Recombinant Mouse Synaptotagmin-1 (Syt1)

1. Mutating Ca(2+)-coordinating aspartates in the C2A-domain localizes Doc2B permanently at the plasma membrane, and renders an upstream priming step Ca(2+)-independent, whereas a separate function in downstream priming depends on SNARE-binding, Ca(2+)-binding to the C2B-domain of Doc2B, interaction with ubMunc13-2 and the presence of synaptotagmin-1. PMID: 29274147
2. Study showed postsynaptic localization of synaptotagmin 1, at concentrations moderately lower than, but comparable to presynaptic concentrations. It is present in significant concentrations at the postsynaptic density, pointing to the likelihood of insertion of glutamate receptors directly into the synaptic plasma membrane. Synaptotagmin 1 is reduced in postsynaptic spines after eight weeks of kainite-induced epilepsy. PMID: 28686803
3. that reduction in the synaptotagmin 1 level and presenilin 1-synaptotagmin 1 interactions in AD brain may present molecular underpinning of the pathogenic presenilin 1 conformation PMID: 28193235
4. s propose that the strong reduction of Syt2 and SV2B are key factors of the functional synaptic alteration and that the physiological downregulation of Syt1 plays a determinant role in muscle vulnerability in SMA. PMID: 28173138
5. we identify Syt2 as a functionally important Ca(2+) sensor at fast-releasing inhibitory synapses, and show that Syt1 and Syt2 can redundantly control transmitter release at specific brain synapses PMID: 28363983
6. results suggest that postsynaptic Syt1 and Syt7 act as redundant Ca(2+)-sensors for Ca(2+)-dependent exocytosis of AMPA receptors during long-term potentiation, and thereby delineate a simple mechanism for the recruitment of AMPA receptors that mediates LTP PMID: 28355182
7. demonstrates a developmental Syt1-Syt2 isoform switch at an identified synapse, a mechanism that could fine-tune the speed, reliability, and plasticity of transmitter release at fast releasing CNS synapses. PMID: 27210552
8. the combined inactivation of all 3 E-Syt genes has no effect on mouse viability or fertility. PMID: 27399837
9. We conclude that synaptotagmin-1 phosphorylation is an essential step in PKC-dependent potentiation of synaptic transmission, acting downstream of the two other essential DAG/PKC substrates, Munc13-1 and Munc18-1. PMID: 27091977
10. data show that hepatic Syt1 expression is influenced by diet and hormonal milieu PMID: 26709651
11. different structural states of syt underlie the control of distinct forms of synaptic transmission. PMID: 27001899
12. The interaction of Dvl1 with Syt-1, which is regulated by Wnts, modulates neurotransmitter release. PMID: 26400647
13. Data (including data from studies in knockout mice) suggest that loss of both Syt1/Syt7 decreases capacity of readily-releasable pool (RRP) of Ca2+ in synaptic vesicles without altering rate of priming into RRP; Syt1/Syt7 functions appear redundant. PMID: 26437117
14. Syt-1 regulates Abeta levels in mouse neurons. Knockdown of endogenous Syt-1 in mouse primary neurons led to a significant reduction in both Abeta40 and Abeta42 generation. PMID: 26202512
15. Increased expression of Syt1 in neural stem cells is essential for neurogenesis progression. PMID: 24973144
16. This study provides new insights in how the two opposite sides of the C2B domain of Synaptotagmin-1 participate in secretory vesicle fusion, and in more upstream steps, especially vesicle docking. PMID: 26490858
17. atomic-resolution crystal structures of Ca(2+)- and Mg(2+)-bound complexes between synaptotagmin-1 and the neuronal SNARE complex PMID: 26280336
18. the Ca(2+) dependence of the interaction between PRIP-C2 and Syt1-C2A was attributed to Ca(2+) binding with Syt1-C2A, but not PRIP-C2, using a series of mutants prepared from both C2 domains. PMID: 25242442
19. Tissue-specific splicing, posttranslational modification and the variation in expression might suggest a different requirement of SYT1 for the specific function in each organ. PMID: 25735570
20. This study reveals fundamental details of how phosphorylation of Thr84 on SV2A controls its interaction with synaptotagmin-1 and implicates SV2A as a phospho-dependent chaperone PMID: 25673844
21. The syt function is markedly affected by changes in the physical nature of the linker that connects its tandem C2 domains. Moreover, the linker mutations uncoupled syt-mediated regulation of evoked and spontaneous release. PMID: 24657966
22. In mixed glutamatergic/GABAergic neurons, excitatory postsynaptic potential (EPSP) frequencies were increased in synaptotagmin-1-null neurons. PMID: 24501351
23. Ca(2+)-triggered release at a synapse is due to synaptotagmins, with synaptotagmin-7 mediating a slower form of Ca(2+)-triggered release that is normally occluded by faster synaptotagmin-1-induced release but becomes manifest upon synaptotagmin-1 deletion PMID: 24267651
24. Syt-1 SNARE-interacting domains are essential for vesicle docking, priming, and fast fusion-triggering in chromaffin cells. PMID: 24005294
25. Synaptotagmins 1 and 2 as mediators of rapid exocytosis at nerve terminals: the dyad hypothesis PMID: 23648184
26. APP interacts with the calcium sensor synaptotagmin-1 of synaptic vesicles and might thus play a role in the regulation of synaptic vesicle exocytosis. PMID: 22731840
27. Data suggest that Syt1 may act as a MTOC-associated protein to play important roles in mouse oocyte spindle organization/stability, and that it is indispensable for the metaphase-anaphase transition to promote mouse oocyte meiotic maturation. PMID: 22313732
28. The dynamics of vesicle fission during clathrin-mediated endocytosis is Ca(2)+ dependent but becomes Ca(2)+ independent in synaptotagmin 1 knock-out cells. PMID: 22423098
29. This study demonistrated that syt1 functions as a dual Ca(2+) sensor for both endo- and exocytosis, potentially coupling these two components of the vesicle cycle. PMID: 22197832
30. This system recapitulates some of the effects of syt mutations that alter synaptic transmission in neurons. It demonstrate that the cytoplasmic domain of syt exhibited mixed agonist/antagonist activity during regulated membrane fusion in cells. PMID: 22184197
31. this study demonistrated that Alcohol induces synaptotagmin 1 expression in neurons via activation of heat shock factor 1 PMID: 21816209
32. A test of Syt1 exocytosis in otoferlin-deficient hippocampal neurons and Syt1-deficient inner hair cells finds no deficits in vesicle fusion. PMID: 21451027
33. new-synthesized synaptotagmin1 following synaptic plasticity may contribute to long-lasting synaptic plasticity via positive, feedfoward mechanisms PMID: 21129439
34. The Syt7 C2B domain structure is very similar to that of the Syt1 C2B domain and contains three Ca2+-binding sites. PMID: 20824061
35. Transient expression of Syt1 is no longer detected in cochlear inner hair cells (IHCs) after the onset of hearing, indicating that the most common calcium-ion sensors in the central nervous system are not involved in the mature IHC ribbon synapse. PMID: 20926654
36. Expression of Syt1 in the mouse prefrontal cortex is modulated by a sequence variant (B2 SINE indel) in the 3' UTR of Comt (catechol-O-methyltransferase). PMID: 20808911
37. 1.9-A X-ray structure of the BoNT serotype G receptor binding domain on the protein receptor synaptotagmin (Syt) I/II. PMID: 20219474
38. intestinal epithelial Syt 1 plays an important role in cAMP-stimulated endocytosis of apical NHE3 through cAMP-dependent phosphorylation of S605 that is required for NHE3 and Syt 1 association PMID: 19926819
39. synaptotagmin-I expressing neuroblastoma cells require gangliosides for Botulinum neurotoxin A activity PMID: 12089155
40. In synaptotagmin 1 knockout mice, the fast component of both Ca(2+)- and Sr(2+)-induced neurotransmitter release is impaired, suggesting that both cations partly act by binding to synaptotagmin 1. PMID: 12526776
41. Ca2+-dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca2+ dependence of exocytosis. PMID: 12939392
42. syts I and II can function as protein receptors for Botulinum neurotoxin b and mediate its entry PMID: 14504267
43. alternative translation of synaptotagmin 1 mediates the non-classical release of fibroblast growth factor 1 PMID: 14559220
44. Synaptotagmins I and II are nerve cell receptors for botulinum neurotoxin G PMID: 15123599
45. Synaptotagmin I synchronizes the rapid release of neurotransmitters after Ca2+ entry into presynaptic terminals and also appears to suppress the slower, asynchronous form of transmitter release. PMID: 15240804
46. We have used point mutagenesis to determine the functional contributions to triggering synchronous neurotransmitter release of the five negatively charged aspartate residues that constitute the Ca2+-binding sites in the C2B domain of synaptotagmin I. PMID: 15456828
47. SV2B-synaptotagmin 1 complex is involved in the regulation of synaptotagmin 1 stability and/or trafficking PMID: 15466855
48. endocytosis is significantly impaired in the absence of SytI with the relative rates of endocytosis compared with exocytosis reduced approximately 3-fold with respect to wild type PMID: 15492212
49. synaptotagmin 1 function requires a free C terminus, possibly because of spatial constraints at the release sites PMID: 15561725
50. We conclude that synaptotagmin self-oligomerization is unlikely to play any significant role in triggering synaptic vesicle exocytosis. PMID: 15886015

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KEGG: mmu:20979

STRING: 10090.ENSMUSP00000063293

UniGene: Mm.289702