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  4. Recombinant Human Neuronal acetylcholine receptor subunit beta-4 (CHRNB4)

Recombinant Human Neuronal acetylcholine receptor subunit beta-4 (CHRNB4)

  • 中文名称：
    Recombinant Human Neuronal acetylcholine receptor subunit beta-4(CHRNB4)
  • 货号：
    CSB-CF005398HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    CHRNB4
  • Uniprot No.：
  • 别名：
    CHRNB4; Neuronal acetylcholine receptor subunit beta-4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-498
  • 氨基酸序列
    RVANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWL KQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSV LWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMDDFTPSGEW DIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLTTLLAILVFYLPSDCG EKMTLCISVLLALTFFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHH RSPSTHTMAPWVKRCFLHKLPTFLFMKRPGPDSSPARAFPPSKSCVTKPEATATSTSPSN FYGNSMYFVNPASAASKSPAGSTPVAIPRDFWLRSSGRFRQDVQEALEGVSFIAQHMKND DEDQSVVEDWKYVAMVVDRLFLWVFMFVCVLGTVGLFLPPLFQTHAASEGPYAAQRD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 基因功能参考文献：
    1. Identified a novel exon variant Ser140Gly of CHRNB4 , and validated two previously reported variants Tyr215Tyr and Asp398Asn are significantly associated with COPD risk in Chinese. PMID: 25891420
    2. We identified 10 low frequency non-synonymous variants in CHRNB4, and found a highly significant correlation between carrier status weighted by either ACh EC50 or by low response to nicotine. PMID: 24804708
    3. Unmethylation of the CHRNB4 gene is an unfavorable prognostic factor in non-small cell lung cancer. PMID: 25172267
    4. Elucidation of molecular impediments in the alpha6 subunit for in vitro expression of functional alpha6beta4* nicotinic acetylcholine receptors. PMID: 24085295
    5. the importance of SNP rs1948 on the regulation of CHRNB4 expression PMID: 23691088
    6. epigenetic deregulation of nicotinic acetylcholine receptor subunit (nAChR) genes which in the case of CHRNB4 is strongly associated with genetic lung cancer susceptibility variants and a functional impact on tumorigenic potential PMID: 22945651
    7. Genetic variation in the CHRNA5-CHRNA3-CHRNB4 gene cluster, encoding the alpha5, alpha3, and beta4 nAChR subunits, respectively, has been shown to increase vulnerability to tobacco dependence PMID: 23143843
    8. The SNP rs 12914008 on CHRNB4 that is not statically associated with COPD risk is a positive factor in Bupropion treatment while its effect on NRT is controversial. PMID: 23061658
    9. Overexpression of alpha3/alpha5/beta4 nicotinic acetylcholine receptor subunits produces changes in two specific aspects of executive functioning--working memory and response inhibition--that may be related to nicotine dependence vulnerability. PMID: 22024278
    10. functional properties of nAChRs containing beta4R349C subunit; mutation caused reduction in potency of ACh and nicotine, decreased density of whole-cell current evoked by maximal transmitter concentrations, altered kinetics of ACh-evoked whole-cell currents PMID: 21107856
    11. Single nucleotide polymorphisma in CHRNB4 showed suggestive association with the comorbidity of depression and nicotine dependence. PMID: 22241830
    12. Compared with etomidate, carboetomidate's higher hydrophobicity is associated with greater inhibition of alpha4/beta2 neuronal nicotinic acetylcholine receptors. PMID: 22543065
    13. Missense variants at conserved residues in CHRNB4 are associated with lower risk for nicotine dependence in African Americans and European Americans. PMID: 22042774
    14. Quantitative trait disequilibrium test (QTDT) significant association was detected between age at onset of daily smoking and variants located upstream of CHRNB4. PMID: 22438940
    15. Single nucleotide polymorphism (SNP) CHRNB4 on chromosome 15 is not associated with Parkinson's disease risk in the overall anaylsis or after stratifying on smoking status. PMID: 21228559
    16. alpha6beta4* nAChRs are expressed and contribute to exocytosis in human chromaffin cells of the adrenal gland, the main source of adrenaline under stressful situations. PMID: 21917987
    17. Single Nucleotide Polymorphisms in CHRNB4 is associated with smoking persistence in African Americans. PMID: 21436384
    18. Variation in the nicotinic acetylcholine receptor gene cluster CHRNA5-CHRNA3-CHRNB4 influences cognitive flexibility differently in African Americans and European Americans. PMID: 20631687
    19. CHRNB4 subunit is expressed in the soma of the majority of pyramidal cells, with fairly consistent immunoreactivity observed throughout the different regions of the hippocampus. PMID: 12663058
    20. The receptors containing the R136W and M467V mutations (or variants) had a higher sensitivity to acetylcholine and lower EC50 than the wild-type. PMID: 15742216
    21. CHRNB4 seem to exert no relevant influence on smoking cessation probability in heavy smokers in the general population. PMID: 18996504

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Beta-4/CHRNB4 sub-subfamily
  • 数据库链接：

    HGNC: 1964

    OMIM: 118509

    KEGG: hsa:1143

    STRING: 9606.ENSP00000261751

    UniGene: Hs.624178