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  4. Recombinant Mouse Muscarinic acetylcholine receptor M1 (Chrm1)

Recombinant Mouse Muscarinic acetylcholine receptor M1 (Chrm1)

  • 货号：
    CSB-CF005381MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Chrm1; Chrm-1; Muscarinic acetylcholine receptor M1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-460
  • 氨基酸序列
    MNTSVPPAVSPNITVLAPGKGPWQVAFIGSTTGLLSLATVTGNLLVLISIKVNTELKTVN NYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLIS FDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYI QFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETENRARELAALQGSETPGKGGGSSSS SERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEV VIKMPMVDPEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKE KKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTVNPMCYAS CNKAFRDHFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
  • 基因功能参考文献：
    1. Results indicate that direct inhibition of acetylcholinesterase activity and up-regulation of m1 muscarinic acetylcholine receptor expression in the striatum might contribute to the beneficial effects of alpha-asarone on locomotor hyperactivity in Fmr1 knock out mice. PMID: 27316341
    2. The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings. PMID: 27339059
    3. The memory deficits in mouse prion disease were completely restored by treatment with benzyl quinolone carboxylic acid (BQCA) and benzoquinazoline-12 (BQZ-12), two highly selective positive allosteric modulators (PAMs) of M1 mAChRs. Furthermore, prolonged exposure to BQCA markedly extended the lifespan of diseased mice. PMID: 27991860
    4. Data suggest that the inositol phosphate accumulation assay has utility for the evaluation of M1 muscarinic acetylcholine receptor activators in vivo. PMID: 27958713
    5. determining the phosphorylation status of the M1 mAChR at Ser(228) not only provides a means of establishing receptor activation following drug treatment both in vitro and in vivo but also allows for the mapping of the activation status of the M1 mAChR in the hippocampus PMID: 26826123
    6. This study showed that M1 receptor knockout mice have higher noradrenaline levels than that of the wildtype mice, in parallel to a decrease in IgG producing B cells. PMID: 26002586
    7. M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice PMID: 26374068
    8. High M1 muscarinic receptor expression is associated with acute liver injury. PMID: 25452146
    9. This study demonistrated that Expression of m1-type muscarinic acetylcholine receptors by parvalbumin-immunoreactive neurons in the primary visual cortex PMID: 23983014
    10. The mm (chi(1) congruent with -60 degrees ; chi(2) congruent with -60 degrees ) and tp (chi(1) congruent with 180 degrees ; chi(2) congruent with +60 degrees ) rotamers were identified as the likely conduction-catalyzing conformations of the AChR's selectivity-filter glutamates. PMID: 25049389
    11. the M3 receptor has a pro-inflammatory role in cigarette smoke-induced neutrophilia and cytokine release, yet M1 and M2 receptors have an anti-inflammatory role PMID: 23397297
    12. The results of this study suggested that M1-muscarinic receptors promote fear memory consolidation via phospholipase C and the M-current. PMID: 24478341
    13. mAChRs in mouse colonic epithelial cells consist of two subtypes, M1 (80 %) and M3 (20 %); The M1 subtype was likely to regulate epithelial chloride secretion negatively and was susceptible to inflammation PMID: 23242454
    14. M1 receptor is co-localized with VGLUT2 on the intraganglionic laminar endings of esophagus. PMID: 23742744
    15. Downregulation of M1 muscarinic receptor in klotho mutant mice and inactivation of the JAK2/STAT3 signaling axis plays a critical role in cognitive impairment. PMID: 23389690
    16. In the prefrontal cortex, M1 receptor enhances cyclic AMP formation and signaling to the nucleus. PMID: 22456324
    17. Structural rearrangement analysis by FRET revealed that Gq coupling stabilizes the active conformation of muscarinic M1 receptor. PMID: 23085334
    18. M1-mAChRs function at both surface and intracellular sites in telencephalon neurons including the hippocampus PMID: 23678982
    19. a low concentration of N-methyl-d-aspartate causes long-lasting hippocampal cell damage and that endogenous acetylcholine plays, via muscarinic M(1) receptor, a rescuing role in the excitotoxicity-induced long-lasting hippocampal cell damage PMID: 23220711
    20. These data suggest that M1R(-/-) mice display abnormal responding in the face of relatively preserved attention, learning and perception. PMID: 21903112
    21. Data show that CFP/YFP-M(1) mAChR chimera expressed predominantly at the plasma membrane . PMID: 22272263
    22. disrupting the M(1)R plays a role in exacerbating Alzheimer's disease-related cognitive decline and pathological features PMID: 21704011
    23. the P2X7R-Panx1 complex may play an important role as a negative modulator of M1 receptor-mediated seizure activity in vivo PMID: 21505260
    24. We therefore conclude that M1 and M4 receptors are the major mAChR subtypes responsible for direct cholinergic modulation of the excitatory hippocampal circuit. PMID: 21160001
    25. The gating rate and equilibrium constants for receptors with point mutations of alpha-subunit residues located between the binding sites and the membrane domain. PMID: 20682257
    26. The results of this study indicated that the M1 mAChR is an important regulator of amyloidogenesis in the brain and provide strong support for targeting the M1 mAChR as a therapeutic candidate in Alzheimer disease. PMID: 20335454
    27. Muscarinic M1 receptor is so positioned in the cortex as to sense ambient ACh released from cholinergic terminals at variable distances and to enhance the synaptic efficacy and excitability of pyramidal cells. PMID: 20335477
    28. Our data provide important insight into the molecular basis of gamma oscillations by unequivocally establishing a novel role for muscarinic modulation of I(h) and I(cat) in rhythmic network activity. PMID: 11856534
    29. activation on sympathetic postganglionic cells results in catecholamine-mediated cardiac stimulation PMID: 11907166
    30. Just 15% of the M1 receptors in mouse hippocampus was required for maximal binding of oxotremorine-M-stimulated GTP-gamma-35S, suggesting that the M1 receptor is the primary G(alpha)(q)/11-coupled muscarinic receptor in mouse cerebrum. PMID: 12106668
    31. Muscarinic receptors are the predominant receptor mediating contractile responses in female mouse urinary bladder smooth muscle, with strain differences. PMID: 12359634
    32. The presence of muscarinic M1 receptor-mediated relaxation in the stomach fundus supports the neuronal localization of a muscarinic M1 receptor that activates nitric oxide release to effect relaxation. PMID: 12538821
    33. The M1 mAChRs modulate neurotransmitter signaling in cortex and hippocampus. Review. PMID: 12675128
    34. Muscarinic M1 receptors activate phosphoinositide hydrolysis in cortex and hippocampus of mice, consistent with the role of M1 receptors in cognition. Muscarinic M1 receptors appear to be the only muscarinic receptor subtype mediating seizures. PMID: 12713643
    35. RGS2 binds directly to the third intracellular (i3) loop of the G(q/11)-coupled M1 muscarinic cholinergic receptor (M1 mAChR; M1i3). PMID: 14976183
    36. M1 muscarinic receptors selectively modulate CaV1.3 in striatal medium spiny neurons. PMID: 15689540
    37. In gastric chief cells a mixture of M1 and M3 receptors mediates cholinergic stimulation of pepsinogen secretion and no other muscarinic receptor subtypes are involved in this activity PMID: 15933222
    38. KCNQ channel currents in striatal medium spiny neurons were potently reduced by M1 muscarinic receptors. PMID: 16093396
    39. agrin and perlecan cluster with laminin-2 to contribute to assembly of aneural AChR clusters that precede neural agrin release as well as affect later NMJ development PMID: 16219760
    40. Physiologically released acetylcholine from cholinergic fibers modulates hippocampal synaptic plasticity through the postsynaptic M1 mAChR activation. PMID: 16319319
    41. The afterdepolarization in stratum oriens-lacunosum moleculare cells, mediated by M1/M3 receptors, was associated with inhibition of an M current, a slow calcium-activated potassium current, and a calcium-dependent non-selective cationic current PMID: 16322052
    42. Data show that cocaine-induced status epilepticus and lethality produces differential changes in brain muscarinic M(1)and dopaminergic D1 and 2 receptors, depending on the brain area studied. PMID: 16633898
    43. can trigger glandular secretions. PMID: 17192665
    44. Results suggest that M1 and M5 muscarinic cholinergic receptors are not involved in anti-viral immunity. PMID: 17286988
    45. The decreased pituitary-adrenal sensitivity to oxotremorine and restraint stress noted in M(1) knockout mice is consistent with M(1) being primarily a postsynaptic receptor. PMID: 18363805
    46. the decrease in ACh-induced relaxation following perinatal hypoxia, suggesting that M(1)AChR-mediated alteration of ACh-induced relaxation is due to the activation of calcium-dependent PDE1. PMID: 18469116
    47. It is concluded that M1 muscarinic receptors may play a time-dependent role in the consolidation of reward-related memory of morphine. PMID: 18502314
    48. results demonstrate that M1 receptors facilitate cue detection behaviors and are both necessary and sufficient for most direct effects of ACh on pyramidal neuron excitability PMID: 19657040
    49. dysfunction of mAChRs might be a substantial basis for the progressive neurological deterioration in mice with double knockout of GD2 and GD3 synthase. PMID: 19744524

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM1 sub-subfamily
  • 数据库链接：

    KEGG: mmu:12669

    STRING: 10090.ENSMUSP00000042632

    UniGene: Mm.240607