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  4. Recombinant Mouse Linker for activation of T-cells family member 1 (Lat)

Recombinant Mouse Linker for activation of T-cells family member 1 (Lat)

  • 货号：
    CSB-CF012767MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Lat; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-242
  • 氨基酸序列
    MEADALSPVGLGLLLLPFLVTLLAALCVRCRELPVSYDSTSTESLYPRSILIKPPQITVPRTPAVSYPLVTSFPPLRQPDLLPIPRSPQPLGGSHRMPSSQQNSDDANSVASYENQEPACKNVDADEDEDDYPNGYLVVLPDSSPAAVPVVSSAPVPSNPDLGDSAFSVESCEDYVNVPESEESAEASLDGSREYVNVSPEQQPVTRAELASVNSQEVEDEGEEEGVDGEEAPDYENLQELN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
  • 基因功能参考文献：
    1. LatY136F knock-in mice displayed increased production of Th2-type IgG1 (a homologue of human IgG4) and developed multiple organ tissue lesions reminiscent of those seen in patients with of immunoglobulin G4-related disease (IgG4-RD). The development of these tissue lesions was highly sensitive to corticosteroid treatment like in IgG4-RD. LatY136F knock-in mouse strain represents a promising model for human IgG4-RD. PMID: 29902238
    2. this study shows that the site-specific delivery of linker for activation of T cells (LAT) in asthmatic mouse model PMID: 28063403
    3. IFT20 is required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes. PMID: 26715756
    4. provide evidence that CD28 and the TCR complex regulate NF-kappaB via different signaling modules of GRB-2/VAV1 and LAT/ADAP pathways respectively. PMID: 25455592
    5. low level of LAT-PLC-gamma1 interaction was associated with Th2 polarized differentiation, and this may contribute to the etiology of asthma. PMID: 24978613
    6. These results suggest that proteasome-mediated degradation is involved in hypophosphorylated LAT and PLCgamma1 in Dow2-induced anergic T cells. The novel CD3-specific Ab, Dow2, may provide us with a unique tool for inducing immunosuppression PMID: 24595757
    7. Sos1 has distinct roles in acting as a scaffold to oligomerize the adaptor protein LAT, and in guanine nucleotide exchange activity PMID: 24222714
    8. this analysis identified 65 proteins not associated before with the Zap70-Lat-SLP-76 network and thus should provide cues for future functional experiments. PMID: 24584089
    9. Data suggest that transmembrane adaptor protein LAT-PLCgamma1 (Phospholipase C gamma 1) signaling may function differently in various subsets of gammadelta T cells. PMID: 24523509
    10. chemotaxis toward antigen is controlled in mast cells by a cross-talk among FcepsilonRI, tetraspanin CD9, transmembrane adaptor proteins NTAL and LAT, and cytoskeleton-regulatory proteins of the ERM family PMID: 23443658
    11. LAT-mediated signaling is essential for the continuous expansion of CD8 T cells during T cell priming, but is not required for contraction and memory maintenance of CD8 T cells. PMID: 23401587
    12. The lymphoproliferative disease observed in LAT-Y136F mutant mice is at least partially dependent on hyperactivation of Ras. PMID: 23209318
    13. our data suggest that LAT acts as a positive regulator of RANKL-induced osteoclastogenesis. PMID: 22382685
    14. LAT-deficient CTLs failed to upregulate FasL and produce gamma interferon after engagement with target cells and had impaired granule-mediated killing. PMID: 22566687
    15. PECAM-1-mediated inhibition of GPVI-dependent platelet responses result from recruitment of SHP-2-p85 complexes to tyrosine-phosphorylated PECAM-1, which diminishes the association of PI3K with activatory signaling molecules Gab1 and LAT PMID: 20723025
    16. Data implicate that LAT has positive and negative roles in the regulation of mature T cells. PMID: 20837489
    17. While SLP-76 and LAT1 depend on each other for many of their functions, LAT2/SLP-76 interactions and SLP-76-independent LAT1 functions also mediate a positive signaling pathway downstream of FcepsilonRI in mast cells. PMID: 20606011
    18. mouse models revealed that LAT constitutes more than just a positive regulator of TCR signaling and plays a negative regulatory role that contributes to terminate antigen-driven T cell responses [Review] PMID: 20107804
    19. Data indicate that the binding of LAT to PLC-gamma1 is essential for the suppressive function of CD4(+)CD25(+) regulatory T cells. PMID: 20130215
    20. Data show that In quiescent T cells, both TCR and Lat existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. PMID: 20010844
    21. LAT is required for signaling via the T cell antigen receptor (TCR) PMID: 11901197
    22. inhibitory function for LAT that is critical for the differentiation and homeostasis of TH cells PMID: 12065839
    23. critical role for integrated PLC-gamma1 and Ras-Erk signaling through LAT in T cell development and homeostasis PMID: 12065840
    24. LAT colocalizes with 2B4 in glycolipid-enriched microdomains of the plasma membrane, is a required intermediate for 2B4 signal transduction, and plays a role in 2B4-mediated cytotoxicity. PMID: 12077228
    25. adaptor proteins LAT and SLP-76 are involved in pre-BCR signaling, thereby rescuing arrested murine SLP-65(-/-) pre-B cells PMID: 12932362
    26. The four distal tyrosines of this protein are required for signaling in FcepsilonRI-mediated mast cell activation. PMID: 12953098
    27. LAT is an essential regulator of T cell homeostasis and terminal differentiation. PMID: 12970761
    28. Vav1 transduces TCR signals to Ras by controlling recruitment of RasGRP1 to the membrane and recruitment of Sos1 and -2 to the transmembrane adapter protein LAT. PMID: 14764585
    29. LAT controls its own recruitment at the immune synapse, where it is required as a scaffold protein for the signalling machinery. PMID: 14996932
    30. LAT tyrosines differentially contribute to exocytosis and cytokine secretion and differentially regulate biological responses of mucosal- and serosal-type mast cells. PMID: 15470052
    31. The Lat does not completely ablate mast cells responsiveness. PMID: 15745852
    32. Mutation of the phospholipase C-gamma1-binding site of LAT affects both positive and negative thymocyte selection. PMID: 15795236
    33. Role of LAT in the development and function T cells. Review. PMID: 16102570
    34. mouse LAT mutant lacking palmitoylation was unstable and susceptible to degradation via the proteasome pathway. PMID: 16460687
    35. Polyclonal B cell activation in Lat(Y136F) mutant mice is not due to a direct (intrinsic) effect of the Lat mutation on B cells but indirectly results from the presence of CD4 Th2 effector cells. PMID: 16887989
    36. LAT mediates immunoglobulin E-induced sustained Erk activation critical for mast cell survival. PMID: 17420272
    37. activation of phospholipase C gamma 2 via glycoprotein 6 is dependent on 2 complementary events PMID: 17579183
    38. Despite the presence of autoantibodies causative of severe systemic disease, the pathological conditions observed in Lat(Y136F) mice unfold in an Ag-independent manner and thus do not qualify as a genuine autoimmune disorder. PMID: 18209052
    39. Overexpression of either Sp1 or full-length Sp3 was shown to augment LAT promoter activity, while siRNA-mediated knockdown of each transcription factor was demonstrated to have an inhibitory effect. PMID: 18343609
    40. GPVI-FcR gamma-chain, LAT and PLCgamma2 are essential for thrombus generation and stability PMID: 18464161
    41. These data show that natural killer cell immunoreceptor tyrosine-based activation motifs preferentially use a signaling cassette regulated by interplay between LAT and LAB. PMID: 18645037
    42. Gads plays a key role in linking the adapter LAT to SLP-76 in response to weak activation of GPVI and CLEC-2 whereas LAT is required for full activation over a wider range of agonist concentrations. PMID: 18826392
    43. Deletion of the STAT6 transcription factor converts the fulminant T helper(Th)2 cell lymphoproliferative disorder that characterizes LatY136F mice into a fast-onset lymphoproliferative disorder dominated by competent Th1 effectors and CD8 T cells. PMID: 19234162
    44. Supplementation with vitamin E eliminates the age-related differences in LAT phosphorylation in T cells from young and old. PMID: 19403707
    45. Results identify novel functions for both CD8 and LAT during CD8-mediated apoptosis that are independent of TCR signal transduction and suggest a mechanism for signal transduction leading to apoptosis upon CD8 crosslinking. PMID: 19449311
    46. LAT palmitoylation functions primarily as a sorting signal required for its plasma membrane transport PMID: 19592663
    47. Data show that Erk activation was diminished in LAT knock-in Bam32 knockout CD4(+) T cells. PMID: 19667175
    48. The fact that such LAT-independent signals result in lymphoproliferative disorders with excessive cytokine production demonstrates that LAT constitutes a key negative regulator of the triggering module. PMID: 19682930
    49. Results demonstrate a combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a activation to generate inflammation. PMID: 19795417

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  • 亚细胞定位：
    Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
  • 组织特异性：
    Expressed in T-cells and mast cells.
  • 数据库链接：

    KEGG: mmu:16797

    STRING: 10090.ENSMUSP00000032997

    UniGene: Mm.10280