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  4. Recombinant Rat Transient receptor potential cation channel subfamily V member 2 (Trpv2)

Recombinant Rat Transient receptor potential cation channel subfamily V member 2 (Trpv2)

In Stock
  • 货号：
    CSB-CF893416RA
  • 规格：
    ￥12960
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    88.2 kDa
  • 表达区域：
    1-761aa
  • 氨基酸序列
    MTSASSPPAFRLETSDGDEEGNAEVNKGKQEPPPMESPFQREDRNSSPQIKVNLNFIKRPPKNTSAPSQQEPDRFDRDRLFSVVSRGVPEELTGLLEYLRWNSKYLTDSAYTEGSTGKTCLMKAVLNLQDGVNACIMPLLQIDKDSGNPKLLVNAQCTDEFYQGHSALHIAIEKRSLQCVKLLVENGADVHLRACGRFFQKHQGTCFYFGELPLSLAACTKQWDVVTYLLENPHQPASLEATDSLGNTVLHALVMIADNSPENSALVIHMYDGLLQMGARLCPTVQLEEISNHQGLTPLKLAAKEGKIEIFRHILQREFSGPYQPLSRKFTEWCYGPVRVSLYDLSSVDSWEKNSVLEIIAFHCKSPNRHRMVVLEPLNKLLQEKWDRLVSRFFFNFACYLVYMFIFTVVAYHQPSLDQPAIPSSKATFGESMLLLGHILILLGGIYLLLGQLWYFWRRRLFIWISFMDSYFEILFLLQALLTVLSQVLRFMETEWYLPLLVLSLVLGWLNLLYYTRGFQHTGIYSVMIQKVILRDLLRFLLVYLVFLFGFAVALVSLSREARSPKAPEDNNSTVTEQPTVGQEEEPAPYRSILDASLELFKFTIGMGELAFQEQLRFRGVVLLLLLAYVLLTYVLLLNMLIALMSETVNHVADNSWSIWKLQKAISVLEMENGYWWCRRKKHREGRLLKVGTRGDGTPDERWCFRVEEVNWAAWEKTLPTLSEDPSGPGITGNKKNPTSKPGKNSASEEDHLPLQVLQSP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH.
  • 基因功能参考文献：
    1. TRPV2 sensitization plays a key role in intra-oral heat and mechanical hypersensitivity after the incision. PMID: 29505690
    2. blocking the TRPV2 induced NGF secretion via the mitogen-activated protein kinase (MAPK)-JNK signaling pathway PMID: 27468746
    3. Mechanical stimuli dramatically increased growth cone motility and caused actin reorganization via activation of TRPV2. TRPV2 physically interacts with actin. PMID: 28007781
    4. retinal VSMCs express a range of mechanosensitive TRP channels, but only TRPV2 appears to contribute to myogenic signaling in this vascular bed. PMID: 27784066
    5. heat sensitivity of the TRPV2 channel PMID: 27074678
    6. the structure of full-length TRPV2 at approximately 5 A resolution by cryo-electron microscopy, is reported. PMID: 27021073
    7. This study evaluated the role of Trpv2 in arthritis and the pharmacological effects of Trpv2 agonists. PMID: 25869297
    8. Demonstrated the co-translational insertion of the membrane-embedded region of the TRPV2 and its disposition in biological membranes, identifying that TM1-TM4 and TM5-TM6 regions can assemble as independent folding domains. PMID: 25956061
    9. TRPV2 is highly expressed on the peri-infarct infiltrating macrophages and may play an important role in post-myocardial infarction phagocytosis. PMID: 25136832
    10. cellular function of TRPV2 channel PMID: 24392006
    11. The present study suggests that TRPV1- and TRPV2-immunoreactive jugular-petrosal neurons may be associated with the regulation of the swallowing reflex. PMID: 23584686
    12. TRPV2 participates in strain-induced Ca(2 ) entry in alveolar type II cells. PMID: 22190268
    13. TRPV2 may play an important role in several central nervous system networks that regulate body fluid homeostasis, autonomic function, and metabolism. PMID: 22750329
    14. data suggest that regionally specific changes in TRPV expression in the magnocellular neurosecretory cell AVP neurones could alter their osmosensing ability. PMID: 22188460
    15. Transient receptor potential vanilloid-related channel (TRPV)2 is suggested as the thromboxane A2-activated nonselective cation channel involved in rat pulmonary arterial smooth muscle cells under hypoxia. PMID: 21998141
    16. Polymerase chain reaction used to check the expression of all TRPM and TRPV channel members with specific primers. PMID: 17712480
    17. key amino acids, Arg-114 and Glu-761, in the N- and C-cytosolic tails, respectively, that determine ligand binding were determined in VRL1 PMID: 12228246
    18. the heart is innervated by VR1-expressing afferent nerves and these afferent nerves are essential for the cardiogenic sympathoexcitatory reflex during myocardial ischaemia PMID: 12829722
    19. Co-expression of VR1 and VRL-1 neurons is more abundant in the nodose ganglion than in the jugular ganglion and suggests that co-expression of VR1 and VRL-1 may be more common in visceral sensory neurons than in somatic sensory neurons. PMID: 12867271
    20. analysis of glycosylation and localization in comparison to the endogenously expressed protein in F-11 cells PMID: 14622291
    21. Slowly activating heat-evoked currents correspond to the type I heat responses of primary nociceptive afferents, which possibly are mediated by TRPV2. PMID: 14625457
    22. The present study indicates that P2X3 receptor-ir TG neurons, which co-express VR-ir, are abundant in the facial skin. The tooth pulp is probably innervated by TG neurons, which contain both P2X3-and VRL-1-ir. PMID: 14725976
    23. VRL-1, in contrast to VR1, is present in a diverse population of spinal cord neurons and undoubtedly contributes to numerous functions in addition to nociceptive processing. PMID: 14961565
    24. novel protein-protein interaction with recombination activating gene 1 PMID: 14991772
    25. VRL1-immunoreactive cell bodies are present throughout the rat retina, represented by amacrine cells in the inner nuclear layer and ganglion cell layer. PMID: 15174083
    26. TRPV2 and PKA have roles as a signaling module for transduction of physical stimuli in mast cells PMID: 15249591
    27. Normal heat responses are present in IB4-positive neurons in the absence of TRPV2. PMID: 15254097
    28. recombination activating gene 1 participates in the control of TRPV2 surface levels PMID: 15547947
    29. VRL1 immunoreactivity were observed in the intraepithelial free nerve endings, subepithelial nerve plexus and laryngeal epithelial cells. PMID: 15620571
    30. TRPV1, V2, and V3 channels are expressed on these sensory neurons and their terminals. PMID: 15653710
    31. It is concluded that PI3-kinase induces or modulates the activity of recombinant TRPV2 channels; in contrast to the previously proposed mechanism, activation of TRPV2 channels by PI3-kinase is not due to channel translocation to the plasma membrane. PMID: 16533525
    32. only nociceptors that expressed TRPV2, alone or in combination with TRPV1, exhibited heat sensitization in skin PMID: 17287441
    33. Our findings support the hypothesis that increased TRP channel expression in sensory neurons may contribute to mechanical and cold hypersensitivity. PMID: 17517374
    34. TRPV2, a temperature-gated channel, is highly expressed in junctional epithelial cells, but not in oral sulcular epithelial cells or oral epithelial cells. PMID: 19579031
    35. Results show that the patterns of distribution of TRPV1 and TRPV2 are different during the development of the rat retina, suggesting that they have specific roles in both visual processing and in providing specific cues to neural development. PMID: 19619635

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cytoplasm. Melanosome.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, TrpV subfamily, TRPV2 sub-subfamily
  • 组织特异性：
    Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level).
  • 数据库链接：

    KEGG: rno:29465

    STRING: 10116.ENSRNOP00000004248

    UniGene: Rn.206528