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  4. Recombinant Rat Muscarinic acetylcholine receptor M2 (Chrm2)

Recombinant Rat Muscarinic acetylcholine receptor M2 (Chrm2)

  • 货号：
    CSB-CF005382RA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Chrm2; Chrm-2; Muscarinic acetylcholine receptor M2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-466
  • 氨基酸序列
    MNNSTNSSNNGLAITSPYKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNY FLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFD RYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQF FSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKEKKEPVANQDPVSPSLVQGRI VKPNNNNMPGGDGGLEHNKIQNGKAPRDGVTENCVQGEEKESSNDSTSVSAVASNMRDDE ITQDENTVSTSLGHSRDDNSKQTCIKIVTKAQKGDVCTPTSTTVELVGSSGQNGDEKQNI VARKIVKMTKQPAKKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNT VWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
  • 基因功能参考文献：
    1. Data suggest that levels of CHRM2 are lower in Brodmann's area 24 from subjects with mood disorders, and it is possible that signalling by that receptor is also less in this cortical region; increasing levels of CHRM2 may be involved in the mechanisms of action of mood stabilisers and tricyclic antidepressants PMID: 26475745
    2. Long-term stimulation with autoantibodies against M2 muscarinic receptor leaded to the ventricular dilatation and gradual deterioration of cardiac dysfunction. PMID: 26086781
    3. The expression of alpha-SMA mRNA in detrusor muscle cells was 200 times higher than that in urothelial cells in controls. PMID: 25681120
    4. This study demonistrated that brain cholinergic receptors and acetylcholinesterase activity in rats following early life arsenic exposure. PMID: 24517892
    5. Hyperglycemia caused a higher expression of M2-muscarinic receptors in cardiomyocytes. PMID: 22293779
    6. the present study showed the involvement of M2 cholinergic receptors in the complex mechanisms of functional interactions between dopaminergic and cholinergic systems involved in the control of yawning and genital grooming. PMID: 23041487
    7. The extended distribution of M4 receptors in the cortex compared to the weaker and restricted area of distribution of M2 receptors is in favor of a major modulatory role of M4 receptors. PMID: 20600670
    8. The muscarinic M(4) receptor is the functionally predominant subtype in rat striatum. PMID: 21114972
    9. We found a tendency in the gene expression of muscarinic receptor subtypes from M2 toward M3 after birth trauma. PMID: 20445960
    10. Bile acid-induced arrhythmia is mediated by muscarinic M2 receptors in neonatal rat cardi PMID: 20300620
    11. Coexpression of GABA B2 receptors rescues cell surface expression and function of muscarinic M2 receptors at the plasma membrane. PMID: 20016095
    12. The dysfunction of inhibitory neuronal M2 muscarinic receptors in the airways protects against hyperreactivity and in the ileum contributes to gastrointestinal dysmotility associated with streptozotocin-induced diabetes. PMID: 11906948
    13. Vitamin A deficiency promotes bronchial hyperreactivity in rats by altering muscarinic M(2) receptor function. PMID: 11943668
    14. in normal bladders both M(2) and M(3) receptors can induce contraction and in the denervated bladder, the M(2) and the M(3) receptors interact in a facilitatory manner to mediate contraction. PMID: 12185001
    15. The presence of large, cholinergic C-bouton nerve terminals is associated with m2-like immunoreactivity in the postsynaptic membrane. PMID: 12717708
    16. These results show that activation of peripheral M2 receptors produces antinociception in vivo and the inhibition of nociceptor activity in vitro. PMID: 14715385
    17. evidence for the presence of M(2)- and M(3)-mAChR, at the mRNA and protein level, in the rat myometrium and that estrogen induces an increase in myometrial responsiveness to mAChR agonists PMID: 15062561
    18. the decrease of m2R in the somato-dendritic field of the the nucleus basalis magnocellularis. PMID: 15748786
    19. spontaneous contractions in the neonatal rat bladder are enhanced by activation of M2 and M3 receptors PMID: 16709645
    20. M2-like muscarinic receptor couples to a PTX-insensitive G-protein and to an ATP-dependent pathway to enhance IKV. PMID: 16953191
    21. SET protein directly interacts with the third intracellular loop of the M2 muscarinic receptor and co-immunoprecipitates with the intact receptor expressed in cells. PMID: 17065150
    22. In reticular thalamic nucleus, the m2-immunolabeling was preferentially distributed in the distal dendrite region where cholinergic afferent fibers tend to terminate and in the peripheral region of somata. PMID: 17845913
    23. Our results show that only the M(2) subtype is present in the rat atria PMID: 18443764
    24. The muscarinic receptors in the rat gastric mucosal segments were composed of M(1), M(2) and M(3) subtypes PMID: 18938154

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM2 sub-subfamily
  • 数据库链接：

    KEGG: rno:81645

    STRING: 10116.ENSRNOP00000065178

    UniGene: Rn.10752