Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Dog S100 calcium binding protein B (S100B) ELISA kit

Dog S100 calcium binding protein B (S100B) ELISA kit

  • 中文名称：
    犬S100B蛋白(S100B)酶联免疫试剂盒
  • 货号：
    CSB-EL020643DO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 缩写：
    S100B
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 pg/mL-2500 pg/mL
  • 灵敏度：
    9.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 85    
    Range % 80-91    
    1:2 Average % 93    
    Range % 87-98    
    1:4 Average % 102    
    Range % 98-106    
    1:8 Average % 98    
    Range % 94-103    
  • 回收率：
    The recovery of pig S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 89-102    
    EDTA plasma (n=4) 89 84-95    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    2500 1.955 2.021 1.988 1.816    
    1250 1.474 1.522 1.498 1.326    
    625 0.911 0.987 0.949 0.777    
    312.5 0.526 0.536 0.531 0.359    
    156.2 0.365 0.345 0.355 0.183    
    78.1 0.293 0.312 0.303 0.131    
    39 0.221 0.226 0.224 0.052    
    0 0.175 0.169 0.172 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价