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Rat Aspartate Aminotransferase (AST) ELISA kit

  • 中文名称：
    大鼠天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒
  • 货号：
    CSB-E13023r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This rat AST ELISA Kit is suitable for qualitatively determining AST concentrations in multiple biological fluids, including rat serum, plasma, and tissue homogenates in vitro. AST, also called GOT1, is an important transaminase enzyme in amino acid metabolism that catalyzes the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate. AST is a part of the malate-aspartate shuttle in the myocardium and participates in gluconeogenesis in the liver and kidney, glyceroneogenesis in the adipose tissue, and the production of neurotransmitters and neuro-glial pathways in the brain. A high AST level in the body can be an indicator of liver disease and other health problems.

    This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of rat AST in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-rat AST antibody. Biotin-labeled AST antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the AST in the rat samples. AST binds to the biotinylated anti-rat AST monoclonal antibody. And the biotin on the biotinylated anti-rat AST monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of AST in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

  • 别名：
    Got1Aspartate aminotransferase ELISA kit; cytoplasmic ELISA kit; cAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.3 ELISA kit; Cysteine aminotransferase ELISA kit; cytoplasmic ELISA kit; Cysteine transaminase ELISA kit; cytoplasmic ELISA kit; cCAT ELISA kit; Glutamate oxaloacetate transaminase 1 ELISA kit; Transaminase A ELISA kit
  • 缩写：
    GOT1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.12 mIU/mL-200 mIU/mL
  • 灵敏度：
    0.78 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-92
    1:2Average %104
    Range %100-107
    1:4Average %93
    Range %88-97
    1:8Average %98
    Range %92-103
  • 回收率：
    The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9591-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    2002.094 2.068 2.081 1.918
    1001.276 1.310 1.293 1.130
    500.838 0.784 0.811 0.648
    250.521 0.539 0.530 0.367
    12.50.353 0.334 0.344 0.181
    6.250.300 0.288 0.294 0.131
    3.120.241 0.237 0.239 0.076
    00.164 0.162 0.163 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since we cannot collect more than 150 µL of blood from mice, we can use only 50 µL serum for ELISA. Will it fall within the detectable range ?

A:
We are glad to inform you the following details:
CSB-E13023r,Rat Aspartate Aminotransferase (AST) ELISA kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
If any further questions,pls let me know.

靶点详情

  • 功能：
    Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
  • 基因功能参考文献：
    1. Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, mercaptopyruvate sulfurtransferase (MPST)and cysteine aminotransferase (CAT). PMID: 24611772
    2. In vivo lentiviral gene delivery of GOT minimized lesion volume, whereas GOT knockdown worsened stroke outcomes. Thus, brain tissue GOT emerges as a novel target in managing stroke outcomes. PMID: 21361730
    3. Results suggest that downregulation of the endogenous SO(2)/GOT pathway is likely involved in the pathogenesis of ISO-induced myocardial injury. PMID: 20733562
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 组织特异性：
    Expressed in liver and kidney.
  • 数据库链接：

    KEGG: rno:24401

    STRING: 10116.ENSRNOP00000022309

    UniGene: Rn.5819