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  4. Rat heart fatty acid binding protein(h-FABP)ELISA Kit

Rat heart fatty acid binding protein(h-FABP)ELISA Kit

  • 中文名称：
    大鼠心型脂肪酸结合蛋白(h-FABP)酶联免疫试剂盒
  • 货号：
    CSB-E16184r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rat heart fatty acid-binding protein (h-FABP) ELISA Kit is used to quantitatively measure rat h-FABP levels in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of h-FABP in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

    h-FABP, also called FABP3, is ubiquitously expressed in the heart, skeletal muscle, and other tissues. It participates in maintaining an energy supply to the heart and other body parts, as well as in regulating the intramuscular fat content and improving insulin sensitivity. FABP3 binds to free long-chain fatty acids (LCFAs) and transports them for cell metabolism, thus protecting against lipid toxicity. Studies have demonstrated that deficiencies in FABP3 exacerbate metabolic derangement in cardiac Hypertrophy and heart failure via the PPARα pathway.

  • 别名：
    Fabp3Fatty acid-binding protein ELISA kit; heart ELISA kit; Fatty acid-binding protein 3 ELISA kit; Heart-type fatty acid-binding protein ELISA kit; H-FABP ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %84-92
    1:2Average %96
    Range %91-102
    1:4Average %84
    Range %81-88
    1:8Average %94
    Range %90-98
  • 回收率：
    The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-95
    EDTA plasma (n=4)10395-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.095 2.086 2.091 1.967
    5001.550 1.648 1.599 1.475
    2501.121 1.156 1.139 1.015
    1250.728 0.751 0.740 0.616
    62.50.417 0.403 0.410 0.286
    31.20.241 0.237 0.239 0.115
    15.60.211 0.214 0.213 0.089
    00.119 0.129 0.124 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
  • 基因功能参考文献：
    1. Significant down-regulation of heart-type fatty acid binding protein after myocardial ischemia might play an important role in myocardial injury and energy metabolism disorder. PMID: 21155221
    2. H-FABP might have potential to be an early and quantitative parameter of clinical severity and prognosis in CO poisoning. PMID: 20375122
    3. A complex interrelationship between glucose and fatty acids in the control of FABP gene expression; FABP3 and 5 may play a role in insulin secretion. PMID: 21099311
    4. rat myocardial FABP gene maps to chromosome 5q36 and was detected in a preparation of total RNA isolated from adult rat heart PMID: 10561574
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Calycin superfamily, Fatty-acid binding protein (FABP) family
  • 组织特异性：
    Heart, but also skeletal muscle, kidney, brain and mammary gland.
  • 数据库链接：