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  4. Recombinant Mouse Hydroxycarboxylic acid receptor 2 (Hcar2)

Recombinant Mouse Hydroxycarboxylic acid receptor 2 (Hcar2)

  • 货号：
    CSB-CF866800MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Hcar2; Gpr109; Gpr109a; Gpr109b; Niacr1; Pumag; Hydroxycarboxylic acid receptor 2; G-protein coupled receptor 109; G-protein coupled receptor 109A; G-protein coupled receptor HM74; Niacin receptor 1; Nicotinic acid receptor; Protein PUMA-G
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-360
  • 氨基酸序列
    MSKSDHFLVINGKNCCVFRDENIAKVLPPVLGLEFVFGLLGNGLALWIFCFHLKSWKSSR IFLFNLAVADFLLIICLPFLTDNYVHNWDWRFGGIPCRVMLFMLAMNRQGSIIFLTVVAV DRYFRVVHPHHFLNKISNRTAAIISCFLWGLTIGLTVHLLYTNMMTKNGEAYLCSSFSIC YNFRWHDAMFLLEFFLPLAIILFCSGRIIWSLRQRQMDRHAKIKRAINFIMVVAIVFIIC FLPSVAVRIRIFWLLYKYNVRNCDIYSSVDLAFFTTLSFTYMNSMLDPVVYYFSSPSFPN FFSTCINRCLRKKTLGEPDNNRSTSVELTGDPSTTRSIPGALMADPSEPGSPPYLASTSR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis.
  • 基因功能参考文献：
    1. HCA2 plays a critical role in host protection against pro-inflammatory insults. PMID: 28186140
    2. These results demonstrate that GPR109A is functionally expressed in both human and murine islet beta-cells. PMID: 27570060
    3. This study showed that the hypophagia of heat stress is independent of GPR109a, the hepatic vagus afferent nerve, and hepatic ketone body synthesis. PMID: 26936786
    4. Enhanced GPR109a expression in jejunal enterocytes of T2DM mice suggests that GPR109a is involved in elevating intestinal glucose transport observed in diabetes. PMID: 26371038
    5. The expression of GPR109A in pancreatic beta cells is not only influenced by inflammation and glucose, but also plays a protective role under inflammatory conditions. PMID: 26116596
    6. These results suggest that the PKC pathway and PDGFR/EGFR transactivation pathway play important roles in HCA2-mediated Akt activation. PMID: 25375133
    7. Data indicate that GPR109a modulates niacin-induced pancreatic islet dysfunction through activation of the islet beta-cells. PMID: 25622782
    8. Data suggest that expression of Gpr109a is upregulated in adipocytes (intra-abdominal adipose tissue) and macrophages (peritoneal macrophages) during inflammation (models of gram-positive, gram-negative, viral, and fungal infections). PMID: 25320346
    9. HCAR2 activation by dietary or pharmacological means instructs Ly-6C(Lo) monocytes and/or macrophages to deliver a neuroprotective signal to the brain. PMID: 24845831
    10. data indicate that HCA mediates the therapeutic effects of DMF in EAE. PMID: 24691444
    11. GPR109A is a tumor suppressor in mammary gland. PMID: 24371223
    12. role in mediating the beneficial effects of gut microbiota and dietary fiber in colon PMID: 24412617
    13. chronic obesity reduces GPR109A and GPR81 expression in the adipose tissue, while acute in vitro LPS treatment increases expression of GPR109A in adipocytes and macrophages. PMID: 22842580
    14. 0.5-1% niacin in the daily diet activates the mouse GPR109A receptor. PMID: 21774590
    15. Data show that high levels of PUMA-G transcripts and protein were detected in all beta cells. PMID: 21441844
    16. Nicotinic acid inhibits progression of atherosclerosis in mice through its receptor GPR109A expressed by immune cells PMID: 21317532
    17. In conventional mice with normal bacterial colonization of the intestinal tract, GPR109A and SLC5A8 are expressed on the apical membrane of epithelial cells lining the ileum and colon. PMID: 20033346
    18. PUMA-G is highly expressed in adipose tissue and is a nicotinic acid receptor PMID: 12563315
    19. (D)-beta-hydroxybutyrate ((D)-beta-OHB) specifically activates PUMA-G/HM74a at concentrations observed in serum during fasting; (D)-beta-OHB inhibits mouse adipocyte lipolysis in a PUMA-G-dependent manner PMID: 15929991
    20. GPR109A mediates nicotinic acid-induced flushing, an effect which involves release of PGE(2) and PGD(2), most likely from immune cells of the skin PMID: 16322797
    21. Data show that many phenolic acids, including those from the hydroxybenzoic and hydroxycinnamic acid classes, can bind and activate GPR109A (HM74a/PUMA-G), the receptor for the antidyslipidemic agent nicotinic acid. PMID: 19136666
    22. GPR109A receptor plays an important role in the dual regulation of adiponectin secretion and lipolysis PMID: 19141678
    23. studies demonstrated that GPR109A is expressed in retina with its expression restricted to retinal pigment epithelium, where it differentially polarizes to the basolateral membrane PMID: 19223991
    24. the ligand receptor pair 3-OH-octanoic acid/GPR109B mediates a negative feedback regulation of adipocyte lipolysis in human but not in mouse PMID: 19561068

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed in lungs, spleen, heart, skeletal muscle and adipose tissue.
  • 数据库链接：

    KEGG: mmu:80885

    STRING: 10090.ENSMUSP00000054104

    UniGene: Mm.440567