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  4. Recombinant Mouse Fractalkine (Cx3cl1)

Recombinant Mouse Fractalkine (Cx3cl1)

  • 货号：
    CSB-CF006235MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Cx3cl1; Cx3c; Fkn; Scyd1; Fractalkine; FK; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-395
  • 氨基酸序列
    QHLGMTKCEIMCDKMTSRIPVALLIRYQLNQESCGKRAIVLETTQHRRFCADPKEKWVQDAMKHLDHQAAALTKNGGKFEKRVDNVTPGITLATRGLSPSALTKPESATLEDLALELTTISQEARGTMGTSQEPPAAVTGSSLSTSEAQDAGLTAKPQSIGSFEAADISTTVWPSPAVYQSGSSSWAEEKATESPSTTAPSPQVSTTSPSTPEENVGSEGQPPWVQGQDLSPEKSLGSEEINPVHTDNFQERGPGNTVHPSVAPISSEETPSPELVASGSQAPKIEEPIHATADPQKLSVLITPVPDTQAATRRQAVGLLAFLGLLFCLGVAMFAYQSLQGCPRKMAGEMVEGLRYVPRSCGSNSYVLVPV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1.; The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.; The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.
  • 基因功能参考文献：
    1. The data of this study suggested that overexpression of only chemokine domain of CX3CL1 does not protect against tau pathology. PMID: 30253780
    2. the CX3CL1/CX3CR1 axis contributes to the proliferative and pro-inflammatory effects of Ang II in VSMCs. PMID: 29356931
    3. that CX3CL1-CX3CR1 signaling is a molecular mechanism capable of modulating microglial-mediated degeneration PMID: 27314452
    4. CXCR4(+) CD45(-) bone marrow cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow. PMID: 27339271
    5. Increased fractalkine and its receptor CX3CR1 may cause a cross-talk between activated glial cells and neurons, playing an important role in the development of neuroinflammation in fructose-fed mice. PMID: 26765996
    6. miR-223 controls the expression of CX3CL1 by targeting HDAC2 in chronic obstructive pulmonary disease patients and mouse models of the disease. PMID: 26864305
    7. in this study, CX3CL1 is identified as a novel substrate of MMP-19 PMID: 26555704
    8. The CX3CL1/CX3CR1 system is essential for restricting coxsackievirus B3-induced myocarditis. PMID: 28800592
    9. changes in GSK-3beta activity and/or levels regulate the production and subsequent secretion of fractalkine, a chemokine involved in the immune response that has been linked to AD and to other different neurological disorders. PMID: 27832289
    10. Medial ganglionic eminence (MGE) interneurons secrete fractalkine that promotes genesis of oligodendrocytes from glially biased cortical precursors in culture. Moreover, when MGE interneurons are genetically ablated in vivo prior to their migration, this causes a deficit in cortical oligodendrogenesis. PMID: 28472653
    11. our findings reveal a previously unknown regulatory role for LRRK2 in CX3CR1 signalling and suggest that an increase of CX3CR1 activity contributes to the attenuated inflammatory responses in Lrrk2-null microglia PMID: 27378696
    12. a crucial role of CX3CL1-CX3CR1 in experimental colitis, in particular for intestinal leukocyte recruitment during murine colitis, is reported. PMID: 27942903
    13. These results strongly suggest the involvement of CX3CL1 in the migration of osteoclast precursors and osteoclastogenesis. PMID: 27579490
    14. s provide evidence that interactions between CX3CL1 and CX3CR1 play crucial roles in determining the number of M1 macrophages within the skin of mice, which in turn can have dramatic effects on psoriasis-like inflammation. PMID: 26976687
    15. In the absence of the rd8 allele, deficiency of CCR2 and CX3CL1 in mice leads to a mild form of retinal degeneration which is associated with the recruitment of macrophages, particularly to the subretinal space. This model enables to assess consequences of perturbed chemokine signaling, but it does not recapitulate cardinal age-related macular degeneration features. PMID: 26670885
    16. Also icariin reduced CX3CR1 and CX3CL1 protein levels in the artery wall. In conclusion, icariin could be a potential anti-atherosclerosis agent by downregulating the expression of CX3CR1. PMID: 26802470
    17. The biological activity of CX3CL1 is regulated by conversion of a membrane integrated to a soluble form during neurogenesis and in response to pathologic changes in the adult retinal milieu. PMID: 25191897
    18. CX3CL1/CX3CR1 signaling is involved in LTP of C-fiber-evoked field potentials in the rodent spinal dorsal horn PMID: 25768734
    19. CX3CL1/CX3CR1-mediated microglial activation plays a detrimental role in ischemic brain via p38MAPK/PKC signaling PMID: 25966946
    20. Cx3cl1 overexpression suppresses alpha-synuclein-mediated neurodegeneration. PMID: 25195598
    21. IFN-gamma induces aberrant CD49b+ NK cell recruitment and pregnancy failure through regulating CX3CL1. PMID: 25375377
    22. Insulin resistance increases plaque vulnerability by augmenting the CX3CL1/CX3CR1 axis, which is mechanistically linked to reduced vascular smooth muscle cell survival PMID: 24788416
    23. Together, these studies challenge the "frustrated phagocytosis" concept and suggest that neuronal-microglial communication link the two central AD pathologies. PMID: 25209291
    24. CX3CL1 may contribute to the regulation of toxigenic C. difficile infection. PMID: 24362517
    25. These results demonstrate that the de novo CX3CL1-CX3CR1 axis plays a pivotal role in osteoclast recruitment and subsequent bone resorption PMID: 24401612
    26. our data suggest that the CX3CR1/ CX3CL1 pathway is involved in the recruitment of circulating CD16 thorn CX3CR1 thorn monocytes to the periprosthetic tissues. PMID: 24700421
    27. Data indicate that atopic dermatitis (AD) pathology and immune responses were profoundly decreased in CX3CR1-deficient mice and upon blocking CX3CL1-CX3CR1 interactions in wild-type mice. PMID: 24821910
    28. Loss of ACE2 exacerbates AngII-mediated inflammation, myocardial injury and dysfunction in ACE2-deficient hearts via activation of the CTGF-FKN-ERK and MMP signaling. PMID: 24161906
    29. CX3CL1 transiently potentiates NMDAR function though mechanisms involving A2AR activity and the release of D-serine. PMID: 23981568
    30. Interactions between CX3CL1 and CX3CR1 may contribute to the development of leukocytoclastic vasculitis. PMID: 23470165
    31. HDACs and NF-kB signaling coordinate epithelial expression of CX3CL1 to promote mucosal antimicrobial defense through suppression of the mir-424-503 gene. PMID: 23724129
    32. carotid artery injury was associated with greater chemokine 1 CX3C expression in the acute phase followed by greater CX3C receptor 1 coexpressing smooth muscle-like cell content in later lesions and less neointima formation than in femoral arteries PMID: 23653073
    33. our data indicate an upregulation of fractalkine and downregulation of CX3CR1 in sepsis, which seems to be mediated by the transcripting factor NF-KappaB likely via reduced liberation of proinflammtory cytokines in the whole murine organism. PMID: 23026294
    34. Extracellular adenosine is an endogenous modulator of neuroinflammation that induces CX3CL1 at the chorid plexus. PMID: 22883932
    35. Fractalkine's essential role in the formation of atherosclerotic lesions and atherosclerosis progression has been impressively described in mouse models. Review. PMID: 22739755
    36. Suggest that Ang-II induces functional CX(3)CL1 expression in arterial but not in venous endothelial cells. PMID: 23117657
    37. H(2)S hampers the progression of atherosclerosis in fat-fed apoE(-/-) mice and downregulates CX3CR1 and CX3CL1 expression on macrophages and in lesion plaques PMID: 22815945
    38. Hypoxic release of endothelial CX3CL1 induced SMC phenotypic switching from the contractile to the proliferative state. Inhibition of CX3CR1 prevented CX3CL1 stimulation of SMC proliferation and monolayer expansion. PMID: 23002075
    39. Fractalkine is expressed in early and advanced atherosclerotic lesions and supports monocyte recruitment via CX3CR1. PMID: 22916279
    40. Fractalkine induced cellular reactive oxygen species production and activation of ERK1/2 and p38 MAPK in mesangial cells, stimulating cell proliferation. PMID: 22564616
    41. Lipopolysaccharide induces monocyte-mesangial cell binding through the fractalkine/CX3CR1 system. PMID: 22564617
    42. findings that exogenous fractalkine reduces microglial motility and fails to protect neurons co-cultured with Cx3cr1-/- mixed glia suggest that fractalkine may act by interfering with toxic microglial-neuron interactions PMID: 22093090
    43. Fractalkine promotes myocardial injury and accelerates the progress of heart failure, which is associated with the activation of MAPKs. PMID: 21840883
    44. Structure/function and expression analysis of the CX3C chemokine fractalkine. PMID: 21951685
    45. fractalkine-CX3CR1 axis contributes to kidney fibrosis in a hypertensive mouse model. PMID: 21451526
    46. Syk mediated chemotaxis toward CX3CL1 by regulating both Rac1/WAVE2 and Cdc42/WASP pathways, whereas Src family kinases were required for proper WASP tyrosine phosphorylation. PMID: 21388954
    47. Fractalkine attenuates excito-neurotoxicity via microglial clearance of damaged neurons and antioxidant enzyme heme oxygenase-1 expression PMID: 21071446
    48. CX3CR1-expressing macrophages are induced by CX3CL1/fractalkine to express heme oxygenase-1, thereby ameliorating Clostridium difficile toxin A-induced enteritis. PMID: 21131421
    49. Fractalkine-induced depression of excitatory postsynaptic current is absent in cultures from adenosine A3 receptor-deficient mice but not in tissue from adenosine A1- or A2-receptor-deficient mice. PMID: 20570369
    50. The CX3CL1-CX3CR1 interaction inhibits inflammatory properties in Kupffer cells/macrophages and results in decreased liver inflammation and fibrosis. PMID: 20683935

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.; [Processed fractalkine]: Secreted.
  • 蛋白家族：
    Intercrine delta family
  • 组织特异性：
    Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis. Highly expressed in lesional smooth muscle cells, but not macrophages.
  • 数据库链接：

    KEGG: mmu:20312

    STRING: 10090.ENSMUSP00000034230

    UniGene: Mm.103711