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  4. Recombinant Rat Muscarinic acetylcholine receptor M4 (Chrm4)

Recombinant Rat Muscarinic acetylcholine receptor M4 (Chrm4)

  • 货号：
    CSB-CF005384RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Chrm4; Chrm-4; Muscarinic acetylcholine receptor M4
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-478
  • 氨基酸序列
    MXNFTPVNGSSANQSVRLVTAAHNHLETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNR QLQTVNNYFLFSLGCADLIIGAFSMNLYTLYIIKGYWPLGAVVCDLWLALDYVVSNASVM NLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVP DNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKT LAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPPPALPPPPRPVPDKDTSNESSSGSA TQNTKERPPTELSTAEATTPALPAPTLQPRTLNPASKWSKIQIVTKQTGNECVTAIEIVP ATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVL VNTFCQSCIPERVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
  • 基因功能参考文献：
    1. M1 and M4 muscarinic receptors are involved in shock-limiting and shock-activating processes, respectively, on the course of posthemorrhagic abnormalities in systemic and hepatic portal circulation. PMID: 22803069
    2. Muscarinic receptor expression is developmentally regulated; M1, M3, and M4 receptors were the main subtypes expressed in oligodendrocyte progenitor cells. PMID: 21913336
    3. M4 muscarinic acetylcholine receptors are up-regulated in dorsal root ganglion neurons and may account for increased muscarinic analgesic effects in a diabetic neuropathic pain model. PMID: 21883220
    4. CaMKII binds and phosphorylates M4 upon Ca2thorn influx to augment the antagonistic action of M4 on D1 signalling and on D1-mediated, cocaine-triggered hyperlocomotion. PMID: 20551968
    5. In a Ca(2+)-sensitive manner, CaMKIIalpha regulates M4 receptor efficacy and controls the acetylcholine-dopamine balance in the basal ganglia and also the dynamics of movement. PMID: 20461055
    6. novel role for M4 (Chrm4) in the regulation of protein synthesis through its interaction with eEF1A2. PMID: 12048193
    7. The m1-m5 muscarinic subtypes mapped to the following chromosomes: Chrm1, chromosome 1; Chrm2, chromosome 4; Chrm3, chromosome 17; Chrm4, chromosome 3; and Chrm5, chromosome 3. PMID: 12433399
    8. There is a significant reduction in the level of muscarinic M4 receptors in the spinal cord of acute and chronic arthritic rats compared to controls, suggesting that the M4 receptor subtype may be involved in cholinergic mechanisms of analgesia. PMID: 12915263
    9. These results suggest that a diet high in n-6 PUFA, but not of n-3 PUFAs or saturated fat, may selectively alter M2/M4 receptor-mediated signal transduction in the rat brain. PMID: 15927789
    10. Transient forebrain ischemia leads to dynamic alterations in the gene expression, protein prevalence, and functionality of muscarinic receptors in the post-ischemic hippocampus at times preceding the degeneration of the vulnerable neurons. PMID: 15951192
    11. evidence presented that stimulation of the muscarinic M4 receptor activates the pro-survival PI3K/Akt pathway to induce tuberin phosphorylation PMID: 15979279
    12. Data show that M1 muscarinic receptors contribute to, whereas M4 receptors inhibit, dopamine D1 receptor-induced [3H]-cyclic AMP accumulation in rat striatal slices. PMID: 16758365
    13. M4-type muscarinic receptors do not support behavioral flexibility. PMID: 17709264
    14. No m4-immunolabeling was detected in the rostral part of the thalamus. PMID: 17845913
    15. investigation of participation of M(4) receptors in neurotransmission at the hippocampal Schaffer collaterals-CA1 synapses; findings support permissive role of M(4) receptors & suggest their necessary participation in synaptic plasticity at these synapses PMID: 18816796
    16. Receptor, Muscarinic M4 activated mTOR protein and its downstream molecule Ribosomal Protein S6 Kinases (70-kDa) in a 1-Phosphatidylinositol 3-Kinase-dependent manner. PMID: 19070673

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM4 sub-subfamily
  • 数据库链接：

    KEGG: rno:25111

    STRING: 10116.ENSRNOP00000023586

    UniGene: Rn.10676