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  4. Recombinant Lactobacillus casei Thiamine transporter ThiT (thiT)

Recombinant Lactobacillus casei Thiamine transporter ThiT (thiT)

  • 中文名称：
    Recombinant Lactobacillus casei Thiamine transporter ThiT(thiT)
  • 货号：
    CSB-CF311819LAN
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    thiT
  • Uniprot No.：
  • 别名：
    thiT; LSEI_1757; Thiamine transporter ThiT; Thiamine ECF transporter S component ThiT
  • 种属：
    Lactobacillus casei (strain ATCC 334)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-201
  • 氨基酸序列
    MQQHKQLVVILETAIIAAFAMALTYIPHTTGVSAIELNYGLIPIAVLAMRRGLVPAAWAG FVWGILDLILRGIGGGSVLNPLQGILEYPIAFTLVGLMGLTFASFQKAVRGSEKVKASGY AFAGIIIGTFAKYFIHFIAGVVFWGAYAPKGTNVWVYSLIVNGGSALFSTVLTIVVVGVL LTVAPQLFVAKDGKSFSTKAA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Thiamine-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins and transport it into cells. Binds thiamine with a dissociation constant of 0.5 nM. Upon coexpression with its energy-coupling factor (ECF) ABC-transporter complex EcfA1A2T in Lactococcus lactis subsp. cremoris (strain NZ9000) allows thiamine uptake; uptake requires both ThiT and EcfA1A2T.
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family, Thiamine transporter subfamily
  • 数据库链接：

    KEGG: lca:LSEI_1757