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  4. Recombinant Mouse P2Y purinoceptor 12 (P2ry12)

Recombinant Mouse P2Y purinoceptor 12 (P2ry12)

  • 货号：
    CSB-CF861428MO
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    45.5 kDa
  • 表达区域：
    1-347aa
  • 氨基酸序列
    MDVPGVNTTSANTTFSPGTSTLCVRDYKITQVLFPLLYTVLFFAGLITNSLAMRIFFQIRSKSNFIIFLKNTVISDLLMILTFPFKILSDAKLGAGPLRTLVCQVTSVTFYFTMYISISFLGLITIDRYLKTTRPFKTSSPSNLLGAKILSVVIWAFMFLISLPNMILTNRRPKDKDVTKCSFLKSEFGLVWHEIVNYICQVIFWINFLIVIVCYSLITKELYRSYVRTRGSAKVPKKKVNVKVFIIIAVFFICFVPFHFARIPYTLSQTRAVFDCSAENTLFYVKESTLWLTSLNACLDPFIYFFLCKSFRNSLTSMLRCSNSTSTSGTNKKKGQEGGEPSEETPM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Required for normal platelet aggregation and blood coagulation.
  • 基因功能参考文献：
    1. OC formation was efficiently restored by addition of adenosine diphosphate, a P2Y12 agonist, as well as by addition of CysLT. Furthermore, similar to montelukast, P2Y12 blockade by a pharmacological inhibitor or siRNAs suppressed OC differentiation PMID: 29916540
    2. Our finding demonstrated that genetic deletion of P2Y12 receptor broke the balance of Th subtypes by affecting the cytokine profile of bone marrow-derived dendritic cells and resulted in the aggravated experimental autoimmune encephalomyelitis, which suggested that P2Y12 may be a potential target in treating multiple sclerosis. PMID: 27939246
    3. Study demonstrates a complementary role between TSSC6 and P2Y12 receptor in platelets in regulating 'outside in' integrin alphaIIbbeta3 signalling thrombus growth and stability. PMID: 29178985
    4. Findings offer insight into positive regulation of Akt signaling through P2Y12 phosphorylation as well as MAPK signaling in platelets by ASK1. PMID: 28753204
    5. CLEC-2 and P2Y12 are required for CpG ODN-induced platelet activation and thrombosis, and might be targeted to prevent adverse events in patients at risk. PMID: 28296036
    6. Nucleotide transmitters ATP and ADP mediate intercellular calcium wave communication via P2Y12/P2Y13 receptors among BV-2 microglia. PMID: 28800362
    7. findings not only revealed the critical role of P2Y12 in Th17 differentiation and EAE pathogenesis, but also suggested the promising potential of P2Y12 antagonists in the treatment of autoimmune diseases PMID: 28515278
    8. P2y12 Receptor Promotes Pressure Overload-Induced Cardiac Remodeling via Platelet-Driven Inflammation in Mice. PMID: 28827474
    9. In P2Y12(-/-) mice, the growth of syngeneic ovarian cancer tumors was reduced by >85% compared with wild-type (WT) mice. In contrast, there was no difference in tumor growth between P2Y1(-/-) and WT mice. PMID: 28679740
    10. Vessel wall P2Y12 receptor, which promotes VSMCs migration through cofilin dephosphorylation, plays a critical role in the development of atherosclerotic lesions. PMID: 28062501
    11. P2Y12 null mice are refractory to sepsis-induced lung injury, suggesting a key role for activated platelets and the P2Y12 receptor during sepsis. PMID: 27055904
    12. excessive mechanical stress may induce a sustained release of ADP and promote SMC senescence via P2ry12-dependent sustained Ras activation, thereby contributing to excessive inflammation and degeneration, which provides insights into TAAD formation and progression. PMID: 27534720
    13. AHR and airway inflammation in a mouse model of asthma. P2Y12 can be a novel therapeutic target to the suppression of eosinophils in asthma. PMID: 26612496
    14. quinine/bupivacaine-sensitive potassium channels are the functional effectors of the P2Y12R-mediated signaling in microglia activation following neuronal injury PMID: 26791526
    15. Calcium-mediated purinergic receptors regulate the migration and phagocytic ability of microglia during post-natal brain development. PMID: 25575683
    16. Disrupting P2Y12 alters the microglial response to monocular deprivation and abrogates ocular dominance plasticity. PMID: 26948129
    17. P2RY12-mediated activation of juxtavascular microglial cells contributes to the rapid closure of small openings in the BBB. PMID: 26755608
    18. study reports robust involvement of P2Y12R in inflammatory pain: anti-hyperalgesic effect of P2Y12R antagonism could be mediated by the inhibition of both central and peripheral cytokine production and involves alpha7-receptor mediated efferent pathways PMID: 24971933
    19. demonstrating that these P2Y12 inhibitors may not be effective in preventing early disease PMID: 24510394
    20. indicate that P2Y12R undergoes slow desensitization; ADP-P2Y12 signaling is tightly regulated by Entpd1/Nt5e activity and adenosine receptors PMID: 25208846
    21. P2Y12 signaling is required for stabilizing platelet thrombi. PMID: 24477045
    22. Platelet P2Y12 is involved in murine pulmonary metastasis. PMID: 24236201
    23. The P2Y12 receptor plays an important role during lipopolysaccharide-induced inflammation. PMID: 24142066
    24. P2Y12 participates in ischemia related inflammation by mediating microglial migration and potentiation of neurotoxicity. PMID: 23940669
    25. This study demonistrated that environment enhanced P2Y12 expression in brain and enhanced totaroad perfermance and short term working menory. PMID: 23558143
    26. Epac agonist 8-pCPT-2'-O-Me-cAMP affects P2Y12 receptors in blood platelets PMID: 23850619
    27. Demonstrate that P2Y(12) activation protects platelets from apoptosis via PI3k-dependent Bak/Bax inactivation. PMID: 23140172
    28. P2RY12 is the primary ADP receptor in osteoclasts. In vivo, P2ry12-/- mice were partially protected from pathologic bone loss, as were those with clopidogrel-induced inhibition of P2ry12. PMID: 22996695
    29. P2Y12 modulates atherogenesis, at least in part by augmenting inflammatory cell recruitment via regulation of platelet alpha-granule release. PMID: 22628428
    30. beta-arr1 has a role in promoting thrombus formation, in part through its participation in alphaIIbbeta3 signalling, but neither beta-arr1 or beta-arr2 have a role in agonist-induced platelet activation and P2Y receptors desensitisation PMID: 22318609
    31. Real-time imaging reveals that P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent. PMID: 22199395
    32. P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent. PMID: 22057273
    33. P2Y12-mediated migration of host-derived smooth muscle-like cells and CD45+ leukocytes may play an important role in the development of transplant arteriosclerosis, partly by MCP-1 pathways. PMID: 21629176
    34. data revealed that the P2Y12 receptor pathway was substantially involved in platelet hyperreactivity associated with mild and severe hypercholesterolemia PMID: 21261805
    35. Platelet P2Y(12) receptors play a crucial role in the stabilization of thrombi formed on atherosclerotic plaques. This P2Y(12) function is restricted to high shear flow conditions, and is preserved in the presence of coagulation. PMID: 20405028
    36. Studies indicate that CalDAG-GEFI mediates the rapid but reversible activation of integrin alphaIIbbeta3, and the adenosine diphosphate receptor P2Y12 facilitates delayed but sustained integrin activation PMID: 20971951
    37. Exposure to smoke heightens platelet reactivity and thrombosis in Apoe-/- mice and implicate signaling through platelet P2Y12 receptor as a mediator of the adverse consequence of smoke exposure. PMID: 20451958
    38. Ticagrelor effectively and reversibly inhibits P2Y(12)-mediated platelet function and thrombosis in mice. P2Y(12) inhibition is required both at the time of and after injury to effectively inhibit neointima formation. PMID: 21071697
    39. Data show that autocrine purinergic receptor P2Y(2), or P2Y(12) signaling amplifies and translates chemotactic cues into directional motility. PMID: 20664064
    40. P2ry12 signaling was more important than PAR signaling in hemostasis models; the converse was noted for arterial thrombosis models. PMID: 20930120
    41. Data detected mRNA and protein for ADP receptors P2Y12 and P2Y13 in mouse dorsal root ganglia (DRG). P2Y14, a homologous Gi-coupled nucleotide receptor, is also expressed in DRG. PMID: 20398327
    42. role of P2Y12 in platelet thrombosis and how its complete absence affects the thrombotic process PMID: 12897207
    43. Results describe the cloning of DNA sequences encoding the murine ortholog of the human P2Y12 receptor, and its expression in the mouse brain. PMID: 15464998
    44. secreted ADP strongly potentiated Rac activation induced by FcgammaRIIa clustering or TRAP via its P2Y12 receptor PMID: 16194206
    45. P2Y(12) is a primary site at which nucleotides act to induce microglial chemotaxis at early stages of the response to local CNS injury. PMID: 17115040
    46. the combined catalytic and non-catalytic signaling function of p110 beta and p110 gamma is critical for P2Y(12)/G(i)-dependent integrin alpha(IIb)beta(3) regulation. PMID: 17673465
    47. These results clearly provide additional in vivo evidence that prasugrel has selective P2Y(12) antagonistic activity. PMID: 17681285
    48. Activation of P2Y(12)Rs in spinal microglia may be critical event in pathogenesis of neuropathic pain. Blocking microglial P2Y(12)R might be viable therapeutic strategy for treating neuropathic pain. PMID: 18463248
    49. role for platelet P2Y(12) in the vessel wall response to arterial injury and thrombosis PMID: 19103996
    50. identified a molecular pathway in microglia that converted ATP-driven process extension into process retraction during inflammation: reversal was driven by upregulation of the A(2A) adenosine receptor coincident with P2Y(12) downregulation. PMID: 19525944

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 数据库链接：

    KEGG: mmu:70839

    STRING: 10090.ENSMUSP00000051353

    UniGene: Mm.422675