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  4. Recombinant Human Cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6)

Recombinant Human Cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6)

  • 货号：
    CSB-CF002277HU(A4)
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    ATF6; Cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; ATF6-alpha
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-670
  • 氨基酸序列
    MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQLEAANETYENNFDNLDFDLDLMPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSSPRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSAEPLKEDKPVTGPRNKTENGLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIISLQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSPVNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKAALSENEQLKKENGTLKRQLDEVVSENQRLKVPSPKRRVVCVMIVLAFIILNYGPMSMLEQDSRRMNPSVSPANQRRHLLGFSAKEAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEPLLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQGSNSQLMAVQYTETTSSISRNSGSELQVYYASPRSYQDFFEAIRRRGDTFYVVSFRRDHLLLPATTHNKTTRPKMSIVLPAININENVINGQDYEVMMQIDCQVMDTRILHIKSSSVPPYLRDQQRNQTNTFFGSPPAATEATHVVSTIPESLQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane. Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR).; Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
  • 基因功能参考文献：
    1. 147 is a pro-drug that preferentially activates ATF6 signaling through a mechanism involving localized metabolic activation and selective covalent modification of endoplasmic reticulumresident proteins that regulate ATF6 activity. PMID: 30084354
    2. sustained intestinal activation of ATF6 in the colon to promote dysbiosis and microbiota-dependent tumorigenesis. PMID: 30063920
    3. results support a critical role of ATF6alpha in the establishment and maintenance of cellular senescence in normal human fibroblasts via the up-regulation of a COX2/PGE2 intracrine pathway. PMID: 28803844
    4. The results demonstrated that high expression of activated ATF6 aggravates ER stressinduced VEC apoptosis through the mitochondrial apoptotic pathway. Furthermore, in response to ER stress, ATF6 upregulates the expression of caspase3, caspase9, CHOP, cytochrome c and Bax/Bcl2. PMID: 29512699
    5. Reporters constructed to monitor each mechanism show that phenobarbital-induced endoplasmic reticulum membrane expansion depends on transmembrane domain-induced ATF6 PMID: 30086303
    6. ATF6 plays a distinct role in viral protein stability and the host uses different cleavage strategies, rather than conventional cleavage by generating p50ATF6, to combat viral infection. PMID: 29386036
    7. Three branches of the Unfolded Protein Response (UPR) have been described, including the activation of the inositol-requiring enzyme 1 (IRE1), the pancreatic ER kinase (PKR)-like ER kinase (PERK), and the activating transcription factor 6 (ATF6). PMID: 28105371
    8. The expression of ASNS was significantly elevated when ATF6 was over expressed. The expressions of these 2 genes were both decreased in hepatocellular carcinoma (HCC)patients, and it was more significantly with ASNS. The mRNA levels of ASNS and ATF6 were positively correlated with each other. rs34050735 was associated with HCC in the case-control study and also an independent predictor of overall survival of HCC patients. PMID: 28629319
    9. Human ATF6 mutations interrupt distinct sequential steps of the ATF6 activation mechanism. PMID: 28028229
    10. Findings indicate a central role for activating transcription factor 6 (ATF6alpha) in the establishment of morphological features of senescence in normal primary fibroblasts. PMID: 27563820
    11. A novel homozygous c.1691A>G (p.(Asp564Gly)) ATF6 mutation was identified in two siblings with autosomal recessive cone-rod dystrophy. PMID: 28812650
    12. This review shown that the achromatopsia arising from genetic mutations in Activating Transcription Factor 6 (ATF6). PMID: 27117871
    13. In summary, our study revealed a negative regulation of the UPR transducer ATF6 through post-translational SUMOylation. The information from this study will not only increase our understanding of the fine-tuning regulation of the UPR signaling but will also be informative to the modulation of the UPR for therapeutic benefits. PMID: 29061306
    14. Low ATF6 expression is associated with cancer. PMID: 28157699
    15. Activating transcription factor 6 (ATF6alpha) pathway and ER-associated protein degradation (ERAD) are elevated in salivary glands of Sjogren's syndrome patients (SS). PMID: 27461470
    16. A transcription factor complex consisting of ATF6 (an endoplasmic reticulum-resident factor) and C/EBP-beta is required for the IFN-gamma-induced expression of DAPK1 IFN-gamma-induced proteolytic processing of ATF6 and phosphorylation of C/EBP-beta are obligatory for the formation of this transcriptional complex PMID: 27590344
    17. s observed the cleavage of ATF6, the phosphorylation of MRLC, and the expression of death-associated protein kinase (DAPK1) by western blotting; the transcription of DAPK1 by RT-PCR; and the subcellular localization of ATF6 and mAtg9 by immunofluorescence. PMID: 27085326
    18. Genetic Variations in ATF6 (rs2070150) is not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection. PMID: 26925648
    19. We found that HCS identified compounds which inhibited ATF6 nuclear translocation with high specificity, as confirmed by the luciferase reporter assay and western blot analysis PMID: 26707144
    20. results identify a role for DREAM silencing in the activation of ATF6 signaling, which promotes early neuroprotection in HD. PMID: 26752648
    21. Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy. PMID: 26261584
    22. These results confirm that HIV infection activates stress-response components and that antiretroviral therapy contributes to changes in the unfolded protein response activation profile. PMID: 25976933
    23. Defective podocyte insulin signaling through p85-XBP1 promotes ATF6-dependent maladaptive ER-stress response in diabetic nephropathy. PMID: 25754093
    24. protein expression were significantly higher in the placentas of women with early and late SPE than in the control women, whereas there were no differences in ATF6 and Ire1 mRNA and protein. PMID: 25675914
    25. autosomal recessive achromatopsia is caused by a frameshift mutation in ATF6 in this Pakistani family PMID: 26063662
    26. A crucial and unexpected role for ATF6A in human foveal development and cone function. PMID: 26029869
    27. ATF6 preferentially reduces the secretion and extracellular aggregation of destabilized, aggregation-prone variants of the amyloidogenic protein TTR, as compared to stable WT TTR and nonamyloidogenic TTR variants. PMID: 25444553
    28. The genetic variation in ATF6 is associated with pre-diabetes and has interactive effects with BMI on pre-diabetes in the Chinese Han population. PMID: 25302688
    29. Data show that silver nanoparticles induce activating transcription factor-6 (ATF-6) degradation, leading to activation of the NLRP-3 inflammasome and pyroptosis. PMID: 25593314
    30. our data demonstrate that CiC expression is activated during ER stress through the binding of ATF6alpha and XBP1 to an UPRE element located in the proximal promoter of Cic gene. PMID: 25450523
    31. Data shows that mutation of putative p38 MAPK phosphorylation sites in ATF6 suppresses its transcriptional induction of DAPK1. PMID: 25135476
    32. activating transcription factor 6 single polymorphism has a role in increased body mass index after kidney transplantation PMID: 22099811
    33. Activation of the ATF6 pathways of the UPR is sustained along with PERK signaling in melanoma cells subjected to pharmacological ER stress, and that this plays an important role in protecting melanoma cells from ER stressinduced apoptosis. PMID: 24240056
    34. ATF6 binds to the promoter of XBP1 and enhances the XBP1S expression in OA cartilage. PMID: 24269637
    35. activation of the ATF6 pathway of the UPR limits ATZ-dependent cell toxicity by selectively promoting ER-associated degradation of ATZ PMID: 21976666
    36. These studies indicate that activation of either IRE1, ATF6, or PERK prevents mutant rhodopsin from accumulating in the cells. PMID: 24664756
    37. Tick-borne encephalitis virus infection activates the IRE1 pathway and triggers expression of cleaved transcription factor 6 (ATF6) which suggest activation of ATF6 pathway. PMID: 24177270
    38. This study showed that the two compounds, polyP and isoquercitrin, have a co-enhancing effect on bone mineral formation and in turn might be of potential therapeutic value for prevention/treatment of osteoporosis. PMID: 24726443
    39. ATF6 mRNA expression was significantly decreased as the disease progressed PMID: 24302549
    40. these results indicate that ATF6alpha can regulate de novo cholesterol synthesis through stimulation of cholesterogenic gene expression. PMID: 23924739
    41. Mediator subunit MED25 plays a critical role in this process and identify a MED25 domain that serves as a docking site on Mediator for the ATF6alpha transcription activation domain. PMID: 23864652
    42. ATF6 represents a novel type of ERAD-Lm substrate requiring SEL1L for degradation despite its transmembrane nature. PMID: 24043630
    43. results demonstrate that ER stress-related proteins, particularly ATF6 and its downstream molecule CHOP, are involved in ox-LDL-induced cholesterol accumulation and apoptosis in macrophages PMID: 23037953
    44. ATF6 activation is significantly higher for the CASPR2-D1129H compared with the wild-type protein. PMID: 22872700
    45. one of three endoplasmic reticulum transmembrane protein sensors that signals the unfolded protein response [review] PMID: 22917505
    46. Data show that transfection of ORMDL3 in bronchial epithelial cells induced expression of MMP-9, ADAM-8, CCL-20, IL-8, CXCL-10, CXCL-11, oligoadenylate synthetases (OAS) genes, and selectively activated activating transcription factor 6 (ATF6). PMID: 23011799
    47. This study indicates that selectively activating ATF6 or PERK prevents mutant rhodopsin from accumulating in retinal cells. PMID: 22956602
    48. ATF6alpha functions at least in part by recruiting to the endoplasmic reticulum stress response enhancer elements of ER stress response genes a collection of RNA polymerase II coregulatory complexes. PMID: 22577136
    49. Increased expression of Endoplasmic Reticulum (ER) stress markers, GRP78, ATF6 and CHOP, in endometrioid endometrial carcinomas suggests a role for ER stress in endometrial cancer. PMID: 22146569
    50. BMP2 induces osteoblast differentiation through Runx2-dependent ATF6 expression, which directly regulates Oc transcription. PMID: 22102412

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  • 相关疾病：
    Achromatopsia 7 (ACHM7)
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein.; [Processed cyclic AMP-dependent transcription factor ATF-6 alpha]: Nucleus.
  • 蛋白家族：
    BZIP family, ATF subfamily
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 791

    OMIM: 605537

    KEGG: hsa:22926

    STRING: 9606.ENSP00000356919

    UniGene: Hs.492740