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  4. Recombinant Mouse ATP-binding cassette sub-family G member 1 (Abcg1)

Recombinant Mouse ATP-binding cassette sub-family G member 1 (Abcg1)

  • 货号：
    CSB-CF714487MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Abcg1; Abc8; Wht1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-666
  • 氨基酸序列
    MACLMAAFSVGTAMNASSYSAAMTEPKSVCVSVDEVVSSNVDEVETDLLNGHLKKVDNNF TEAQRFSSLPRRAAVNIEFKDLSYSVPEGPWWKKKGYKTLLKGISGKFNSGELVAIMGPS GAGKSTLMNILAGYRETGMKGAVLINGMPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMV SAHLKLQEKDEGRREMVKEILTALGLLPCANTRTGSLSGGQRKRLAIALELVNNPPVMFF DEPTSGLDSASCFQVVSLMKGLAQGGRSIVCTIHQPSAKLFELFDQLYVLSQGQCVYRGK VSNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDADYKRDLGGD TDVNPFLWHRPAEEDSASMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLRITSHIG IGLLIGLLYLGIGNEAKKVLSNSGFLFFSMLFLMFAALMPTVLTFPLEMSVFLREHLNYW YSLKAYYLAKTMADVPFQIMFPVAYCSIVYWMTSQPSDAVRFVLFAALGTMTSLVAQSLG LLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFDTIPAYLQWMSYISYVRYGFEGVILS IYGLDREDLHCDIAETCHFQKSEAILRELDVENAKLYLDFIVLGIFFISLRLIAYFVLRY KIRAER
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.
  • 基因功能参考文献：
    1. HSP70 promotes the progression of atherosclerosis in apoE-/- mice by suppressing the expression of ABCA1 and ABCG1 through the JNK/Elk-1 pathway. PMID: 29678642
    2. TMP upregulated the protein stability of ABCA1 without affecting ABCG1. Accordingly, TMP regulated the expression of SR-A, CD36, ABCA1 and ABCG1 in aortas of ApoE-/- mice, which resembled the findings observed in macrophages. PMID: 28791414
    3. ABCG1 regulates pulmonary surfactant metabolism PMID: 28264879
    4. ABCG1 regulates T cell differentiation into Tregs, highlighting a pathway by which cholesterol accumulation can influence T cell homeostasis in atherosclerosis PMID: 27482882
    5. Our data indicate that a combination of vildagliptin and pravastatin significantly induces the expression of LXR-ABCA1/ABCG1 cascade and improves cholesterol efflux (P > 0.05) in adipocytes. Our data may explain, at least in part, the improvement in HDL-C levels observed in patients receiving both medications PMID: 27251372
    6. ABCG1 may play a protective role in early-stage atherosclerosis by reducing endothelial activation induced by oscillatory shear stress via suppressing the inflammatory response. PMID: 27297110
    7. Endothelial cholesterol efflux pathways mediated by ABCA1 and ABCG1 are nonredundant and atheroprotective, reflecting preservation of endothelial NO synthase activity and suppression of endothelial inflammation, especially in regions of disturbed arterial blood flow. PMID: 27199450
    8. ABCG1, irrespective of either a leucine or proline at position 550, is an intracellular protein that localizes to vesicles of the endosomal pathway where it functions to mobilize sterols away from the endoplasmic reticulum and out of the cell. PMID: 27230131
    9. our study suggests that MEK1/2 inhibitors activate macrophage ABCG1 expression/RCT, and inhibit foam cell formation and lesion development by multiple mechanisms, supporting the concept that ERK1/2 inhibition is anti-atherogenic PMID: 27365310
    10. miR-33 augments macrophage lipid rafts and enhances proinflammatory cytokine induction and NF-kappaB activation by LPS. This occurs through an ABCA1- and ABCG1-dependent mechanism and is reversible by interventions upon raft cholesterol and by ABC transporter-inducing liver X receptor agonists. PMID: 27471270
    11. ABCG1 expression was down-regulated by TLR4, which induces inflammation and lipid accumulation in vascular smooth muscle cells via PPARgamma/LXRalpha signaling. PMID: 27807703
    12. Visfatin upregulated CD36 and SRA expression and downregulated ABCA1 and ABCG1 expression, subsequently increased ox-LDL uptake and decreased cholesterol efflux, and finally promoted foam cell formation via the PI3K- and ERK-dependent pathways. PMID: 26536203
    13. Leu at position 550/562 in mABCG1/hABCG1 is critical for their plasma membrane localization but not for ABCG1-mediated cholesterol efflux. PMID: 26695502
    14. 3beta,5alpha,6beta-cholestanetriol and 25-hydroxycholesterol are physiologic substrates for ABCG1 PMID: 24833118
    15. Our data suggest that GLP-1-based therapy modulate ABCA1/ABCG1 expression in adipocytes potentially through an LXR-alpha mediated process. PMID: 26603933
    16. The absence of ABCG1 inhibits tumour growth through modulation of macrophage function within the tumour, and illustrates a link between cholesterol homeostasis and cancer. PMID: 25724068
    17. data support the impact of genes from the Abcg1-U2af1 region as modifiers of Tc1-dependent memory and locomotor phenotypes in Tc1 mouse model of Down syndrome. PMID: 25706610
    18. HDL enhances transendothelial cholesterol transport by activation of a mechanism involving ABCA1, ABCG1 and SR-B1 but not involving PI3K and Akt. PMID: 26255968
    19. AOPPs increase accumulation of lipids and exacerbate atherosclerosis through downregulation of ABCA1 and ABCG1 expression, and the JAK-LXRalpha signaling pathway in apoE-KO mice. PMID: 25262842
    20. The role of cellular cholesterol transport proteins including adenosine triphosphate binding cassette transporter A1 (ABCA1), G1 (ABCG1) and scavenger receptor class B type I (SR-BI) in diabetic nephropathy, was determined. PMID: 25181357
    21. The study identifies a major role of adipocyte ABCG1 in adiposity and fat mass growth and suggests that adipose ABCG1 might represent a potential therapeutic target in obesity. PMID: 25249572
    22. ABCG1 is involved in cellular vitamin E efflux. PMID: 25462452
    23. Results of this study show that the ABC transporter ABCA1 (but not ABCG1) plays a role in the early remodeling process that ensues brain injury PMID: 24661912
    24. Data indicate that transgenic S100/calgranulin has no direct effect on cholesterol efflux in macrophages, but rather promotes IL-22 secretion, which reduces cholesterol efflux in macrophages by decreasing the expression of ABC transporter ABCG1. PMID: 24367046
    25. The expression of ABCG1 is suppressed by activation of NF-kappaB. PMID: 24360166
    26. found that ABCA1 and ABCG1 were expressed in all retinal cell types, and that their expression was decreased in Hfe(-/-) retina PMID: 24462739
    27. Anti-inflammatory effects of LXR activators are of key importance to their antiatherosclerotic effects in Ldlr knockout mice independent of cholesterol efflux pathways mediated by macrophage ABCA1/G1. PMID: 24311381
    28. MiR-128-2 inhibits the expression of ABCA1, ABCG1 and RXRalpha directly through a miR-128-2-binding site within their respective 3'untranslated regions. PMID: 23990020
    29. BMP macrophage accumulation reduced cholesterol efflux to both apolipoprotein A1 and high-density lipoprotein by 40% and correlated with a 40% decrease in mRNA contents of ABCA1, ABCG1, and liver-X receptor alpha and beta. PMID: 23788762
    30. Macrophage deficiency of ABCA1/G1 is proatherogenic by promoting plaque inflammation and a novel positive feedback loop in which cholesterol-laden splenic macrophages signal to produce monocytes, with suppression by macrophage cholesterol efflux pathways. PMID: 23572498
    31. Mice with defects in cholesterol efflux pathways due to deficiencies of the ATP binding cassette transporter ABCG1 displayed a dramatic increase in HSPC mobilization and extramedullary hematopoiesis. PMID: 22862945
    32. RAW 264.7 macrophage ABCA1 and ABCG1 expression was repressed by unsaturated fatty acids. PMID: 22209005
    33. posttranslational control is absent from the murine ABCG1 homolog. PMID: 22872754
    34. Changes in intracellular cholesterol homeostasis regulated by ABCG1 profoundly impact iNKT cell development and function. PMID: 23100511
    35. pathways for cholesterol trafficking out of adipose tissue involve adipose tissue macrophage egress as well as ABCG1 mediated cholesterol efflux PMID: 22179025
    36. ABCG1 controls LPL activity and promotes lipid accumulation in human macrophages in the presence of triglyceride-rich lipoproteins. PMID: 22772754
    37. ABCG1 coordinates airway adaptive immunity, with its deletion suppressing adaptive lung eosinophilia through an IL-17-dependent mechanism. PMID: 22539789
    38. Data indicate that hepatic nascent HDL formation is highly dependent on ABCA1 but not on ABCG1 or SR-BI. PMID: 22190590
    39. ABCG1 abscence leads to increased lesions in early atherosclerotic Ldl receptor-deficient mice, while in more advanced stages of atherosclerosis enhanced apoptosis and/or compensatory mechanisms lead to retarded lesion progression. PMID: 22196936
    40. Data demonstrate that ABCG1 is an intracellular sterol transporter that localizes to endocytic vesicles. PMID: 22095132
    41. ABCA1 and ABCG1 each make complimentary and important contributions to beta-cell function by maintaining islet cholesterol homeostasis in vivo. PMID: 22315310
    42. Our results indicate that stigmasterol increases ABCA1 and ABCG1 expression. PMID: 21111593
    43. study provided evidence that the ubiquitin-proteasome system is involved in ABCA1/G1 degradation PMID: 21817095
    44. observations indicate that calpain promotes ABCG1 degradation by cleaving cell surface-resident ABCG1, and consequently reduces the expression and cholesterol efflux function of ABCG1 PMID: 21295304
    45. LXRalpha-dependent upregulation of ABCA1 and ABCG1 may mediate the beneficial effect of alpha-LA on foam cell formation. PMID: 21034810
    46. Our data indicate that inflammatory remodeling of HDL impacts ABCG1-dependent efflux independent of serum amyloid A. PMID: 21138980
    47. Deletion of ABCA1 and ABCG1 impairs macrophage migration because of increased Rac1 signaling. PMID: 21148432
    48. ABCG1 is expressed in cultured human keratinocytes and murine epidermis PMID: 20675829
    49. atheroprotective role of vascular ABCG1, especially in the aortic arch, likely related to its role in the preservation of endothelial NO synthase activity. PMID: 20705913
    50. The overexpression of GX sPLA(2) significantly reduced ABCA1 and ABCG1 expression in J774 macrophage-like cells, whereas GX sPLA(2) deficiency in peritoneal macrophages was associated with enhanced expression. PMID: 20844270

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell membrane.
  • 蛋白家族：
    ABC transporter superfamily, ABCG family, Eye pigment precursor importer (TC 3.A.1.204) subfamily
  • 组织特异性：
    Expressed mainly in brain, thymus, lung, adrenals, spleen and placenta. Little or no expression in liver, kidney, heart, muscle or testes.
  • 数据库链接：

    KEGG: mmu:11307

    STRING: 10090.ENSMUSP00000024829

    UniGene: Mm.15691