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  4. Recombinant Rat Orexin receptor type 2 (Hcrtr2)

Recombinant Rat Orexin receptor type 2 (Hcrtr2)

  • 中文名称：
    大鼠Hcrtr2重组蛋白
  • 货号：
    CSB-CF010232RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Hcrtr2; Orexin receptor type 2; Ox-2-R; Ox2-R; Ox2R; Hypocretin receptor type 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-460
  • 氨基酸序列
    MSSTKLEDSLPRRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAG YIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETW FFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVVIWIVS CIIMIPQAIVMERSSMLPGLANKTTLFTVCDERWGGEVYPKMYHICFFLVTYMAPLCLMV LAYLQIFRKLWCRQIPGTSSVVQRKWKQPQPVSQPRGSGQQSKARISAVAAEIKQIRARR KTARMLMVVLLVFAICYLPISILNVLKRVFGMFTHTEDRETVYAWFTFSHWLVYANSAAN PIIYNFLSGKFREEFKAAFSCCLGVHRRQGDRLARGRTSTESRKSLTTQISNFDNVSKLS EHVALTSISTLPAANGAGPLQNWYLQQGVPSSLLSTWLEV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.
  • 基因功能参考文献：
    1. Study using specific orexin receptor antagonists suggests that the orexin 2 receptor has a dominant role in the observed effects of orexin A/hypocretin-1 . To our knowledge, this article is the first to show orexinergic modulation of the ventrolateral geniculate nucleus . PMID: 26724374
    2. the OX2 receptor has a critical role in modulating reward circuit in the Ventral Tegmental Area and Nucleus Accumbens PMID: 26494513
    3. Activation of orexin perifornical hypothalamus-rostral ventrolateral medulla (RVLM) neurons and orexin type 2 receptors in the RVLM facilitates epinephrine release by increasing sympathetic drive to adrenal chromaffin cells during hypoglycemia. PMID: 26653571
    4. Orexin 2 receptor (and orexin 1 receptor) contribute to the cardiovascular and locomotor response to stress. PMID: 25239810
    5. Orexins modifiy orexin receptor gene expression and gonadotropin release from the anterior pituitary gland. PMID: 24333629
    6. Elevated OX2R-mediated activity in the brain. PMID: 23525245
    7. Suggest a more prominent role for OX(2)R in ethanol self-administration compared to cue-conditioned ethanol-seeking. PMID: 23601187
    8. siRNA knockdown of OX2R in pontomesencephalic tegmentum increases duration of REM episodes. PMID: 23282008
    9. OX2R protein and mRNA are expressed in C6 glioma cells. PMID: 22588980
    10. In cultured rat cortical neurons, acting at OX2 receptors coupled to Gi protein, orexin inhibits cyclic AMP synthesis. PMID: 21547533
    11. These results demonstrate that orexins excite the cerebellar IN neurons through OX(2)R and suggest that the central orexinergic nervous system may actively participate in motor control through its modulation of the final outputs of the spinocerebellum PMID: 19921532
    12. Examine the role of hcrtR2 in nicotine's reinforcing effects. PMID: 20177882
    13. In developing hypoglossal motoneurons dissociated from 4-to 33-day-old rats, the number of mRNA copies of the orexin type 2 receptor identified per cell is significantly higher around day 20 postnatally than at any other age. PMID: 11930155
    14. Orexin Stimulation of amylase release by a pancreatic cell line is mediated by Orexin 2 receptors. PMID: 12409837
    15. Two distinct clusters of neurons containing orexin-B immunoreactivity within the amygdaloid complex may serve as a component of neuronal circuits involved in integrating complex homeostatic mechanisms associated with behaviors in the rat. PMID: 12650973
    16. Deterioration of the orexin system may play a role in the phenomenon associated with aging. PMID: 14749141
    17. Hypocretin, via the Hcrtr2 receptor, affects a specific subset of somatosensory cortex layer 6b neurons which, through corticocortical projections, may also be involved in promoting widespread cortical activation. PMID: 15282280
    18. hypocretins have roles in driving drug seeking through activation of stress pathways in the brain PMID: 16357203
    19. Thwe Ox2R signaling pathway was studied in rat olfactory mucosa and olfactory afferent neurons. PMID: 17292491
    20. Data suggest that muscarininc-2 and orexin-2 receptors may determine behavioral and state-regulating roles of GABA- and non-GABAergic neurons of the mesopontine reticular formation and lateral tegmentum and their dysfunction in narcolepsy with cataplexy. PMID: 18709662
    21. These results support a dual role for oxB/hcrt-2 in mediating glutamatergic transmission in the VTA, and suggest that oxA/hcrt-1 and oxB/hcrt-2 exert different roles in enhancing the motivational components of arousal and feeding. PMID: 18793323
    22. Biochemical and behavioural characterization of EMPA, a novel high-affinity, selective antagonist for the OX(2) receptor. PMID: 19751316
    23. Orexin receptor expression was studies in various tissues. OX2R is sexually dimorphic expressed in adrenal glands. PMID: 11459774

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed in the brain in the cerebral cortex, septal nuclei, hippocampus, medial thalamic groups, dorsal and median raphe nuclei, and many hypothalamic nuclei including the tuberomammillary nucleus, dorsomedial hypothalamus, paraventricular hypothalamic
  • 数据库链接：

    KEGG: rno:25605

    STRING: 10116.ENSRNOP00000015824

    UniGene: Rn.9893