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  4. Recombinant Mouse Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (Lfng)

Recombinant Mouse Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (Lfng)

  • 货号：
    CSB-CF012881MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Lfng; Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-378
  • 氨基酸序列
    MLQRCGRRLLLALVGALLACLLVLTADPPPTPMPAERGRRALRSLAGSSGGAPASGSRAAVDPGVLTREVHSLSEYFSLLTRARRDADPPPGVASRQGDGHPRPPAEVLSPRDVFIAVKTTRKFHRARLDLLFETWISRHKEMTFIFTDGEDEALAKLTGNVVLTNCSSAHSRQALSCKMAVEYDRFIESGKKWFCHVDDDNYVNLRALLRLLASYPHTQDVYIGKPSLDRPIQATERISEHKVRPVHFWFATGGAGFCISRGLALKMGPWASGGHFMSTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTTELHEQVTLSYGMFENKRNAVHIKGPFSVEADPSRFRSVHCHLYPDTPWCPRSAIF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1. Decreases the binding of JAG1 to NOTCH2 but not that of DLL1. Essential mediator of somite segmentation and patterning. During somite boundary formation, it restricts Notch activity in the presomitic mesoderm to a boundary-forming territory in the posterior half of the prospective somite. In this region, Notch function activates a set of genes that are involved in boundary formation and in anterior-posterior somite identity. Ectopically expressed in the thymus, Lfgn inhibits Notch signaling which results in inhibition of T-cell commitment and promotes B-cell development in lymphoid progenitors. May play a role in boundary formation of the enamel knot.
  • 基因功能参考文献：
    1. results suggest that mir-125a sites in the Lfng 3' untranslated region influence transcript turnover in both mouse and chicken embryos, and support the existence of position-dependent regulatory mechanisms in the presomitic mesoderm. PMID: 28710810
    2. Lfng-mediated Notch signaling appears to be a key factor governing NSC quiescence, division, and fate. PMID: 28699891
    3. These results show that Notch signaling is finely calibrated in the cochlea via Lfng and Mfng to produce precisely tuned levels of signaling that first set the boundary of the organ of Corti and later regulate hair cell development. PMID: 27966429
    4. LFNG protein may have context-dependent effects on Notch activity; somitogenesis is disrupted by a novel dominant allele of Lfng PMID: 26811377
    5. each Fringe contributes to T and B cell development, and Fringe is required for optimal in vitro stimulation of T and B cells. PMID: 26608918
    6. Lfng expression and activity is normal in mice whose Lfng is lengthened by 10 kb, and no effects on segmentation are evident. PMID: 22326607
    7. suggest that modulation of the Notch signaling by Lfng affects the clock period during development PMID: 24560643
    8. STAT5-dependent amplification of Notch-modifying Lfng augments Th2 response via Dll4 and is critical for amplifying viral exacerbation during allergic airway disease. PMID: 24367028
    9. The repressive effect of Lfng against Notch activities in neighbouring cells can sufficiently explain the synchronization in vivo. PMID: 23072809
    10. Intriguing changes are observed in the cranio-caudal borders of multifidus muscle in mutant Dll3 and Lfng models of idiopathic scoliosis. PMID: 22095884
    11. Reduced LFNG expression facilitates JAG/NOTCH luminal progenitor signaling and cooperates with MET/CAVEOLIN basal-type signaling to promote basal-like breast cancer. PMID: 22624713
    12. the presence of Gal on O-fucose glycans differentially affects DLL1-induced NOTCH signaling modulated by LFNG versus MFNG PMID: 22081605
    13. Decreasing Lfng expression during the (TCR-CD4/CD8 double negative 3) DN3-DP (CD4/CD8 double positive) transition minimizes the potent leukemogenic potential of Notch1 signaling. PMID: 21097675
    14. data reveal that Lfng enhances CD4/CD8 double-negative 3b precursor competition for intrathymic Delta-like Notch ligands to maximize Notch-induced clonal expansion during the earliest stage of beta-selection. PMID: 20844195
    15. Data suggest that lunatic fringe strongly augments Notch signaling mediated by Delta-like 1 but not Jagged 1. PMID: 20510365
    16. there are differential axial requirements for lunatic fringe and Hes7 transcription during mouse somitogenesis PMID: 19956724
    17. An evolutionarily conserved region in murine Lunatic fringe (Lfng) promoter drives periodic somite expression. Oscillatory Notch signaling underlies the segmentation clock and directly activates and indirectly represses Lfng expression. PMID: 12110168
    18. Lunatic fringe (Lfng) expression in the presomitic mesoderm is required for somite segmentation. Independent Lfng regulatory cassettes advance a molecular framework for deciphering this segmentation. PMID: 12110169
    19. Lunatic fringe, FGF, and BMP regulate the Notch pathway during epithelial morphogenesis of teeth. PMID: 12167404
    20. Transcriptional oscillation of this protein is essential for somitogenesis. PMID: 12670869
    21. periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock PMID: 12783854
    22. Lunatic fringe null female mice were found to be infertile. PMID: 15659488
    23. The Jsr mutation disrupts the A-P polarity of somites during the somitogenesis without altering Lfng expression pattern in the presomitic mesoderm. PMID: 15822856
    24. Lunatic fringe and Hes7 controls their oscillatory expression during somitogenesis by forming negative feedback loop PMID: 16342160
    25. The ability of Lfng and Notch1 to control progenitor competition for limiting cortical niches is an important mechanism for the homeostatic regulation of thymus size. PMID: 16699526
    26. Shutting down the expression of Lfng in CD4+CD8+DP cells may have a physiological role in promoting DP cells differentiation toward mature CD8+SP cells. PMID: 17142733
    27. spondylocostal dysostosis (SCD) is caused by mutation in Delta-like 3 (DLL3), Mesoderm posterior 2 (MESP2), and Lunatic fringe (LFNG); three genes that are components of the Notch signaling pathway. PMID: 17600782
    28. In the absence of oscillatory Lfng expression, mice exhibit severe segmentation phenotypes in the thoracic and lumbar skeleton PMID: 18234727
    29. Lunatic Fringe (Lfng) and Manic Fringe (Mfng) cooperatively enhanced the DL1-Notch2 interaction to promote marginal zone B cell development PMID: 19217325
    30. The expression of lunatic fringe oscillates in 2-h cycles in the mouse presomitic mesoder. PMID: 19440349
    31. No obvious synergistic defects in mice in the absence of two, or all three, fringe genes during development of the axial skeleton, limbs, hindbrain, and cranial nerves. PMID: 19479951
    32. Lfng functions to enhance Notch signaling in myofibroblast precursor cells and thereby to coordinate differentiation and mobilization of myofibroblasts required for alveolar septation. PMID: 19897741

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  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyltransferase 31 family
  • 组织特异性：
    Detected at 12.5 dpc in all tissues examined with the highest level observed in adult brain and spleen. Detected in the dental epithelium.
  • 数据库链接：

    KEGG: mmu:16848

    STRING: 10090.ENSMUSP00000031555

    UniGene: Mm.12834