prevention PMID: 29183403
    4. RAB10 regulates cell survival and proliferation through multiple oncogenic, cell stress and apoptosis pathways. More importantly, high RAB10 expression levels in HCC cells correlated with a poor prognosis in HCC patients. PMID: 28460436
    5. miR-329 also suppresses wound-healing and migration ability of osteosarcoma cells and inhibits tumorigenicity in vivo. Rab10 was identified as a target of miR-329 in osteosarcoma and mediates its biofunction PMID: 27487475
    6. Data suggest that MGCRABGAP is involved in mammalian spermiogenesis by modulating RAB10. PMID: 28067790
    7. the target gene RAB10 regulated by miRNA-369-3p, miRNA-30e-5p, miRNA-30e-3p, and miRNA-655 may play key role in the progression and development of AD. PMID: 26082458
    8. The Anaplasma phagocytophilum surface protein, uridine monophosphate kinase, was identified as a guanine nucleotide-independent, Rab10-specific ligand. PMID: 26289115
    9. Rab10 is a target of the GTPase-activating protein AS160, which is inhibited after phosphorylation by the protein kinase Akt. PMID: 25103239
    10. Rab10 silencing increased the plasma membrane residence of HAS3, resulting in a significant increase of hyaluronan secretion and an enlarged cell surface HA coat, whereas Rab10 overexpression suppressed hyaluronan synthesis. PMID: 24509846
    11. Rab10 regulates endoplasmic reticulum dynamics/morphology. Suggest that these dynamics could be coupled to phospholipid synthesis. PMID: 23263280
    12. Insulin-stimulated GLUT4 protein translocation in adipocytes requires the Rab10 guanine nucleotide exchange factor Dennd4C PMID: 21454697
    13. Rab10 and Rab8A are new cytoplasmic factors implicated in WPB biogenesis that play a role in generating granules that can rapidly respond to secretagogue PMID: 21070595
    14. Rab10, acting upstream of Rab5, plays a prominent role in phagolysosome formation and can modulate Mycobacterium-containing phagosomes maturation PMID: 20028485
    15. Fibronectin 1 and RAB10 show elevated expression in uninvolved psoriatic epidermis. PMID: 15855153
    16. a related Rab protein, Rab10, can interact with myosin Va, myosin Vb, and myosin Vc PMID: 19008234

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  • 亚细胞定位：
    Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cytoplasm, cytoskeleton, cilium basal body. Endoplasmic reticulum membrane. Cytoplasm, perinuclear region.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Expressed in the hippocampus. Expressed in neutrophils (at protein level).
  • 数据库链接：

    HGNC: 9759

    OMIM: 612672

    KEGG: hsa:10890

    STRING: 9606.ENSP00000264710

    UniGene: Hs.467960