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  4. Recombinant Mouse Vang-like protein 2 (Vangl2)

Recombinant Mouse Vang-like protein 2 (Vangl2)

  • 中文名称：
    小鼠Vangl2重组蛋白
  • 货号：
    CSB-CF849690MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Vangl2; Lpp1; Ltap; Vang-like protein 2; Loop-tail protein 1; Loop-tail-associated protein; Van Gogh-like protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-521
  • 氨基酸序列
    MDTESQYSGYSYKSGHSRSSRKHRDRRDRHRSKSRDGSRGDKSVTIQAPGEPLLDNESTR GDERDDNWGETTTVVTGTSEHSISHDDLTRIAKDMEDSVPLDCSRHLGVAAGAILALLSF LTPLAFLLLPPLLWREELEPCGTACEGLFISVAFKLLILLLGSWALFFRRPKASLPRVFV LRALLMVLVFLLVISYWLFYGVRILDARERSYQGVVQFAVSLVDALLFVHYLAVVLLELR QLQPQFTLKVVRSTDGASRFYNVGHLSIQRVAVWILEKYYHDFPVYNPALLNLPKSVLAK KVSGFKVYSLGEENSTNNSTGQSRAVIAAAARRRDNSHNEYYYEEAEHERRVRKRRARLV VAVEEAFTHIKRLQEEEQKNPREVMDPREAAQAIFASMARAMQKYLRTTKQQPYHTMESI LQHLEFCITHDMTPKAFLERYLAAGPTIQYHKERWLAKQWTLVSEEPVTNGLKDGIVFLL KRQDFSLVVSTKKVPFFKLSEEFVDPKSHKFVMRLQSETSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear.
  • 基因功能参考文献：
    1. a novel chemically induced mutant allele at Vangl2 called Curly Bob that causes a missense mutation p.Ile268Asn (I268N) in the Vangl2 protein, is reported. PMID: 29063958
    2. disruption of Vangl2-dependent planar-polarised processes in the posterior neuropore neuroepithelium and surface ectoderm preclude zippering point biomechanical accommodation associated with Closure 5 formation at the completion of posterior neuropore closure. PMID: 29590636
    3. The Vangl2 phosphorylation, which was previously identified to be induced by Wnt5a signaling, is required for Vangl2 functions in mammalian Planar cell polarity (PCP) in multiple tissues. PMID: 29056748
    4. type II spiral ganglion neurons make a distinctive 90 degrees turn towards the cochlear base to synapse with 10-15 outer hair cells. this axon turning event requires planar cell polarity signaling and is disrupted in Vangl2 and Celsr1 knockout mice and VANGL2 acts non-autonomously from the cochlea to direct turning. PMID: 29784671
    5. In Pax2-Cre; V1V2 Conditional knockout mice, the loss of both VANGL proteins significantly disrupts planar polarity throughout the utricular maculae PMID: 29510119
    6. The Celsr3-mediated signaling pathway responds to the inhibitory function of Wnt5a in glutamatergic synapse formation. We also revealed the opposing roles of Wnt-regulated planar cell polarity components, Celsr3 and Vangl2, in glutamatergic synapse formation. PMID: 28057866
    7. Exogenously introduced expression of green fluorescent protein (GFP)-tagged core PCP factors by electroporation revealed that Vangl1, Vangl2 and Prickle2 are localized on the ovarian side of the cell periphery in the oviduct.Our data collectively suggest that the mechanism for maintenance of Vangl2 asymmetry in mature mouse oviduct is different from the microtubule dependent polarized transport model PMID: 27155041
    8. heterozygous Looptail (Lp) mice, in which a single copy of the core PCP gene, Vangl2, is disrupted are viable but display severe airspace enlargement and impaired adult lung function. PMID: 28237967
    9. interacts with Celsr1 in ureteric tree growth PMID: 27597235
    10. Vangl2 is expressed in the inner dental epithelium (IDE) and in the secondary enamel knots (SEKs) of bell stage tooth germs. PMID: 27590885
    11. a new and essential role for Vangl2-dependent planar cell polarity signaling in the intraretinal path-finding of retinal ganglion cell axons PMID: 25990804
    12. Vangl2-regulated polarisation and subsequent acquisition of an epithelial phenotype is essential to lengthen the tubular outflow vessel, a process that is essential for on-going cardiac morphogenesis. PMID: 25521757
    13. Vangl2 regulates E-cadherin in epithelial cells. PMID: 25373475
    14. Vangl2 function in podocytes is important for glomerular development and protects against glomerular injury in adult animals. PMID: 25145929
    15. Vangl1 and Vangl2 may play a key role in the embryo implantation of mice PMID: 25445275
    16. The first signal, controlled by cadherin, EGF-like, laminin G-like, seven-pass, G-type receptor (Celsr) 2, Celsr3, Frizzled3 (Fzd3) and Van Gogh like2 (Vangl2) organizes multicilia in individual cells (single-cell polarity) PMID: 25024228
    17. Vangl does not have a critical role in neural crest migration through planar cell polarity signaling PMID: 25038043
    18. Celsr2-3 and Fzd3 regulate axonal navigation in the forebrain by using mechanisms different from classical epithelial core planar cell polarity, and require interacting partners other than Vangl1-2 that remain to be identified. PMID: 25002511
    19. Testin interacts with vangl2 genetically to regulate inner ear sensory cell orientation and the normal development of the female reproductive tract in mice. PMID: 23996638
    20. Apical boundary rearrangement is inhibited in Vangl2 mutant neural epithelium. PMID: 24703875
    21. Morphological and histological examination of homozygous mouse embryos revealed a neural tube closure defect that leads to craniorachischis ...Vangl2(m1Yzcm) may be a loss-of-function mutant. PMID: 23359061
    22. Changed auditory hair cell planar polarity in the Vangl2 KO mice leads to hearing deficits. PMID: 23986237
    23. Data indicate that Sestd1 cooperates with Dact1 in Vangl2 regulation and in the planar cell polarity (PCP) pathway during mammalian embryonic development. PMID: 23696638
    24. Sdc4 has a dynamic expression pattern during development and is detected in tissues, including the neural tube during and following closure, the hindgut and the cochlea. Co-expression with Vangl2 is consistent with a possible role for Sdc4 in Wnt/PCP signaling during morphogenesis. PMID: 23760952
    25. Vangl2 is a new component of the PSD that forms a complex with PSD-95 in the adult brain. PMID: 23567299
    26. Cofilin and Vangl2 cooperate in the initiation of planar cell polarity in the mouse embryo. PMID: 23406901
    27. Ryk regulates Wnt5a signaling and planar cell polarity by modulating the degradation of Vangl2. PMID: 23144463
    28. A point mutation within the C-terminal Vangl2 region that leads to profound planar cell polarity neural tube defects. PMID: 23029439
    29. findings identify Gipc1 as a new interactor for Vangl2; show that a myosin VI-Gipc1 protein complex can regulate Vangl2 traffic in heterologous cells; results indicate the strong asymmetry of Vangl2 observed in early postnatal cochlear epithelium is mostly a 'tissue' polarity readout PMID: 22991442
    30. loss of Dvl2 function in Vangl2(Lp/+) embryos did not exacerbate the Vangl2(Lp/+) neuronal migration phenotype PMID: 22771245
    31. A vangl2 knockout mouse was compared for the severity of the knockout and Looptail mutant phenotypes. PMID: 22363783
    32. These results identify nitric oxide as a key messenger in satellite cells maintenance and expand the significance of the Vangl2-dependent Wnt noncanonical pathway in myogenesis. PMID: 22084027
    33. A novel hypomorphic Looptail allele at the planar cell polarity Vangl2 gene. PMID: 21404367
    34. Vangl2 is required for normal kidney morphogenesis of both the ureteric bud and metanephric mesenchyme-derived structures, VANGL mutations should especially be sought in humans who are found to have both a neural tube defect and a malformed renal tract PMID: 20843830
    35. Wnt5a signaling gradient controls limb elongation by establishing PCP in chondrocytes along the proximal-distal axis through regulating Vangl2 phosphorylation. PMID: 21316585
    36. The antagonistic functions of Vangl2 and Dvl1 allow sharpening of planar cell polarity signaling locally on the tips of the filopodia to sense directional cues, Wnts, eventually causing turning of growth cones. PMID: 21316586
    37. Vangl2 transcript downregulation might be implicated in the occurrence of mouse NTDs induced by retinoic acid. PMID: 19012162
    38. Data propose that Vangl2 affects cell adhesion and the cytoskeleton by recruiting Rac1 and targeting its activity in the cell to adherens junctions. PMID: 20067994
    39. Data present evidence that Sec24b deficiency specifically affects transport of the planar cell polarity core protein Vangl2, based on experiments in embryos and in cultured primary cells. PMID: 20215345
    40. mutation in Vangl2 results in significant disruptions in the polarization of stereociliary bundles in mouse cochlea due to defects in the direction of movement and/or anchoring of the kinocilium within each hair cell PMID: 12724779
    41. Vangl2 physically interacts with the three members of the cytoplasmic Dishevelled (Dvl) protein family. PMID: 15456783
    42. Together, our data support the idea that protein asymmetry plays an important role in the development of PCP, but the colocalization and interaction of Fz3 and Vangl2 suggests that novel PCP mechanisms exist in vertebrates. PMID: 16687519
    43. Examination of Vangl2 localization in the neural tubes and cochleas of the normal and Loop-tail, Lp/Lp embryos shows disruption of normal membrane localization of Vangl2 in independent alleles at Lp as well as overall decrease in the expression level. PMID: 16962386
    44. gene mutations led to defective convergent extension in both axial mesoderm and neuroepithelium, before the onset of neurulation PMID: 17229766
    45. double heterozygosity for mutations in both Scrib and Vangl2 can cause cardiac defects similar to those found in homozygous mutants for each gene but without other major defects. PMID: 17556662
    46. These data suggest that Vangl2 and Rho kinase act cell autonomously in the myocardium to regulate the organization of cardiomyocytes but also have non-cell-autonomous effects on the formation of the coronary vasculature. PMID: 18174466
    47. Genetic interaction between Vangl1 and Vangl2 genes causes neural tube defects. Vangl1(gt/+);Vangl2(lp/+) double heterozygotes show profound developmental defects PMID: 18296642
    48. Stbm/Vangl2 functions to maintain cortical progenitors and regulates mitotic spindle orientation during asymmetric divisions. PMID: 19332887
    49. Vangl2(Lp) mutant uterine epithelium displays altered cell polarity, concommitant with changes in cytoskeletal actin and scribble (scribbled, Scrb1) localization PMID: 19363157
    50. Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. PMID: 19701191

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  • 相关疾病：
    Defects in Vangl2 are a cause of the loop-tail (Lp) mutant phenotype. Heterozygous Lp mice exhibit a characteristic looped tail, while homozygous embryos show a completely open neural tube in the hindbrain and spinal region, a condition similar to the severe craniorachischisis defect in humans. Homozygotes also have complex cardiovascular defects including double-outlet right ventricle, perimembranous ventricular defects, double-sided aortic arch and associated abnormalities in the aortic arch arteries. Homozygotes show cytoplasmic accumulation of Vangl2 instead of the normal membrane localization, and Rhoa expression, which is detected in the mesenchymal cushion cells adjacent to the outflow tract, is lost in homozygotes. Homozygous embryos typically die shortly before or at birth.
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Vang family
  • 组织特异性：
    Primarily expressed in the brain and epididymis. Not detected in the cochlea of Lp mice.
  • 数据库链接：

    KEGG: mmu:93840

    STRING: 10090.ENSMUSP00000027837

    UniGene: Mm.36148