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  4. Recombinant Arabidopsis thaliana Phosphate transporter PHO1 (PHO1)

Recombinant Arabidopsis thaliana Phosphate transporter PHO1 (PHO1)

  • 中文名称：
    拟南芥PHO1重组蛋白
  • 货号：
    CSB-CF844594DOA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    PHO1
  • Uniprot No.：
  • 别名：
    PHO1; At3g23430; MLM24.26; Phosphate transporter PHO1; Protein PHO1; AtPHO1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-782
  • 氨基酸序列
    MVKFSKELEAQLIPEWKEAFVNYCLLKKQIKKIKTSRKPKPASHYPIGHHSDFGRSLFDP VRKLARTFSDKLFSNSEKPEILQVRRRRGSSETGDDVDEIYQTELVQLFSEEDEVKVFFA RLDEELNKVNQFHKPKETEFLERGEILKKQLETLAELKQILSDRKKRNLSGSNSHRSFSS SVRNSDFSAGSPGELSEIQSETSRTDEIIEALERNGVSFINSATRSKTKGGKPKMSLRVD IPDAVAGAEGGIARSIATAMSVLWEELVNNPRSDFTNWKNIQSAEKKIRSAFVELYRGLG LLKTYSSLNMIAFTKIMKKFDKVAGQNASSTYLKVVKRSQFISSDKVVRLMDEVESIFTK HFANNDRKKAMKFLKPHQTKDSHMVTFFVGLFTGCFISLFVIYIILAHLSGIFTSSDQVS YLETVYPVFSVFALLSLHMFMYGCNLYMWKNTRINYTFIFEFAPNTALRYRDAFLMGTTF MTSVVAAMVIHLILRASGFSASQVDTIPGILLLIFICVLICPFNTFYRPTRFCFIRILRK IVCSPFYKVLMVDFFMGDQLTSQIPLLRHLETTGCYFLAQSFKTHEYNTCKNGRYYREFA YLISFLPYFWRAMQCVRRWWDESNPDHLINMGKYVSAMVAAGVRITYARENNDLWLTMVL VSSVVATIYQLYWDFVKDWGLLNPKSKNPWLRDNLVLRNKNFYYLSIALNLVLRVAWIET IMRFRVSPVQSHLLDFFLASLEVIRRGHWNFYRVENEHLNNVGQFRAVKTVPLPFLDRDS DG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inositol polyphosphate sensor that associates with transcription factors to regulate inorganic phosphate (Pi) starvation responses. Probably acts by binding inositol polyphosphate via its SPX domain. Acts as a Pi exporter, mediating efflux of Pi out of cells. Transfers Pi from the epidermal and cortical cells to the root xylem vessels. Involved in the transfer of Pi from roots to shoots. Involved in abscisic acid (ABA) induction of stomatal closure and ABA repression of stomatal opening.
  • 基因功能参考文献：
    1. The Arabidopsis Pi exporters PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between the seed coat and embryo were analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1, or the pho1 phoh1 double mutant. PMID: 28943092
    2. Genome-wide association mapping identified PHOSPHATE 1 (PHO1) and genes such as Root System Architecture 1 (RSA1) associated with differences in root allometry, a highly plastic trait capturing the distribution of lateral roots along the primary axis. PMID: 23980140
    3. An important role of the EXS domain of PHO1 in a root-to-shoot signaling pathway linking phosphate deficiency response PMID: 26546667
    4. AtWRKY42 modulated Pi homeostasis by regulating the expression of PHO1 and PHT1;1 to adapt to environmental changes in inorganic phosphate (Pi) availability. PMID: 25733771
    5. PHR1 and PHO1 participate in the coregulation of Zn and Pi homeostasis. PMID: 24420568
    6. Expression of PHO1 in guard cells of Arabidopsis thaliana is required for full stomatal responses to abscisic acid. PMID: 22612335
    7. PHO1 can trigger Pi export in ectopic plant cells, strongly indicating that PHO1 is itself a Pi exporter. PMID: 22449068
    8. PHO1 over-expression in the shoot of Arabidopsis thaliana led to a two- to threefold increase in shoot inorganic phosphate content and a severe reduction in shoot growth. PMID: 21309867
    9. Reduction of PHO1 expression leads to wild-type growthof plants in phosphate-deficient media, despite Pi deficiency. PMID: 21288266
    10. Our results demonstrate that WRKY6 and WRKY42 are involved in Arabidopsis responses to low Pi stress by regulation of PHO1 expression. PMID: 19934380

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  • 亚细胞定位：
    Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    SYG1 (TC 2.A.94) family
  • 组织特异性：
    Predominantly in roots, but also weak expression in the lower part of the hypocotyl. In the stellar cells, including the pericycle and xylem parenchyma cells, but not in the cortical or epidermal cells. Expressed in guard cells.
  • 数据库链接：

    KEGG: ath:AT3G23430

    STRING: 3702.AT3G23430.1

    UniGene: At.23989