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  4. Recombinant Human Potassium channel subfamily K member 9 (KCNK9)

Recombinant Human Potassium channel subfamily K member 9 (KCNK9)

  • 货号：
    CSB-CF889068HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    KCNK9
  • Uniprot No.：
  • 别名：
    (Acid-sensitive potassium channel protein TASK-3)(TWIK-related acid-sensitive K(+) channel 3)(Two pore potassium channel KT3.2)(Two pore K(+) channel KT3.2)
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    48.3 kDa
  • 表达区域：
    1-374aa
  • 氨基酸序列
    MKRQNVRTLSLIVCTFTYLLVGAAVFDALESDHEMREEEKLKAEEIRIKGKYNISSEDYRQLELVILQSEPHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTDAGKAFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLLKRIKKCCGMRNTDVSMENMVTVGFFSCMGTLCIGAAAFSQCEEWSFFHAYYYCFITLTTIGFGDYVALQTKGALQKKPLYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNSEDERRDAEERASLAGNRNSMVIHIPEEPRPSRPRYKADVPDLQSVCSCTCYRSQDYGGRSVAPQNSFSAKLAPHYFHSISYKIEEISPSTLKNSLFPSPISSISPGLHSFTDHQRLMKRRKSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    pH-dependent, voltage-insensitive, background potassium channel protein.
  • 基因功能参考文献：
    1. observations implicate TASK-3 as a critical factor in cell cycle progression and corroborate its potential as a therapeutic target in breast cancer treatment. PMID: 29596383
    2. point mutations in DLK1 and KCNK9 at least do not seem to be a common cause of central precocious puberty in girls. PMID: 28672280
    3. In conclusion, we have identified the first selective activator of the two-pore domain potassium channel TASK3. PMID: 28882594
    4. KCNK9 imprinting syndrome is a rare cause of intellectual disability, congenital hypotonia, palatal abnormalities, and occasional seizures. PMID: 27151206
    5. Our results provide evidence for new loci influencing abdominal visceral (BBS9, ADCY8, KCNK9) and subcutaneous (MLLT10/DNAJC1/EBLN1) fat, and confirmed a locus (THNSL2) previously reported to be associated with abdominal fat in women. PMID: 26480920
    6. TASK-1 and TASK-3 may form heterodimers in human atrial cardiomyocytes. PMID: 25655935
    7. During conductance simulation experiments, both TASK-3 and TREK-1 channels were able to repolarise the membrane once AP threshold was reached PMID: 25482670
    8. The findings of this study suggest that variations in KCNK9 genes are associated with both mild and severe persistent breast pain after breast cancer surgery. PMID: 25599232
    9. Diacylglycerol mediates regulation of TASK1 and TASK3 potassium channels by GNAQ. PMID: 25420509
    10. Interference with TASK-3 channel expression, therefore, induces caspase-dependent and -independent apoptosis of melanoma cells, most likely via causing mitochondrial depolarization. PMID: 25318378
    11. K2P3.1 and K2P9.1 undergo rapid dynamin-dependent endocytosis PMID: 23807092
    12. Mutations of KCNK9 or epigenetic disturbances within the PEG13 imprinted cluster do not significantly contribute to the cause of the developmental disabilities tested in this study. PMID: 24980697
    13. Two SNPs replicated: the paternal rs2471083-C allele (located near the imprinted KCNK9 gene) and the paternal rs3091869-T allele (located near the SLC2A10 gene) increased BMI equally (beta = 0.11 (SD), P<0.0027) PMID: 25078964
    14. For KCNK9, two SNPs (i.e., rs3780039, rs11166921) were associated with the occurrence of preoperative breast pain. PMID: 24392765
    15. We observed co-localization of the TASK-3 protein and a mitochondrial marker in the mitochondria of HaCaT cells. PMID: 24126847
    16. By both a gain-of-function additional mutation of TASK3 channels. PMID: 24342771
    17. TNFalpha activation and TASK3 channel activity can promote cellular apoptosis. PMID: 24307172
    18. Overexpression of TASK-3 expression is associated with ovarian cancer. PMID: 23564779
    19. Results of an exploratory study suggest that blood pressure and aldosterone production may be affected by variations in KCNK9. The findings could have relevance to risk for hypertension. PMID: 22893713
    20. an increase in TASK-3 expression levels reduces cell migration/invasion in breast cancer cells PMID: 21910834
    21. Depletion of the endogenous phosphatidylinositol-4,5-bisphosphate pool in living cells consistently finds no change in currents mediated by TASK-1 and TASK-3. PMID: 21540350
    22. TASK-3 immunoreactivity was detected in both astrocytes and microglia of temporal lobe epilepsy patients. PMID: 21710317
    23. TASK-3 channels are functionally present in the mitochondria of the melanoma cells, and their function is essential for the survival of these cells. PMID: 21512417
    24. cAMP-dependent protein kinase is responsible for the phosphorylation of the terminal serine in both K(2P)3.1 and K(2P)9.1 PMID: 21357689
    25. Western analysis confirms expression of TASK1 and TASK3 in medulloblastoma cells. PMID: 20931182
    26. KCNK9 gene is imprinted in human and mouse, exhibiting preferential expression from the maternal allele in brain. PMID: 17704508
    27. TASK-1 and 3 are determinant of aldosterone secretion and adrenocortical zonation. PMID: 20049674
    28. TASK-3 responds to voltage in a way that reveals gating at its inner, cytoplasmic mouth through movements of membrane helices M2 and M4. PMID: 19703964
    29. TASK-1 and TASK-3 differed insofar as a large portion of the C terminus was necessary for the full effects of halothane and TRH on TASK-3 but not on TASK-1 PMID: 11886861
    30. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation. PMID: 12676587
    31. results establish a direct link between the potassium channel activity of TASK3 and its oncogenic functions and imply that blockers for this potassium channel may have therapeutic potential for the treatment of cancers PMID: 12782791
    32. TASK3 is expressed in pia mater, astrocytes, Purkinje and granule cells PMID: 15197476
    33. TASK-3 channels underlie a major component of cerebellar granule neurons leak current which is sensitive to block by zinc PMID: 15284350
    34. Overexpression rather than mutation of the KCNK9 gene may contribute to the development of colorectal cancers. PMID: 15601307
    35. The expression pattern of TASK-3 in Melanoma cells is determined. PMID: 17013562
    36. (Glutamic acid-aspartic acid-glutamic acid) in human TASK-3 is a major determinant of the rate of endoplasmic reticulum export and is required for efficient surface expression of the channel. PMID: 17547699
    37. TASK-3 channels are present in the mitochondria in both malignantly transformed and healthy cells, suggesting that they might have roles in ensuring mitochondrial functions. PMID: 18094996
    38. TASK3 channels may possibly represent a novel molecular target for the treatment of human glioblastoma PMID: 18217213
    39. K(2)P channels as novel potassium conductance on T lymphocytes critically influencing T cell effector function and identify a possible molecular target for immunomodulation in T cell-mediated autoimmune disorders PMID: 18375952
    40. the TASK3 M1P1 loop lies close to the pore, regulating TASK3 channel activity PMID: 18417474
    41. a mutation in the genomically imprinted potassium channel KCNK9 may have a role in the maternally inherited Birk Barel mental retardation dysmorphism syndrome PMID: 18678320
    42. Thus, regulated expression of TASK channels might contribute to a molecular switch between death and survival of neurons in autoimmune CNS inflammation. PMID: 18824070
    43. Both TASK-3 and TREK-1 are functionally operational in the adrenocortical H295R cell line, modulate membrane potential and aldosterone secretion. PMID: 18854423

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  • 相关疾病：
    Birk-Barel mental retardation dysmorphism syndrome (BIBAS)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Two pore domain potassium channel (TC 1.A.1.8) family
  • 组织特异性：
    Mainly found in the cerebellum. Also found in adrenal gland, kidney and lung.
  • 数据库链接：

    HGNC: 6283

    OMIM: 605874

    KEGG: hsa:51305

    STRING: 9606.ENSP00000302166

    UniGene: Hs.493037