Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 跨膜蛋白
  4. Recombinant Mouse B-lymphocyte antigen CD19 (Cd19)

Recombinant Mouse B-lymphocyte antigen CD19 (Cd19)

  • 货号：
    CSB-CF004888MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Cd19; B-lymphocyte antigen CD19; Differentiation antigen CD19; CD antigen CD19
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-547
  • 氨基酸序列
    RPQKSLLVEVEEGGNVVLPCLPDSSPVSSEKLAWYRGNQSTPFLELSPGSPGLGLHVGSLGILLVIVNVSDHMGGFYLCQKRPPFKDIWQPAWTVNVEDSGEMFRWNASDVRDLDCDLRNRSSGSHRSTSGSQLYVWAKDHPKVWGTKPVCAPRGSSLNQSLINQDLTVAPGSTLWLSCGVPPVPVAKGSISWTHVHPRRPNVSLLSLSLGGEHPVREMWVWGSLLLLPQATALDEGTYYCLRGNLTIERHVKVIARSAVWLWLLRTGGWIVPVVTLVYVIFCMVSLVAFLYCQRAFILRRKRKRMTDPARRFFKVTPPSGNGTQNQYGNVLSLPTSTSGQAHAQRWAAGLGSVPGSYGNPRIQVQDTGAQSHETGLEEEGEAYEEPDSEEGSEFYENDSNLGQDQVSQDGSGYENPEDEPMGPEEEDSFSNAESYENADEELAQPVGRMMDFLSPHGSAWDPSREASSLGSQSYEDMRGILYAAPQLHSIQSGPSHEEDADSYENMDKSDDLEPAWEGEGHMGTWGTT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.
  • 基因功能参考文献：
    1. The results suggest that CD19 controls the differentiation of marginal zone precursors to marginal zone B cells by regulating ADAM28-mediated Notch2 cleavage. PMID: 28707394
    2. This study demonstrates that antiviral protective antibody-secreting cells (ASC) in the central nervous system are dependent on CD19 activation and peripheral Germinal Center formation, while accumulation of early-recruited IgD(+) B cells is CD19 independent. PMID: 28931676
    3. this study shows that mitochondrial reactive oxygen species suppress humoral immune responses through reduction of CD19 expression and resultant B cell receptor signaling in B cells PMID: 27883180
    4. autoimmunity induced by excessive BAFF production requires B1b B cells and CD19 signaling. PMID: 26103922
    5. WIPF1 deficiency impaired CD19 co-receptor activation and subsequent PI3 kinase signaling, by distorting the actin and tetraspanin networks that lead to altered CD19 cell surface dynamics. PMID: 26453379
    6. Syk-deficient B cells require BAFF receptor and CD19/PI3K signaling for their long-term survival. PMID: 25630702
    7. The selection of mature B cells is critically dependent on the expression level of the co-receptor CD19. PMID: 24472602
    8. This inhibitory function of FcgammaRIIB in impairing the spatial-temporal colocalization of BCR and CD19 microclusters in the B cell immunological synapse may help explain the hyper-reactive features of systemic lupus erythematosus PMID: 24790152
    9. These results show that the ability of CD19-CAR T-cells to home in on tumor lesions is pivotal for their anti-tumor effects in our xenograft models, and therefore may enhance the efficacy of adoptive T-cell therapy for refractory B-cell lymphoma. PMID: 23872144
    10. Data show that effective B cell receptor (BCR) signaling requires collaboration with the coreceptor CD19 organized by the CD81-tetraspanin network. PMID: 23499492
    11. results indicate that the CD19/CD81 complex interacts with CD38 but this interaction is not required to induce proliferation in mouse B lymphocytes PMID: 22564057
    12. CD19positive CD45R(lo)positive lymphocytes of embryonic origin are present in Peyer's patches and in the spleen throughout the life span of wild-type mice, beginning at postnatal day 7. PMID: 22837485
    13. We report that regulatory dendritic cells can induce splenic B cells to differentiate into a distinct subtype of IL-10-producing regulatory B cells with unique phenotype CD19(hi)FcgammaIIb(hi). PMID: 22692512
    14. CD19 is a major BCR-independent regulator of MYC-driven neoplastic growth in B cell neoplasms. PMID: 22546857
    15. Data from transgenic/knockout mice indicate that CD19 is not required for generation of marginal zone B-cells under influence of Notch2 signaling; data confirm that in absence of CD19, all B cells shift to marginal zone B-cell compartment. PMID: 21795747
    16. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes CD19 can eradicate lymphoma and normal B cells. PMID: 20631379
    17. CD19 expression has both protective and disease promoting roles in lupus pathogenesis in New Zealand black (NZB) and in New Zealand white F1 hybrid (NZB/W) mice deficient for CD19 (CD19-/-). PMID: 20368271
    18. BCR engagement induced differential Tyr phosphorylation. Y(513) phophorylation occurred 1st; Y(482) phosphorylation was delayed & transient. This affected CD19-mediated downstream pathways. Phosphorylation differed with the plasma membrane location. PMID: 20101619
    19. Ca(2+) down-regulates SLC and CD19 gene expression upon pre-BCR activation through inhibition of E2A by Ca(2+)/calmodulin. PMID: 20022378
    20. CD19-dependent B lymphocyte signaling thresholds influence skin fibrosis and autoimmunity in the tight-skin mouse PMID: 12045259
    21. The physiologic role of eight CD19 tyrosines was examined in CD19-knockout mice expressing transgenic CD19 constructs. PMID: 12387743
    22. The CD19 B-cell signaling pathway is required for replication of the LP-BM5 mixture of murine leukemia viruses in genetically susceptible mice and subsequent development of lymphadenopathy and immunodeficiency. PMID: 12421939
    23. CD19 phosphorylation after B cell receptor ligation is found to be unaffected by the absence of Lyn, yet dependent on Src family protein tyrosine kinases, as it is inhibited fully by an Src family-specific inhibitor. PMID: 12471124
    24. Modulation of cell survival is a key property of the function of the B cell coreceptor CD19 in vivo and accounts for some of the phenotypes observed in CD19-/- knockout mice. PMID: 12496385
    25. CD19 contributes to B cell generation at the early antigen-independent stages of B cell development, in addition to important roles in the peripheral immune system. PMID: 14634103
    26. editing, immature 3-83Tg B cells deficient of CD19 have elevated BCR tonic signals and that lowering these tonic signals effectively suppresses receptor editing. PMID: 14668327
    27. CD19 has an important function in regulating positive selection and maturation of nontransgenic B-cell precursors PMID: 15644414
    28. CD19(-/-) mice generated B-1b cells and protective adaptive PPS-specific antibody responses, whereas hCD19Tg mice lacked B-1b cells and adaptive PPS-specific antibody responses. PMID: 16039575
    29. Susceptibility to collagen-induced arthritis requires CD19 on B cells. PMID: 16210644
    30. CD19-/- mice have a marked loss of peritoneal B-1 cells, hyporesponsive to mitogens and BCR ligation. PMID: 16227086
    31. streptavidin-C3dg enhancement of BCR-induced [Ca2+]i responses required CD21 and CD19 expression and resulted in significantly enhanced CD19 and Lyn phosphorylation, with enhanced Lyn/CD19 associations PMID: 16339538
    32. These findings show that Vav1 and PI3K regulation of PLCgamma contributes to the activation of PKD in response to BCR and or CD19 cross-linking. PMID: 16380231
    33. CD19 modulates the Th1/Th2 cytokine balance in B cells and plays a critical role as a suppressive molecule in the development of experimental autoimmune encephalomyelitis. PMID: 16507897
    34. requirements of PI3K subunits for BCR or BCR/CD19-induced ERK activation PMID: 16802598
    35. in-vivo experiments using anti-CD19 immunotoxin (IT) with vincristine prolonged the survival of mice PMID: 17107913
    36. CD19 expression in B cells is critical for termination of contact hypersensitivity responses, possibly through the function of regulatory B cells. PMID: 17556590
    37. CD19 may be the signaling intermediate in B cells activated by CD86 proximal to phosphatidylinositol 3-kinase (PI3K). PMID: 17641017
    38. BCAP and CD19 have complementary roles in BCR-mediated phosphoinositide 3-kinase activation PMID: 18025150
    39. There is an essential role for CD19, independent of the costimulatory molecule CD21, in amplifying early B cell activation events in response to membrane-bound ligand stimulation. PMID: 18059271
    40. CD19 deficiency suppresses fibrosis and autoantibody production by inhibiting TLR4 signals. PMID: 18467694
    41. identified a B cell-specific upstream enhancer and showed that the developmental regulation of Cd19 expression involves precisely coordinated alterations in transcription factor binding and chromatin remodeling at Cd19 cis-regulatory elements. PMID: 18552207
    42. NK cells in the CD19- B220+ bone marrow fraction are increased in senescence and reduce E2A and surrogate light chain proteins in B cell precursors. PMID: 19428458
    43. deficiency of marginal zone B cells in CD19(-/-) mice is caused by a defect of intrinsic B cell signaling PMID: 19494255
    44. Findings suggest a defect in CD19-mediated signals in both peritoneal and splenic B-1 B lymphocytes, which is in part, due to higher levels of constitutively active Lyn. PMID: 19541367
    45. Findings confirm the importance of interaction of CRs CD21 and CD35 and CD19 for coreceptor activity in humoral immunity but identify a role for CRs in B-cell memory independent of CD19. PMID: 19706534
    46. CD21/35 promotes protective humoral immunity to S. pneumoniae and other strong TI-2 Ags through a complement-independent pathway by negatively regulating CD19 expression and PD-1 induction PMID: 19710450
    47. This molecule functions synergistically with the CD21 antigen to enhance signaling through the B cell antigen receptor in response to T cell-dependent, complement-tagged antigens. PMID: 11418645

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
  • 组织特异性：
    Detected on B cells in spleen, bone marrow, thymus and lymph nodes. Detected on peripheral blood lymphocytes (at protein level).
  • 数据库链接：

    KEGG: mmu:12478

    STRING: 10090.ENSMUSP00000032968

    UniGene: Mm.4360