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  4. Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 1 (Ceacam1)

Recombinant Mouse Carcinoembryonic antigen-related cell adhesion molecule 1 (Ceacam1)

  • 货号：
    CSB-CF005157MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ceacam1; Bgp; Bgp1; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; Biliary glycoprotein D; MHVR1; Murine hepatitis virus receptor; MHV-R; CD antigen CD66a
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    35-521
  • 氨基酸序列
    EVTIEAVPPQVAEDNNVLLLVHNLPLALGAFAWYKGNTTAIDKEIARFVPNSNMNFTGQAYSGREIIYSNGSLLFQMITMKDMGVYTLDMTDENYRRTQATVRFHVHPILLKPNITSNNSNPVEGDDSVSLTCDSYTDPDNINYLWSRNGESLSEGDRLKLSEGNRTLTLLNVTRNDTGPYVCETRNPVSVNRSDPFSLNIIYGPDTPIISPSDIYLHPGSNLNLSCHAASNPPAQYFWLINEKPHASSQELFIPNITTNNSGTYTCFVNNSVTGLSRTTVKNITVLEPVTQPFLQVTNTTVKELDSVTLTCLSNDIGANIQWLFNSQSLQLTERMTLSQNNSILRIDPIKREDAGEYQCEISNPVSVRRSNSIKLDIIFDPTQGGLSDGAIAGIVIGVVAGVALIAGLAYFLYSRKSGGGSDQRDLTEHKPSTSNHNLAPSDNSPNKVDDVAYTVLNFNSQQPNRPTSAPSSPRATETVYSEVKKK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis. Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T-cells, natural killer (NK) and neutrophils. Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70. Also inhibits T-cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T-cell through its interaction with HAVCR2. Upon natural killer (NK) cells activation, inhibit KLRK1-mediated cytolysis of CEACAM1-bearing tumor cells by trans-homophilic interactions with CEACAM1 on the target cell and lead to cis-interaction between CEACAM1 and KLRK1, allowing PTPN6 recruitment and then VAV1 dephosphorylation. Upon neutrophils activation negatively regulates IL1B production by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, that dephosphorylates SYK, reducing the production of reactive oxygen species (ROS) and lysosome disruption, which in turn, reduces the activity of the inflammasome. Downregulates neutrophil production by acting as a coinhibitory receptor for CSF3R by downregulating the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R. Also regulates insulin action by promoting INS clearance and regulating lipogenesis in liver through regulating insulin signaling. Upon INS stimulation, undergoes phosphorylation by INSR leading to INS clearance by increasing receptor-mediated insulin endocytosis. This inernalization promotes interaction with FASN leading to receptor-mediated insulin degradation and to reduction of FASN activity leading to negative regulation of fatty acid synthesis. INSR-mediated phosphorylation also provokes a down-regulation of cell proliferation through SHC1 interaction resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways. Functions as activator in angiogenesis by promoting blood vessel remodeling through endothelial cell differentiation and migration and in arteriogenesis by increasing the number of collateral arteries and collateral vessel calibers after ischemia. Also regulates vascular permeability through the VEGFR2 signaling pathway resulting in control of nitric oxide production. Downregulates cell growth in response to EGF through its interaction with SHC1 that mediates interaction with EGFR resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 pathway. Negatively regulates platelet aggregation by decreasing platelet adhesion on type I collagen through the GPVI-FcRgamma complex. Inhibits cell migration and cell scattering through interaction with FLNA; interfers with the interaction of FLNA with RALA. Mediates bile acid transport activity in a phosphorylation dependent manner. Negatively regulates osteoclastogenesis.; Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Promotes populations of T-cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens.; (Microbial infection) In case of murine coronavirus (MHV) infection, serves as receptor for MHV S1 spike glycoprotein.
  • 基因功能参考文献：
    1. regulator of local immune response in the liver, whereby CEACAM1S seems to control regulatory T cell induction PMID: 29377208
    2. CEACAM1 controls epithelial-mesenchymal transition in vitro and in vivo by site-specific regulation of beta-catenin phosphorylation PMID: 27572314
    3. A 30-day high fat feeding regimen demonstrated that white adipose tissue-derived fatty acids repressed hepatic CEACAM1-dependent regulation of insulin and lipid metabolism in 3-month-old male C57BL/6J mice. PMID: 27777319
    4. determined the crystal structure of mCEACAM1b and identified the structural differences and additional residue differences between mCEACAM1a and mCEACAM1b that affect MHV binding and entry PMID: 28035001
    5. REVIEW: summarizes the vascular effects of CEACAM1 and focuses on its role in vascular morphogenesis and endothelial barrier regulation PMID: 27695943
    6. CEACAM1 exacerbates hypoxic cardiomyocyte injury and post-infarction cardiac remodeling by enhancing cardiomyocyte mitochondrial dysfunction and endoplasmic reticulum stress-induced apoptosis. PMID: 26911181
    7. The increase in total fat mass in Cc1(-/-) mice is mainly attributed to hyperphagia and reduced spontaneous physical activity. Although the contribution of the loss of CEACAM1 from anorexigenic proopiomelanocortin neurons in the arcuate nucleus is unclear, leptin resistance and elevated hypothalamic fatty-acid synthase activity could underlie altered energy balance in these mice. PMID: 27002145
    8. Ceacam1 is essential for normal integrin aIIbb3-mediated platelet function; the disruption of mouse Ceacam1 induced moderate integrin aIIbb3-mediated functional defects. PMID: 26196244
    9. hepatic CEACAM1 expression at fasting is mediated by Pparalpha-dependent mechanisms. PMID: 26846848
    10. Gram-positive bacteria promote the mRNA expression of CEACAM1 or CEACAM20 in the small intestine. Inflammatory cytokines or butyrate likely participates in such effects of commensal bacteria. PMID: 25908210
    11. CEACAM1 is a crucial regulator of B-cell survival, influencing B-cell numbers and protective antiviral antibody responses PMID: 25692415
    12. High-fat diet amplifies the permissive effect of Ceacam1 deletion on renal expression of all renin-angiotensin system components, PI3K phosphorylation, inflammation, and fibrosis. PMID: 26374765
    13. Ceacam1L acts as a crucial factor in glioblastoma-initiating cell maintenance and tumorigenesis by activating c-Src/STAT3 signaling. Monomers of the cytoplasmic domain of Ceacam1L bound to c-Src and STAT3 and induced their phosphorylation. PMID: 26238781
    14. CEACAM1 levels in the liver were reduced in prenatally stressed diet-induced obesity offspring after the high fat diet challenge, suggesting that preexisting genetic predisposition combined with prenatal stress increases the risk for obesity in adulthood. PMID: 26219866
    15. high-fat diet reduced hepatic CEACAM1 expression and that overexpressing CEACAM1 in liver curtailed diet-induced metabolic abnormalities by protecting hepatic insulin clearance. PMID: 25972571
    16. a physiologic role of CEACAM1 in the regulation of osteoclastogenesis PMID: 25490771
    17. CD66a and CD117 may be useful markers to isolate several cell types consisting of SMG epithelium and to analyze their molecular and cellular nature. PMID: 25498293
    18. expression significantly reduced in the colon tissue of mice with ulcerative colitis PMID: 25724769
    19. This is the first study demonstrating that CEACAM1 enhances vascular remodeling and tuft regression by increasing endothelial resistance to alterations in oxygen tension, thus accelerating vascular recovery after systemic hypoxia. PMID: 25406283
    20. CEACAM1 serves as a heterophilic ligand for TIM-3 that is required for its ability to mediate T-cell inhibition, and this interaction has a crucial role in regulating autoimmunity and anti-tumour immunity PMID: 25363763
    21. CEACAM1 controls matrix metalloproteinase-9 secretion by neutrophils in postischemic inflammation at the blood brain barrier after stroke. PMID: 23780386
    22. CEACAM1 on activated NK cells functions as an inhibitory receptor for NKG2D-mediated cytolysis, which has important implications for understanding the means by which CEACAM1 expression adversely affects tumor immunity. PMID: 23696226
    23. These results suggest that CEACAM1 has roles in the initiation of palatal fusion via epithelial cell adhesion. PMID: 23613893
    24. Data from knockout mice suggest that Ceacam1 plays role in regulation of vascular endothelial reactions (such as nitric oxide production) to oxidative stress/lipid peroxidation and prevention of plaque-like lesions in large vessels such as aorta. PMID: 23800882
    25. global null deletion of Ceacam1 caused an increase in blood pressure with increased renin-angiotensin system activation together with upregulation of prorenin receptor via PI3K-Akt activation of CREB-1, ATF-1, ATF-2, and NF-kappaB p65. PMID: 23734002
    26. PKC-epsilon can affect insulin uptake in mouse embryonic fibroblasts through promotion of receptor-mediated endocytosis, and that this may be mediated by regulation of CEACAM1 expression. PMID: 23469261
    27. Carcinoembryonic antigen cell adhesion molecule 1 promotes metastasis of colorectal cancer. PMID: 22469976
    28. This tissue resident predominance of CEACAM1-S expression was determined by the intestinal environment. PMID: 23123061
    29. CEACAM1 expression on CD11b(+)/Gr-1(+) myeloid cells is a prerequisite for adequate collateral formation. PMID: 22962327
    30. Neither of the two CEACAM1 isoforms defines hepatitis virus organ tropism. PMID: 22673933
    31. Findings show that CEACAM1 negatively regulates Gr1(+)CD11b(+) myeloid cell-dependent tumor angiogenesis by inhibiting the G-CSF-Bv8 signaling pathway. PMID: 22406619
    32. CEACAM specific T84.1 antibody binds to tumour cells in the vicinity of blood vessels PMID: 22162753
    33. CEACAM1 dampens antitumor immunity by down-regulating NKG2D ligand expression on tumor cells. PMID: 22143889
    34. Data show that CEACAM1 expression in the tumor periphery determines the vascular phenotype in a tumor, whereas systemic absence of CEACAM1 interferes with the formation of an organized tumor matrix and intratumoral vessel maturation. PMID: 21532628
    35. Ceacam1 was studied as a regulator of graft-versus-host-disease and graft-versus-tumor after allogeneic bone marrow transplantation (allo-BMT) in mouse models. PMID: 21760897
    36. This data represents the first report of a functional link between CEACAM1 and the VEGFR2/Akt/eNOS-mediated vascular permeability pathway. PMID: 21081647
    37. CEACAM1 acted as a coinhibitory receptor for G-CSFR regulating granulopoiesis and host innate immune response to bacterial infections. PMID: 21029969
    38. ceacam1a mRNA expression was quantified in murine tissues and primary cells; ability of virus to spread to adjacent cells in ceacam1a knockout mice was species dependent PMID: 20739537
    39. These results strongly support the hypothesis that although alleles of mCEACAM1 are the principal determinants of mouse susceptibility to mouse hepatitis virus -A59, other as-yet-unidentified murine genes may also play a role. PMID: 20410265
    40. In contrast to epithelial cells and other cell types, CEACAM1 expression on the cell surface of mouse T cells is revealed only upon activation and requires neither de novo transcription or translation. PMID: 11801635
    41. regulates insulin clearance in liver PMID: 11850617
    42. Both long and short cytoplasmic domain isoforms of Ceacam1 are expressed in mouse neutrophils, B cells, and T-cells. PMID: 11994468
    43. homophilic CEACAM1-CEACAM1 cell-mediated binding is the physiological stimulus for CEACAM1-triggered B cell signaling PMID: 12832451
    44. the transmembrane domain of CEACAM1 is responsible for the Cdc42-induced targeting at cell-cell contacts PMID: 14517298
    45. CD98 stimulation by anti-CD98 antibodies induced CEA-CAM-1-mediated cell adhesion PMID: 14527684
    46. CEACAM1 isoforms are a novel class of activation-induced cell surface molecules on T cells that function in the specific regulation of Th1-mediated inflammation. PMID: 14970176
    47. expression on microglia was reduced during acute Murine Hepatitis virus infection and restored following immune-mediated virus control PMID: 15220458
    48. CEACAM1 has a role in insulin clearance and regulating serum free fatty acid (FFA) levels PMID: 15316023
    49. CEACAM1a is the sole receptor for mouse hepatitis virus A59 in both liver and brain, and its deletion from the mouse renders the mouse completely resistant to infection by this virus. PMID: 15331748
    50. Data show that CEACAM1 is a substrate of the epidermal growth factor receptor (EGFR) and that upon phosphorylation, CEACAM1 reduces EGFR-mediated growth of transfected Cos-7 and MCF-7 cells in response to EGF. PMID: 15467833

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Lateral cell membrane. Apical cell membrane. Basal cell membrane. Cell junction. Cell junction, adherens junction.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein. Lateral cell membrane. Apical cell membrane. Basal cell membrane. Cell junction. Cell junction, adherens junction. Cytoplasmic vesicle, secretory vesicle.; Cell projection, microvillus membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, CEA family
  • 组织特异性：
    Expressed in granulocytes, lymphocytes, granulocytes, B cells, and T-cells. Expressed in bone. Highly expressed in liver and femur. Highly expressed in neutrophils, and to a lesser extent inmonocytes, and macrophages. Slightly higher expressed in peripher
  • 数据库链接：

    KEGG: mmu:26365

    STRING: 10090.ENSMUSP00000096266

    UniGene: Mm.322502