Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

  • 中文名称：
    鱼细胞色素P450 1A1(CYP1A1)酶联免疫试剂盒
  • 货号：
    CSB-EL006395FI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish CYP1A1 ELISA Kit was designed for the quantitative measurement of Fish CYP1A1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 75 pg/mL-1200 pg/mL and the sensitivity is 75 pg/mL.

  • 别名：
    cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
  • 缩写：
    CYP1A1
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    75 pg/mL-1200 pg/mL
  • 灵敏度：
    75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-98  
    1:2 Average % 100  
    Range % 92-107  
    1:4 Average % 85  
    Range % 80-91  
    1:8 Average % 88  
    Range % 83-96  
  • 回收率：
    The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 88-95  
    EDTA plasma (n=4) 94 91-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1200 0.146 0.147 0.147    
    600 0.238 0.254 0.246    
    300 0.524 0.553 0.539    
    150 1.112 1.135 1.124    
    75 2.338 2.277 2.308    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：