Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 跨膜蛋白
  4. Recombinant Mouse Hereditary hemochromatosis protein homolog (Hfe)

Recombinant Mouse Hereditary hemochromatosis protein homolog (Hfe)

  • 货号：
    CSB-CF010323MO
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Hfe
  • Uniprot No.：
  • 别名：
    Hfe; Mr2; Hereditary hemochromatosis protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    39.5 kDa
  • 表达区域：
    25-359aa
  • 氨基酸序列
    QALPPRSHSLRYLFMGASEPDLGLPLFEARGYVDDQLFVSYNHESRRAEPRAPWILEQTSSQLWLHLSQSLKGWDYMFIVDFWTIMGNYNHSKVTKLGVVSESHILQVVLGCEVHEDNSTSGFWRYGYDGQDHLEFCPKTLNWSAAEPGAWATKVEWDEHKIRAKQNRDYLEKDCPEQLKRLLELGRGVLGQQVPTLVKVTRHWASTGTSLRCQALDFFPQNITMRWLKDNQPLDAKDVNPEKVLPNGDETYQGWLTLAVAPGDETRFTCQVEHPGLDQPLTASWEPLQSQAMIIGIISGVTVCAIFLVGILFLILRKRKASGGTMGGYVLTDCE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    18-23 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
  • 基因功能参考文献：
    1. H67D mutation in HFE gene is associated with decreased susceptibility to manganese accumulation in the brain and neurotoxicity induced by inhaled manganese. PMID: 27295312
    2. the aging HFE KO mouse on an SV129 genetic background has the potential to facilitate the investigation of cardiomyopathy induced by HFE gene mutations. PMID: 28558946
    3. unlike homozygous Hfe deletion, heterozygous gene deletion disrupted glucose homeostasis but did not affect lipid metabolism or liver injury. PMID: 27354540
    4. Single Hjv(-)/(-) and double Hfe(-)/(-)Hjv(-)/(-) mice exhibit comparable iron overload. Hfe and Hjv regulate hepcidin via the same pathway. PMID: 25609138
    5. Results show that HFE requires HJV to activate downstream signal transduction pathways for hepcidin regulation. PMID: 25608116
    6. Alterations in cholesterol metabolism associated with expression of H63D-HFE may contribute to the development of AD. PMID: 24439478
    7. These results support in vivo studies which suggest that Hfe and Tfr2 can independently regulate hepcidin. PMID: 24155934
    8. A mutation in the HFE gene is associated with altered brain iron profiles and increased oxidative stress in mice. PMID: 23429074
    9. Hfe-knockout mice did not have higher brain iron levels than wildtype controls. PMID: 22466002
    10. Hfe(-/-) retinal pigment epithelial cells exhibited slower senescence rate and higher survivin expression than wild type cells. Hfe(-/-) cells migrated faster and showed greater glucose uptake and increased expression of GLUTs. PMID: 23169885
    11. Findings suggest a novel role for Hfe and/or imbalanced iron homeostasis in the regulation of the inflammatory response in the lung and hereditary hemochromatosis. PMID: 22745741
    12. Double mutant mice lacking functional Hfe or Tfr2 and Tmprss6 exhibited a severe iron deficiency microcytic anemia phenotype mimicking the phenotype of single mutant mice lacking functional Tmprss6 demonstrating that Hfe and Tfr2 are not substrates for Tmprss6. PMID: 22244935
    13. Disruption of both Hfe and Tfr2 caused more severe hepatic iron overload with more advanced lipid peroxidation, inflammation, and portal fibrosis than was observed with the disruption of either gene alone. PMID: 22383097
    14. the Hfe(-/-) mouse brain showed numerous significant changes in transcript levels although few of these related to proteins directly involved in iron homeostasis PMID: 22370144
    15. Loss of central and peripheral CD8+ T-cell tolerance to HFE in mouse models of human familial hemochromatosis. PMID: 22531912
    16. Hfe(-/-) mice show defective hepatic-intestinal iron and lipid signaling, which predispose them toward diet-induced hepatic lipotoxicity, accompanied by an accelerated progression of injury to fibrosis. PMID: 21817060
    17. the HFE H63D mutant protein is associated with prolonged ER stress and chronically increased neuronal vulnerability. PMID: 21349849
    18. neither genetic loss of Hfe nor hepatic Hfe overexpression modulated the hepcidin elevation and systemic iron deficiency of Tmprss6(-/-) mice PMID: 21355094
    19. Hfe influences erythropoiesis by 2 distinct mechanisms: limiting hepcidin expression under conditions of simultaneous iron overload and stress erythropoiesis, and impairing transferrin-bound iron uptake by erythroid cells. PMID: 21059897
    20. transferrin receptor 2 and HFE are involved in holotransferrin-dependent signaling for the regulation of furin which involved Erk phosphorylation. Furin in turn may control hepcidin expression. PMID: 20634490
    21. Hepcidin expression does not rescue the iron-poor phenotype of Kupffer cells in Hfe-null mice after liver transplantation. PMID: 20338170
    22. Hfe is limiting in formation of the Hfe/Tfr2 complex that regulates hepcidin expression PMID: 20177050
    23. Multiple pregnancies do not reduce body iron stores in Hfe-/- mice. PMID: 20110460
    24. In Hfe knockout mice Hamp1 mPNA was decreased and duodenal ferroportin mRNA expression was increased compared to wild-type animals. PMID: 19426170
    25. Prolyl-peptidyl isomerase, Pin1, phosphorylation is compromised in association with the expression of the HFE polymorphic allele, H63D. PMID: 20060900
    26. Hfe deficiency causes a previously unrecognized increase in gene expression of hepatic acute phase proteins and duodenal digestive enzymes PMID: 19787063
    27. Iron uptake from plasma transferrin by the duodenum is impaired in the Hfe knockout mouse. PMID: 11943867
    28. Mouse hemachromatosis protein (Hfe) plays a minor role in down-regulation but does not influence the up-regulation of iron absorption PMID: 12149232
    29. evidence that HFE regulates a functional cross-talk between crypt and villi enterocytes PMID: 12367579
    30. In normal & Hfe knock-out mice, duodenal nonheme iron content correlated with liver iron stores. This did not depend on dietary iron levels. However, duodenal iron content was reduced in Hfe knock-out mice for any given content of liver iron stores. PMID: 12468424
    31. Mice lacking Hfe or producing a C282Y mutant Hfe protein develop hyperferremia and have high hepatic iron levels. PMID: 12704388
    32. Oxidative damage in colon and mammary tissue in null-mutant mice PMID: 12706501
    33. Results show no correlation between the expression levels of the duodenal iron transporters DMT1 and IREG1 and the liver iron content in Hfe (hemochromatosis) knockout mice. PMID: 14618243
    34. In mice, deficiency of Hfe is associated with the same pattern of iron overload observed in patients with hereditary hemochromatosis. PMID: 14656876
    35. Rag1 deficiency in Hfe knock-out mice leads to heightened iron overload, mainly in the liver. PMID: 14656877
    36. H67D mutation leads to partial loss of Hfe function and can contribute to murine hereditary hemochromatosis. PMID: 14673107
    37. Hfe deficient mice react normally to endotoxin and their hepatocytes react normally to IL-6. PMID: 15192150
    38. Treatment of C57BL/6 Hfe(-/-) mice with 15% ethanol for 6.5 months did not increase hepatic uroporphyrin PMID: 15382179
    39. over-expression of mHFE, but not of hHFE, in a mouse transformed cell line, results in a most significant inhibition of transferrin-uptake which correlated with apoptotic cell death PMID: 15389541
    40. These results are the first in a series of studies to understand how mutations in Hfe can be a risk factor for AD. PMID: 15718038
    41. Reconstitution of Hfe-deficient mice with wt bone marrow resulted in augmented capacity of the spleen to store iron and in significantly decreased liver iron loading, accompanied by a significant increase of hepatic hepcidin mRNA levels. PMID: 15914561
    42. direct recognition of mouse Hfe molecules by cytotoxic T lymphocytes (CTLs) was demonstrated in DBA/2 Hfe knockout mice PMID: 16123136
    43. In conclusion, these results suggest that although Hfe is necessary for the establishment of hepcidin basal levels. PMID: 16565419
    44. HFE and TFR2 interact in cells; this interaction is not abrogated by disease-associated mutations of HFE and TFR2; and that TFR2 competes with TFR1 for binding to HFE PMID: 16893896
    45. The specific localization of HFE and its interacting proteins, TfR1 and TfR2, at the basolateral membrane of RPE is relevant to the regulation of iron homeostasis in this cell. PMID: 17003411
    46. An inverse correlation between transferrin saturation and transferrin half-life for both HFE-deficient and wild-type mice, which suggests that HFE does not have a direct effect on transferrin catabolism PMID: 17116709
    47. in an alcohol-susceptible strain, mutation of the Hfe gene diminished the response of the measured iron indices to alcohol treatment PMID: 17207112
    48. Rgmc regulation by LPS is Hfe-independent. PMID: 17255318
    49. Hfe is required for appropriate expression of the "iron hormone" hepcidin which then controls intestinal iron absorption. PMID: 17264297
    50. Hfe(-/-) mice demonstrate increased adiponectin levels and activation of AMP-dependent kinase PMID: 17971451

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    MHC class I family
  • 数据库链接：

    KEGG: mmu:15216

    STRING: 10090.ENSMUSP00000089298

    UniGene: Mm.2681