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Recombinant Rat Melanopsin (Opn4)

In Stock
  • 货号：
    CSB-CF851271RA
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    53.9 kDa
  • 表达区域：
    1-474aa
  • 氨基酸序列
    MNSPSESRVPSSLTQDPSFTASPALLQGIWNSTQNISVRVQLLSVSPTTPGLQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCRNRGLRTPANMLIINLAVSDFLMSFTQAPVFFASSLYKKWLFGETGCKFYAFCGAVFGIVSMITLTAIAMDRYLVITRPLATIGMRSKRRTALVLLGVWLYALAWSLPPFFGWSAYVPEGLLTSCSWDYVTFTPLVRAYTMLLFCFVFFLPLLIIIFCYIFIFRAIRETGRACEGCGESPLRRRQWQRLQSEWKMAKVALIVILLFVLSWAPYSTVALVGFAGYSHILTPYMSSVPAVIAKASAIHNPIIYAITHPKYRAAIAQHLPCLGVLLGVSGQRSHPSLSYRSTHRSTLSSQSSDLSWISGQKRQESLGSESEVGWTDTETTAAWGAAQQASGQSFCSHDLEDGEVKAPSSPQEQKSKTPKTKRHLPSLDRRM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Photoreceptor that binds cis-retinaldehydes. Contributes to pupillar reflex, photoentrainment and other non-image forming responses to light. May be involved in the optokinetic visual tracking response. May be involved in the regulation of retinal hyaloid vessel growth and regression.
  • 基因功能参考文献：
    1. during retinal degeneration, the expression of melanopsin was significantly decreased, while vector-mediated overexpression of melanopsin delayed the loss of visual function in rats. PMID: 26572076
    2. Following ON injury or retrograde tracing there is a transient melanopsin downregulation that should be taken into account when assessing m(+)RGC survival. PMID: 26176868
    3. Data indicate that the phosphorylation status of melanopsin influences its functions. PMID: 25378407
    4. Early light exposure can increase the number of melanopsin immunopositive retinal ganalion cells through a process dependent on intrinsic photosensitive spiking activity. PMID: 23541830
    5. We found that light and darkness are important for regulation of melanopsin protein expression whereas input from a retinal networks regulates melanopsin mRNA expression. PMID: 23187103
    6. overexpression of mir133b decreased TH and D2 receptor expression as well as dopamine production, which finally resulted in reduced melanopsin expression PMID: 22101014
    7. Melanopsin expression defines a subset of RGCs that play a broad role in regulation of nonvisual photoreception, providing projections that contribute to circadian entrainment, negative masking, regulation of sleep-wake states, and the pupillary reflex PMID: 12904470
    8. Melanopsin, in itself, is not necessary for circadian photoreception. In fact, it appears that of the known photoreceptor systems, none, in and of itself, is necessary for circadian photoreception. PMID: 14500998
    9. During rat retinal development melanopsin is expressed at prenatal day 18 in cells of the inner neuroblast layer. and During early postnatal development a melanopsin immunoreactive dendritic network is formed in the inner plexiform layer. PMID: 15073514
    10. We investigated the effect of photoreceptor degeneration on melanopsin mRNA regulation in RCS/N-rdy rats (Royal College of Surgeons rats with a defect in the retinal dystrophy gene). PMID: 15509757
    11. Data suggest that retinal ganglion cells adapt their expression of the putative circadian photopigment melanopsin to environmental light and darkness. PMID: 16186625
    12. Regulation of melanopsin expression [review] PMID: 16687290
    13. melanopsin-containing RGCs have synaptic contact with PKC/CtBP2-containing rod bipolar cells PMID: 17652756
    14. the daily and photoperiod-dependent regulation of the melanopsin gene does not rely on a circadian oscillator but is directly illumination-dependent PMID: 18001324
    15. The PI3 K/Akt pathway was found to play a role in maintaining the survival of melanopsin-expressing retinal ganglion cells after injury. PMID: 18512147

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Perikaryon.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Opsin subfamily
  • 组织特异性：
    Eye; expressed in a photosensitive subset of retinal ganglion cells (at protein level).
  • 数据库链接：

    KEGG: rno:192223

    STRING: 10116.ENSRNOP00000015642

    UniGene: Rn.54303