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  4. Recombinant Mouse Muscarinic acetylcholine receptor M2 (Chrm2)

Recombinant Mouse Muscarinic acetylcholine receptor M2 (Chrm2)

  • 货号：
    CSB-CF887665MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Chrm2; Chrm-2; Muscarinic acetylcholine receptor M2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-466
  • 氨基酸序列
    MNNSTNSSNNGLAITSPYKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNY FLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFD RYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQF FSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKEKKEPVANQDPVSPSLVQGRI VKPNNNNMPGGDGGLEHNKIQNGKAPRDGGTENCVQGEEKESSNDSTSVSAVASNMRDDE ITQDENTVSTSLGHSKDDNSRQTCIKIVTKTQKGDACTPTSTTVELVGSSGQNGDEKQNI VARKIVKMTKQPAKKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNT VWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
  • 基因功能参考文献：
    1. The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings. PMID: 27339059
    2. In the present study, the functional coupling of Gi/o protein-coupled receptors to GalR1, and the CB1 receptor subtype for endocannabinoids were analyzed in the 3xTg-AD mice model of Alzheimer's disease in the prodromal and advanced stages. In addition, the activity mediated by Gi/o protein-coupled M2/4 muscarinic receptor subtypes was also analyzed in brain areas involved in anxiety and cognition. PMID: 27223629
    3. We found that knockout of the M2/3 receptor significantly inhibited ST37 acupuncture-induced enhancement of gastric motility, jejunal motility, and colonic motility. PMID: 27978539
    4. Muscarinic M2 receptors in the circular muscular layer of mouse ileum exert a previously undocumented function in gut motility via the regulation of gap junctions PMID: 26475617
    5. IL-17 production in the hearts of M2AChR mice is dependent on P2x7 expression in dilated cardiomyopathy. PMID: 26592184
    6. Expression of the M2 receptors mediating cholinergic contractile stimuli of the detrusor muscle was dysregulated in both Mras-/- males and females, although only males exhibited a urinary phenotype. PMID: 26516777
    7. These results demonstrate that M2R are dysfunctional and do not affect ACh levels in PRiMA knockout mice, likely because of down-regulation and/or loss of receptor-effector coupling. PMID: 26506622
    8. Beta-2-Adrenergic and M2-muscarinic receptors decrease basal t-tubular L-type calcium channel activity and suppress ventricular contractility in heart failure. PMID: 24389135
    9. Data indicate taht m2R-RGS6-IKACh pathway sets heart rate variability independently from the autonomic input. PMID: 24204714
    10. the M3 receptor has a pro-inflammatory role in cigarette smoke-induced neutrophilia and cytokine release, yet M1 and M2 receptors have an anti-inflammatory role PMID: 23397297
    11. M2 receptors play an essential role in the generation of intestinal rhythmic motor activity, and M3 receptors have a modulatory role in controlling the periodicity of the rhythmic activity PMID: 23889852
    12. These results suggest for the first time a role of M2 in growth-related changes in extracellular matrix genes during myopia development in a mammalian model. PMID: 23649821
    13. M2-mediated contractions in the M3KO and WT strains were decreased by the Rho kinase (ROCK) inhibitor Y27632 as well as the ROCK, PKA and PKG inhibitor H89. PMID: 23786223
    14. M2 receptor is co-localized with VGLUT2 on intraganglionic laminar endings of esophagus. PMID: 23742744
    15. The m2 receptor is concentrated at cholinergic synapses located on the perikarya and most proximal dendrites, and enriched at glutamatergic synapses both in perikarya and dendrites. PMID: 23184757
    16. Presence of M2 mAChR is sufficient and necessary to mediate the carbachol-induced membrane hyperpolarization and inhibition of SpECTs in laterodorsal tegmental nucleus neurons. PMID: 22956788
    17. In muscararinic M2 receptor knockout mice, although the main cardioinhibitory receptors are not present in the heart, the function is not much affected PMID: 23093370
    18. This study demonstrated that cholinergic projections recruit M(2) receptors to redistribute the gain of LTP in CA3 pyramidal cells in an input-specific manner PMID: 22490561
    19. The function of M2 MR is substituted by beta3-AR and that these effects are mediated via adenylyl cyclase rather than NOS. PMID: 22222438
    20. The results suggested that myocarditis and dilated cardiomyopathy associated with the presence of autoantibodies aginst M2 muscarinic acetylcholine receptors are autoimmune diseases. PMID: 22328321
    21. enhanced tracheal contraction of the BK beta1 knockout can be ascribed to a defect in BK channel opposition of M2 receptor-mediated contractions PMID: 21300746
    22. Caveolae disruption decreased muscarine-induced bronchoconstriction in wild-type and abolished it in M2R(-/-) and M3R(-/-) mice. Thus M2R and M3R signaling pathways require intact caveolae. PMID: 20023174
    23. We therefore conclude that M1 and M4 receptors are the major mAChR subtypes responsible for direct cholinergic modulation of the excitatory hippocampal circuit. PMID: 21160001
    24. Lack of association of a spontaneous mutation of the Chrm2 gene with behavioral and physiologic phenotypic differences in inbred mice. PMID: 20819376
    25. determination of m2 and m3 muscarinic receptors in the central auditory system PMID: 20665816
    26. These findings indicate that cholinergic modulation through M2 receptors regulates feedforward inhibition in a context-dependent manner. PMID: 20386723
    27. Data suggest HL-1 cells express GIRK1/4 and M2 muscarinic receptors and are a good model to study acetylcholine-activated potassium currents. PMID: 20186548
    28. Striatal mAChRs, by inhibiting ACh release from cholinergic interneurons and thus modifying nAChR activity, offer variable control of DA release probability that promotes how DA release reflects activation of dopaminergic axons. PMID: 20203199
    29. During gating, the alpha-helix of the delta subunit M2 segment bends or swivels about its central amino acid residues, with the conformational change of the extracellular half preceding the movement of the intracellular half upon opening. PMID: 11969415
    30. Prefrontal cortex acetylcholine release, EEG slow waves, and spindles are modulated by M2 autoreceptors in C57BL/6J mouse. PMID: 12037184
    31. muscarinic analgesia is exclusively mediated by a combination of M(2) and M(4) mAChRs at both spinal and supraspinal sites. PMID: 12391271
    32. The role of M2 muscarinic receptor in smooth muscle contraction is analyzed in M2 knockout mice. PMID: 12486155
    33. Regional brain localization of FP-TZTP in M2 receptor knockout mice is significantly decreased compared to wild-type in amygdala, brain stem, caudate putamen, cerebellum, cortex, hippocampus, hypothalamus, superior colliculus, and thalamus. PMID: 12668051
    34. mAChRs of the M2 subtype mediate muscarinic agonist-induced bradycardia, tremor, hypothermia, and autoinhibition of release in several brain regions. Review. PMID: 12675128
    35. presynaptic M(2)-muscarinic receptors play a major role in the control of the time course of evoked acetylcholine release under physiological conditions. PMID: 12686574
    36. These data demonstrate a major role for M2 muscarinic receptor subtypes in mediating parasympathomimetic effects. PMID: 12713643
    37. Acute and chronic cholinergic stimulation provoke, in vivo, depletion of the membrane store of somatodendritic m2R through different intracellular mechanisms. PMID: 12794734
    38. M2 receptors play a key role in regulating pharmacologically & physiologically evoked hippocampal acetylcholine release required for the development and expression mnemonic & cognitive events. PMID: 12874603
    39. The muscarinic acetylcholine receptor M2 is a mediator of bradycardia and bronchoconstriction due to vagal innervation. PMID: 14977875
    40. M2 receptors are required for intact working memory, behavioral flexibility, and hippocampal synaptic plasticity. PMID: 15537882
    41. This study indicate that both M2 mediate the muscarinic autoinhibition in ACh release in the longitudinal muscle with myenteric plexus preparation of the mouse ileum. PMID: 15574798
    42. Because ACTH and corticosterone responses were greater in male and female M(2) knockout mice, the M(2) receptor appears to dampen the stress response PMID: 16280029
    43. Muscarinic M2 receptor (M2R)-mediated transactivation of EGFR specifically involves Fyn but not c-Src or Yes. PMID: 16337776
    44. Hearts of chronic chagasic mice had increased muscarinic receptor densities compared to normal controls. PMID: 16978452
    45. Substance P-mediated sustained contraction is negatively regulated by the M2 receptor at a presynaptic level in the ileum. PMID: 17008557
    46. This study provides new information that the M(2) mAChRs play a critical role in the control of glutamatergic input from primary afferents to dorsal horn neurons. PMID: 17050831
    47. affects cognition, as seen in knockout mice PMID: 17192665
    48. M2R was observed exclusively in the umbrella cells. PMID: 17337281
    49. Intestinal myocytes have 3 distinct muscarinic pathways mediating cationic channel activation. M2/M3 pathway targeting 70-pS channels serves as the major contributor to membrane potentials. PMID: 17463038
    50. M(2)-AChR may mediate an inhibitory regulation on matrix metalloproteinase function PMID: 18055517

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM2 sub-subfamily
  • 数据库链接：

    KEGG: mmu:243764

    STRING: 10090.ENSMUSP00000130874

    UniGene: Mm.448632