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  4. Recombinant Human Urea transporter 1 (SLC14A1)

Recombinant Human Urea transporter 1 (SLC14A1)

  • 货号：
    CSB-CF615678HU(A4)
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    SLC14A1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    48.6 kDa
  • 表达区域：
    1-389aa
  • 氨基酸序列
    MEDSPTMVRVDSPTMVRGENQVSPCQGRRCFPKALGYVTGDMKELANQLKDKPVVLQFIDWILRGISQVVFVNNPVSGILILVGLLVQNPWWALTGWLGTVVSTLMALLLSQDRSLIASGLYGYNATLVGVLMAVFSDKGDYFWWLLLPVCAMSMTCPIFSSALNSMLSKWDLPVFTLPFNMALSMYLSATGHYNPFFPAKLVIPITTAPNISWSDLSALELLKSIPVGVGQIYGCDNPWTGGIFLGAILLSSPLMCLHAAIGSLLGIAAGLSLSAPFEDIYFGLWGFNSSLACIAMGGMFMALTWQTHLLALGCALFTAYLGVGMANFMAEVGLPACTWPFCLATLLFLIMTTKNSNIYKMPLSKVTYPEENRIFYLQAKKRMVESPL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.
  • 基因功能参考文献：
    1. The urea transporter subtypes, UT-A1 and UT-B1, were expressed in the skin basal cell layer and exocrine sweat glands. The abundance of UT-A1 and UT-B1 in uremic sweat glands was significantly increased in UP, while the expression of AQP5 was decreased. PMID: 29279852
    2. In addition to the well known Polynesian Jknull allele, three Jknull alleles were detected including one novel Jknull allele: JKA (130A, 220G). PMID: 26969102
    3. The Jk(a-b-) phenotype in the Chinese population shows several different molecular mechanisms. A novel missense mutation nt737T>G of JK gene was found as associated with Jk(a-b-) phenotype. PMID: 25807964
    4. Data indicate that urea transporter UT-A1 successfully trafficking to the apical membrane of the epithelial cells is crucial for the regulation of urea transport. PMID: 23698785
    5. Results suggested that polymorphism in TERTC/T and SLC14A1C/T confirmed high risk for BC in North Indian population. PMID: 25218484
    6. Reduction or loss of UT-B expression may be related to the incidence, progression and invasiveness of bladder urothelial carcinoma. PMID: 25445116
    7. Studies indicate that expression of urea transporter UT-B confers high urea permeability to erythrocytes. PMID: 25298342
    8. Studies indicate that acid substitution in the urea transporter Slc14A1 UT-B protein determines the erythrocyte Kidd blood group antigen. PMID: 25298346
    9. UT-B should be considered as a new member of the water channel family. PMID: 24376529
    10. High-throughput Kell, Kidd, and Duffy matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry-based blood group genotyping of 4000 donors shows close to full concordance with serotyping and detects new alleles. PMID: 24845979
    11. these data confirm the presence of UT-B protein within the human bladder. PMID: 25209859
    12. Novel polymorphisms in exon 9 of the JSLC14A1 gene in Japanese individuals associated with the Jk(a-b-) phenotype. PMID: 24877238
    13. Thienoquinoline PU-14 is a selective UT inhibitor and has urea-selective diuretic activity. PMID: 23486518
    14. SLC14A1 could be a unique urea transporter in the bladder that has the ability to influence urine concentration and that this mechanism might explain the increased bladder cancer susceptibility associated with rs10775480. PMID: 23754249
    15. Inhibitor and mutagenesis studies and results of molecular dynamics simulations suggest that NH and HO pass through the three monomeric urea channels in UT-B. PMID: 23552862
    16. Four novel JK-null alleles were noted to be associated with the Jk(a-b-) phenotype. PMID: 22738189
    17. the rate of urea conduction in UT-B is increased by hypoosmotic stress, and the site of osmoregulation coincides with the location of the energy barrier PMID: 22733730
    18. Loss of SLC14A1 is associated with lung adenocarcinoma. PMID: 22223368
    19. rs17674580, or other sequence variants of SLC14A1, indirectly modifies urinary bladder cancer risk by affecting urine production. PMID: 21750109
    20. Genetic variation in SLC14A1 could provide new etiological insights into bladder carcinogenesis. PMID: 21824976
    21. A new allele JK*01W has 3 changes (130G>A, 588A>G, Intron 9-46a>g)in addition to 838G. It is associated with weak expression of the Jk-a antigen on erythrocytes. PMID: 21309779
    22. Results illustrate differential protein abundance of functional UT-B protein in different sections of the human colon, strongly correlating to regions that contain the largest populations of intestinal bacteria. PMID: 19926813
    23. gene encodes the Kidd blood group antigens PMID: 12093813
    24. Kidd antigen/UT-B urea transporter is physiologically expressed in the human colon epithelium, where it could participate in the transport of urea across the colon mucosa. PMID: 14985236
    25. high frequency of JK null allele in Taiwanese indigenous groups PMID: 18713105
    26. Three blood group antigens genes, namely CD55, CD151, and SLC14A1, have been subjected to balancing selection, a process, rare outside MHC genes, which maintains variability at a locus. PMID: 18997004

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Urea transporter family
  • 组织特异性：
    Detected in erythrocytes (at protein level). Erythrocytes.
  • 数据库链接：

    HGNC: 10918

    OMIM: 111000

    KEGG: hsa:6563

    STRING: 9606.ENSP00000390637

    UniGene: Hs.101307