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  4. Recombinant Mouse Phosphatidylethanolamine N-methyltransferase (Pemt)

Recombinant Mouse Phosphatidylethanolamine N-methyltransferase (Pemt)

  • 中文名称：
    小鼠Pemt重组蛋白
  • 货号：
    CSB-CF017780MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Pemt
  • Uniprot No.：
  • 别名：
    Pemt; Pempt; Pemt2; Phosphatidylethanolamine N-methyltransferase; PEAMT; PEMT; Phospholipid methyltransferase; PLMT
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-199
  • 氨基酸序列
    SWLLGYMDPTEPSFVAAVITIVFNPLFWNVVARWEQRTRKLSRAFGSPHLACYSLGICIL LLNILRSHCFTQAMMSQPKMEGLDNHTTYFLGLAFLGWGFVFVLSSFYALGFTGTFLGDY FGILKESRVTTFPFSVLDNPMYWGSTANYLGWALMHASPTGLLLTVVVAIVNVVALLYEE PFTAEIYRQKATRLHKRS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC).
  • 基因功能参考文献：
    1. fenofibrate partially reversed hepatic steatosis and fibrosis in Pemt(-/-) mice when treatment was initiated after nonalcoholic fatty liver disease (NAFLD) had already been established. Increasing hepatic fatty acid oxidation can compensate for the lower VLDL-triacylglycerol secretion rate and prevent/reverse fatty liver disease in mice lacking PEMT. PMID: 28159867
    2. Results show that hepatic PEMT protein levels and activity are post-translationally repressed in homocystinuria and correlates with decreased phosphatidylcholine. PMID: 28291718
    3. Activation of PPARgamma using pioglitazone in high fat diet-fed Pemt(-/-) mice improved liver function, while these mice were still protected against diet-induced obesity and insulin resistance. PMID: 26797396
    4. Results showed that Pemt deficiency and high-fat diet in mouse model demonstrated the phenotypes resemble to the clinical features of the patients with lean non-alcoholic steatohepatitis. PMID: 26883167
    5. propose that cold-induced hypothermia in HF-fed Pemt(-/-) mice is linked to plasma hypoglycemia due to compromised hepatic glucose production. PMID: 26113536
    6. Lack of PEMT in mice does not promote fatty acid oxidation in skeletal muscle. PMID: 26603903
    7. Decreased lipogenesis in white adipose tissue may contribute to the resistance to diet-induced obesity in Pemt(-/-) mice. PMID: 25463480
    8. these findings indicate that the inhibition of Pemt activity ameliorates the ER stress associated with diabetic nephropathy in a model of type 1 diabetes PMID: 24667182
    9. This study evaluated the role of the role of phosphatidylethanolamine N-methyltransferase in hepatic carbohydrate metabolism in chow-fed mice. PMID: 24677714
    10. Pemt deficiency results in attenuated secretion of very low-density lipoproteins and homocysteine as well as in increased susceptibility to nonalcoholic liver disease. PMID: 22877991
    11. Lack of phosphatidylethanolamine N-methyltransferase decreased liver damage in Abcb4(-/-) mice caused by exposure of the liver to excess bile acids. PMID: 21292027
    12. Treatment strategies aimed at inhibition of PEMT might prevent the accumulation of cardiac triacylglycerol that predisposes individuals to compromised cardiac function. PMID: 21273556
    13. de novo synthesis of choline via PEMT has a previously unappreciated role in regulating whole body energy metabolism. PMID: 20452975
    14. During differentiation of 3T3-L1 cells to adipocytes, the amount of Sp1 protein decreased by approximately 50% just prior to activation of PEMT. PMID: 20150657
    15. Dietary docosahexaenoic acid supplementation modulates hippocampal development in the Pemt-/- mouse. PMID: 19889625
    16. PEMT is required in the secretion of apoB100-containing VLDLs. PMID: 12193594
    17. Results show that phosphatidylethanolamine N -methyltransferase (PEMT) knockout mice do not display normal concentrations of choline metabolites even with a supplemental source of dietary choline. PMID: 12466019
    18. Plasma homocysteine is regulated by phospholipid methylation, and is therefore lowered in enzyme-deficient mice. PMID: 12482759
    19. PEMT activity is involved in many physiologic processes including the flux of lipid between liver and plasma and the delivery of essential fatty acids to blood and peripheral tissues via the liver-derived lipoproteins PMID: 14608048
    20. This study observed increased numbers of phosphorylated histone H3 positive cells in the Pemt-/- mice (up 54% compared to wild-type mice; p<0.01). We also found decreased calretinin labeling in Pemt-/- (down to 43% compared to wild-type mice; p<0.01). PMID: 15063092
    21. deficiency causes severe liver damage in conjunction with choline-deficient diet PMID: 15164765
    22. Mouse PEMT genes have three unique transcription start sites, which are indicative of either multiple promoters and/or alternative splicing. PMID: 17456783
    23. We conclude that the biosynthesis of PC via the PEMT pathway is not quantitatively essential for biliary PC secretion under acute or chronic bile salt administration. PMID: 17595447
    24. Hepatic phosphatidylethanolamine N-methyltransferase has roles in animal biochemistry and physiology [review] PMID: 17881348
    25. PEMT is involved in the regulation of plasma HDL levels in mice, mainly via HDL lipid uptake by SR-BI. PMID: 18842588
    26. Lack of phosphatidylethanolamine N-methyltransferase alters plasma VLDL phospholipids and attenuates atherosclerosis in mice. PMID: 19520976

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Class VI-like SAM-binding methyltransferase superfamily, PEMT/PEM2 methyltransferase family
  • 组织特异性：
    Liver.
  • 数据库链接：

    KEGG: mmu:18618

    STRING: 10090.ENSMUSP00000000310

    UniGene: Mm.2731