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  4. Recombinant Mouse Steroidogenic factor 1 (Nr5a1)

Recombinant Mouse Steroidogenic factor 1 (Nr5a1)

  • 货号：
    CSB-CF016065MO
  • 规格：
    ￥5268
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Nr5a1
  • Uniprot No.：
  • 别名：
    Nr5a1; Ftzf1; Steroidogenic factor 1; SF-1; STF-1; Adrenal 4-binding protein; Embryonal LTR-binding protein; ELP; Embryonal long terminal repeat-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP; Steroid hydroxylase positive regulator
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 表达区域：
    1-462aa
  • 氨基酸序列
    MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPPPDYMLPPSLHAPEPKALVSGPPSGPLGDFGAPSLPMAVPGPHGPLAGYLYPAFSNRTIKSEYPEPYASPPQQPGPPYSYPEPFSGGPNVPELILQLLQLEPEEDQVRARIVGCLQEPAKSRSDQPAPFSLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVLDHIYRQVQYGKEDSILLVTGQEVELSTVAVQAGSLLHSLVLRAQELVLQLHALQLDRQEFVCLKFLILFSLDVKFLNNHSLVKDAQEKANAALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
  • 基因功能参考文献：
    1. ChIP-sequence/ChIP studies indicated that Ad4BP/SF-1 binds to the upstream region of Ccne1 (cyclin E1) gene during G1/S phase. PMID: 28325912
    2. Collectively, this evidence suggests that SF-1 expression in ventromedial hypothalamic nucleus neurons is required for the beneficial effects of exercise on metabolism. PMID: 27874828
    3. Data suggest that Ad4BP plays role in regulation of intracellular NADPH concentration via transcription of Me1 and Mthfd2 genes in adrenocortical cells. (Ad4BP = nuclear receptor subfamily 5 group A member 1; Me1 = malic enzyme 1; Mthfd2 = bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase) PMID: 28202838
    4. Our data suggested that lipid accumulation in the Leydig cells of the 46,XY disorders of sex development phenotype with a steroidogenic factor 1 mutation is due, at least in part, to the suppression of steroidogenic acute regulatory protein and CYP11A1, and a resulting increase in unmetabolized cholesterol. PMID: 27455990
    5. SF-1 is needed for proper development of fetal and adult Leydig cells but with distinct primary functions PMID: 26269506
    6. NR5A1 prevents centriole splitting by inhibiting centrosomal DNA-PK activation and beta-catenin accumulation PMID: 25421435
    7. The results suggest that gonadal steroids do not influence the production of neurosteroids in the fetal brain, nor does SF-1 play a major role in the regulation of steroidogenic enzyme expression in the brain. PMID: 25111584
    8. MiR-383 promoted the expression of miR-320 which regulated granulosa cell functions by targeting E2F1 and SF-1 proteins. PMID: 24828505
    9. we provide the evidence that the acidic residue (E199) located downstream from the core consensus SUMO site of NR5A1 is, at least in part, required for SUMOylation of NR5A1 and for its mediated target gene and protein expression. PMID: 24232453
    10. GATA6 regulates the differentiation of steroidogenic (SF-1 expressing) progenitors into adrenocortical cells. PMID: 23471215
    11. SF-1 depletion induces centrosome overduplication by activating centrosomal CDK2/cyclin A. PMID: 23166296
    12. Pin1 is required for the Ser203 phosphorylation-dependent ubiquitination of SF-1, which facilitates SF-1-Pitx1 interactions and therefore results in an enhancement of SF-1 transcriptional activity PMID: 19995909
    13. data suggest a novel role for SF1 in promoting ovarian development in addition to its roles in testis differentiation PMID: 19757380
    14. SF-1 is essential for TGF-beta3-mediated 17beta-estradiol synthesis in granulosa cells PMID: 21586554
    15. Dicer1 is dispensable for formation and differentiation of fetal tissues derived from the SF1-positive adrenogonadal primordium. PMID: 20540774
    16. SF-1 expression is induced by PGC-1a in both pituitary & adrenal-cortical cells; SF-1 & PGC-1a form complex & co-localize to LHb & Cyp11b2 promoter regions; SF-1/PGC-1a coactivate LHb expression in pituitary cells PMID: 21108604
    17. SF-1 knockdown affects the accumulation of a large number of transcripts, most of which are not recognizably involved in steroid hormone biosynthesis. PMID: 21111771
    18. This study demonstrated that SUMO modification of transcription factors such as SF-1 is important in vivo and provides an essential, added layer of regulation for fine-tuning temporal and spatial gene expression in mammalian organ development. PMID: 21820362
    19. SF-1 acts as a positive regulator of miR-383 processing and function in ovarian granulosa cells PMID: 22593182
    20. NR5A1 is essential for Sertoli cell maturation and therefore spermatogenesis, during postnatal testis development. PMID: 22419830
    21. Data describe the roles of down-regulated steroidogenic factor-1 in basal and Ang II-induced CYP11B2 expression using SF-1-specific short hairpin RNA. PMID: 21169726
    22. SF-1 plays a dynamic role during the differentiation of P19 cells and potentially during early embryogenesis. PMID: 21678401
    23. Our results uncover a novel role of SF-1 in the control of centrosome homeostasis and genomic stability PMID: 21566663
    24. Ad4BP/SF-1 gene expression in the fetal Leydig cell is regulated by a yet unidentified E-box binding protein(s) and by an autoregulatory loop formed by Ad4BP/SF-1. PMID: 22128023
    25. Changes in the cytoarchitecture of the region of the ventromedial hypothalamus with respect to the distribution of immunoreactive NPY, AgRP and CART occur in SF-1 knockouts. PMID: 21906594
    26. Data indicate that SF-1 expression (followed by LIF removal and treatment with cAMP) drives embryonic stem cells into a steroidogenic pathway characteristic of gonadal steroid producing cells (i.e., de novo synthesis of estradiol/testosterone). PMID: 21610156
    27. Data show that the tissue-specific expression of SF-1/Ad4BP is epigenetically regulated and identify tissue-specific differentially methylated regions within the sf-1/ad4bp locus that are essential for its transcriptional control. PMID: 21343250
    28. These results indicated that pluripotent stem cells could be differentiated into steroidogenic cells by the nuclear receptor 5A family of protein via the mesenchymal cell lineage. PMID: 21129436
    29. Transient transfection experiments performed within developing testes and ovaries are a powerful tool to uncover elements within the Sf1 promoter that contribute to sex-specific expression. PMID: 20962249
    30. NR5A1 is expressed in ovary and adrenal gland; NR5A1 enhances the promoter activity of Star; NR5A1 and FOXL2 synergistically up-regulate the transcriptional activity of Mc2r PMID: 20650879
    31. silencing of integrated Nr5a1 constructs and the greater efficiency of transient transfection suggest that continued high expression might even be deleterious in naive pluripotent cells. PMID: 20823062
    32. SF-1 in the ventromedial hypothalamic nucleus plays crucial roles in regulation of female reproductive function. PMID: 20339005
    33. Newborn mice deficient in NR5A1 lack both gonads and adrenal glands and have impaired expression of pituitary gonadotrophins. PMID: 20394914
    34. Through a unique interaction between the SF1-positive cortical cells (source of SHH) and SF1-negative capsular cells (targets of SHH), progenitor cells in the adrenal capsule expand. PMID: 20118198
    35. FXR is able to compete with steroidogenic factor 1 in binding to a common sequence present in the aromatase promoter region interfering negatively with its activity. PMID: 20026603
    36. Basal and cyclic AMP-induced Rbp4 transcription is regulated by a multiprotein complex that is similar to ones that modulate expression of genes of steroid hormone biosynthesis. PMID: 19389484
    37. describe phenotypic spectrum of disorders associated with mutations and reveal how the discovery of naturally occurring mutations is helping to unravel the role in development and disease [review] PMID: 11738790
    38. REVIEW. Knockout mice lacking SF-1 revealed its essential roles at multiple levels of endocrine development and function. PMID: 11738791
    39. expression of endogenous RLF correlates with the amount of steroidogenic factor 1 (SF-1) expression PMID: 11861512
    40. Dax1 expression is dependent on steroidogenic factor 1 in the developing gonad PMID: 11923472
    41. Both p300/CBP/cointegrator-associated protein (p/CIP) and TIF2 potentiated SF-1-mediated transcription PMID: 11923473
    42. Differential regulation of aldosterone synthase and 11beta-hydroxylase transcription by steroidogenic factor-1. PMID: 11932209
    43. a pathway in which the products of the Wt1 and Lhx9 genes activate expression of sf1 and thus mediate early gonadogenesis PMID: 12130543
    44. Multiple cell lineages express SF-1, including steroidogenic cells of the adrenal cortex, testes, and ovaries, neurons of the ventromedial hypothalamic nucleus, and reticuloendothelial cells of the spleen. PMID: 12351700
    45. findings demonstrate that mouse neuronal nitric oxide synthase gene expression is regulated by the steroidogenic factor-1 gene family in pituitary gonadotropes PMID: 12456803
    46. Yeast two-hybrid and transfection assays confirmed the necessity of three LXXLL-related motifs in Dax-1 for interaction with and repression of Ad4BP/SF-1. In vitro pull-down experiments confirmed that Dax-1 interacts with Ad4BP/SF-1 and also with LRH-1. PMID: 12482977
    47. New type of SF-1 binding element of akr1b7 gene affecting promoter in adult mice. PMID: 12530656
    48. Mouse strains with reportedly high steroidogenic capacity also have SF1(A172) allele while mouse strains with low steroidogenic capacity have the SF1(S172) allele. PMID: 12530657
    49. New function for SF-1 response element in vivo, via influencing promoter sensibility to postnatal changes of SF-1 contents, in controlling promoter strength in adults without affecting adrenal targeting, hormonal control, or early gene expression. PMID: 12697720
    50. SF-1 mediates terminal differentiation of the ventromedial nucleus; transcriptional targets of SF-1 mediate normal circuitry between the hypothalamus and limbic structures in the telencephalon. PMID: 12727442

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR5 subfamily
  • 数据库链接：

    KEGG: mmu:26423

    STRING: 10090.ENSMUSP00000028084

    UniGene: Mm.31387