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  4. Recombinant Mouse G protein-activated inward rectifier potassium channel 1 (Kcnj3)

Recombinant Mouse G protein-activated inward rectifier potassium channel 1 (Kcnj3)

  • 货号：
    CSB-CF012056MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Kcnj3; Girk1; G protein-activated inward rectifier potassium channel 1; GIRK-1; Inward rectifier K(+ channel Kir3.1; Potassium channel, inwardly rectifying subfamily J member 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-501
  • 氨基酸序列
    MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQGPQQQLVPKKKRQRFVDKNGRCNVQHGNL GSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNY TPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCM FIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPE GEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEVVVILE GIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKE QEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDISTKLPSKLQKITGREDFPKKLLRMS STTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGG PTRMEGNLPAKLRKMNSDRFT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.
  • 基因功能参考文献：
    1. Ethanol activates GIRK1 by shifting the conformational equilibrium processes, which are responsible for the gating of GIRK, to stabilize an open conformation of the cytoplasmic ion gate. PMID: 29581303
    2. Kir 3.1 channels are important for supraspinal antinociception and presynaptic GABA release inhibition by oxycodone in the femur bone cancer model PMID: 25521524
    3. GABA neurons in the ventral tegmental area express GIRK1 (and GIRK2) subunits. PMID: 25948263
    4. K(IR)3.1 channels play a primary role in the antinociceptive effects of oxycodone, but not those of morphine, at supraspinal sites and suggest that supraspinal K(IR)3.1 channels are responsible for the unique analgesic profile of oxycodone. PMID: 24117458
    5. siRNA knock-down of NgR1 resulted in a selective increase of GABAB R1 and GABAB R2 protein and an increase in GIRK1. PMID: 23829864
    6. Discontinuous and sometime opposing elements in Girk1 underlie the Girk1-dependent potentiation of receptor-dependent and receptor-independent heteromeric channel activity. PMID: 23236146
    7. Mechanism for functional dysregulation in the dorsal raphe follows tyrosine phosphorylation of repeated stress-activated Kir3.1 channels. PMID: 22956823
    8. NMR analyses of the Gbetagamma binding and conformational rearrangements of the cytoplasmic pore of G protein-activated inwardly rectifying potassium channel 1 (GIRK1). PMID: 21075842
    9. Agonist-induced localization of Gq-coupled receptors and G protein-gated inwardly rectifying K+ (GIRK) channels to caveolae determines receptor specificity of phosphatidylinositol 4,5-bisphosphate signaling PMID: 21041306
    10. The atrial potassium channel, I(KACH), ion channel gating is accelerated in atrial myocytes through the RGS6/Gbeta5 complex. PMID: 20884879
    11. Dopamine neurons from Girk1 knock-out mice (and Girk2) exhibited elevated glutamatergic neurotransmission and increased synaptic levels of AMPA glutamate receptors. PMID: 20557431
    12. Data suggest HL-1 cells express GIRK1/4 and M2 muscarinic receptors and are a good model to study acetylcholine-activated potassium currents. PMID: 20186548
    13. confers properties to I(KACh) that enhance channel activity and that Kir3.4 homomultimers do not contribute significantly to the muscarinic-gated potassium current PMID: 12374786
    14. Kcnj3 is associated with idiopathic generalised epilepsy syndromes by genotype. PMID: 12458027
    15. A cytoplasmic pore in the GIRK1 channel extends the ion pathway to 60 A, nearly twice the length of a canonical transmembrane K(+) channel. The cytoplasmic pore is lined by acidic and hydrophobic amino acids; polyamines block this pore. PMID: 12507423
    16. We found GIRK1 and GIRK2 subunits in the spinal cord dorsal horn; GIRK1 and GIRK2 knock-out mice exhibited hyperalgesia. Furthermore GIRK1 and GIRK2 knock-out mice showed decreased analgesic responses after spinal administration of higher morphine doses PMID: 15028774
    17. Data show that the composition of the Kir3.1 and Kir 3.4 subunits of the G protein-gated potassium channel changes during embryonic development. PMID: 15467839
    18. phosphorylation of K(ir)3.1 in spinal cord is induced by acute inflammation, chronic neuropathic pain, and behavioral stress PMID: 16223722
    19. GIRK1 exhibited the most widespread and robust labeling in the cerebellum, with labeling particularly prominent in granule cells,Purkinje neurons,basket cells. PMID: 18088366
    20. GIRK channels make important contribution to a range of behaviors and may represent points of therapeutic intervention in disorders of anxiety, spasticity and reward PMID: 18194467
    21. This study investigates the action of CRF on G-protein-linked inhibitory postsynaptic currents (IPSCs) mediated by GIRK (Kir3) channels in dopamine neurons. PMID: 19279570
    22. The association of GIRK channels and GABA(B) receptors with excitatory synapses at both post- and pre-synaptic sites indicates their intimate involvement in the modulation of glutamatergic neurotransmission in the cerebellum. PMID: 19558451
    23. two spermine molecules bind to a tetramer of Kir3.1/GIRK1 with a dissociation constant of 26 microM, which is lower than other blockers. PMID: 19620244
    24. The s present a crystal structure of the cytoplasmic portion of a Kir channel with five ions bound and demonstrate that a constriction near the intracellular end of the pore, acting as a gasket, prevents K(+) ions from bypassing the blocker. PMID: 19915587

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ3 subfamily
  • 数据库链接：

    KEGG: mmu:16519

    STRING: 10090.ENSMUSP00000063329

    UniGene: Mm.418451