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  4. Human Polycomb complex protein BMI-1(BMI1) ELISA kit

Human Polycomb complex protein BMI-1(BMI1) ELISA kit

  • 中文名称：
    人多梳复合蛋白BMI-1(BMI1)酶联免疫试剂盒
  • 货号：
    CSB-EL002726HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human BMI1 ELISA Kit was designed for the quantitative measurement of Human BMI1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    B lymphoma Mo MLV insertion region (mouse) ELISA Kit; B lymphoma Mo MLV insertion region 1 homolog ELISA Kit; Bmi 1 ELISA Kit; BMI1 ELISA Kit; BMI1 polycomb ring finger oncogene ELISA Kit; BMI1_HUMAN ELISA Kit; Flvi 2/bmi 1 ELISA Kit; FLVI2/BMI1 ELISA Kit; MGC12685 ELISA Kit; Murine leukemia viral (bmi 1) oncogene homolog ELISA Kit; Oncogene BMI 1 ELISA Kit; PCGF 4 ELISA Kit; PCGF4 ELISA Kit; Polycomb complex protein BMI 1 ELISA Kit; Polycomb complex protein BMI-1 ELISA Kit; Polycomb group protein Bmi1 ELISA Kit; Polycomb group ring finger 4 ELISA Kit; Polycomb group RING finger protein 4 ELISA Kit; RING finger protein 51 ELISA Kit; RNF 51 ELISA Kit; RNF51 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    25 pg/mL-1600 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BMI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-93
    1:2 Average % 95
    Range % 90-100
    1:4 Average % 103
    Range % 99-107
    1:8 Average % 90
    Range % 85-95
  • 回收率：
    The recovery of human BMI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 85 80-89
    EDTA plasma (n=4) 94 90-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.768 1.896 1.832 1.714
    800 1.169 1.203 1.186 1.068
    400 0.739 0.704 0.722 0.604
    200 0.472 0.457 0.465 0.347
    100 0.320 0.340 0.330 0.212
    50 0.267 0.271 0.269 0.151
    25 0.195 0.182 0.189 0.071
    0 0.121 0.115 0.118 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 数据库链接：

    HGNC: 1066

    OMIM: 164831

    KEGG: hsa:100532731

    STRING: 9606.ENSP00000365851

    UniGene: Hs.380403