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  4. Human Interleukin 23,IL-23 ELISA Kit

Human Interleukin 23,IL-23 ELISA Kit

  • 中文名称：
    人白介素23(IL-23)酶联免疫试剂盒
  • 货号：
    CSB-E08461h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    The human IL-23 ELISA Kit is engineered for accurate measurement of human IL-23 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-23 content in the sample. The color intensity is positively correlated with IL-23 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    IL-23 is a heterodimeric cytokine consisting of an IL-12p40 subunit and an IL-23-specific p19 subunit. It is secreted by activated dendritic cells and macrophages and can specifically amplify and stabilize the proliferation of IL-17-secreting Th17 cells. The IL-23/Th17 pathway has been involved in the development of many autoimmune diseases, such as rheumatoid arthritis (RA), systemic lupus erythematosus, and psoriatic arthritis (PsA). IL-23 signals through the IL-23R in complex with IL-12Rβ1, inducing the generation of secreted mediators such as IL-17 and IL-22, in pro-inflammatory, IL-17-secreting TCRγδ (Tγδ17), and in Th17 cells. These pro-inflammatory mediators facilitate the rapid recruitment and activation of granulocytes and macrophages that eventually result in the development of clinical symptoms.

  • 别名：
    N/A
  • 缩写：
    IL-23
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %98
    Range %92-106
    1:4Average %94
    Range %90-98
    1:8Average %95
    Range %89-99
  • 回收率：
    The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8982-96
    EDTA plasma (n=4)9389-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.608 2.507 2.558 2.415
    2001.865 1.764 1.815 1.672
    1001.207 1.186 1.197 1.054
    500.801 0.790 0.796 0.653
    250.437 0.448 0.443 0.300
    12.50.301 0.312 0.307 0.164
    6.250.228 0.234 0.231 0.088
    00.142 0.143 0.143 　
  • 数据处理：
  • 货期：
    3-5 working days

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产品评价

问答及客户评论

■ 常见问题解答
Q:

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

A:
Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit
If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!
Q:

I am interested in this kit. I would like to know which subunit of IL-23 can be detected by the kit.
Please provide a detailed information about kit’s components:
a. Capture antibody or capture antigen.
b. Detection antibody (if applicable).
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.

A:
Thanks for your inquiry.
Interleukin-23 (IL-23) is a heterodimeric cytokine composed of an IL-12p40 subunit (that is shared with IL-12 ) and the IL-23p19 subunit.
CSB-E08461h
This kit detects IL23（it is compound）.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is a dimer of recombinant proteins expressed by eukaryotic cells in vitro.
Q:

Thank you for your assistance.
Is the 293 protein you are referring the IL-23 p19 subunit?
Because you just mention the immunogen is 293 cell-derived recombinant protein. Please clarify, what is the 293 cell-derived protein specifically? For example, is it the IL-23 p19 subunit?
It is good to know, if the antibodies bind to the p40 unit or the p19 subunit?
Looking forward to receiving your feedback.

A:
Thanks for your further inquiry. There are 2 antibodies in the kit, so there are 2 immunogens. They are designed against the 2 subunits of the compound.
Sorry we can not disclose which antibody is specific to which protein.