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  4. Recombinant Xenopus laevis RAC-alpha serine/threonine-protein kinase (akt1)

Recombinant Xenopus laevis RAC-alpha serine/threonine-protein kinase (akt1)

  • 货号：
    CSB-YP857839XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP857839XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP857839XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP857839XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP857839XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    akt1; RAC-alpha serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase Akt-1; xAkt; Protein kinase B; alpha; PKB alpha; RAC-PK-alpha
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-481
  • 氨基酸序列
    MNEVAIVKEG WLHKRGEYIK TWRPRYFLLK SDGTFIGYKE RPQDVDQLET PLNNFSVAKC QLMKTERPKP NTFIIRCLQW TTVIERTFHV DSPEEREEWI QVIQHVADNL KKQEEEMMEV RSGDSPSDNS GAEEMEVSHS KPKHKVTMNE FEYLKLLGKG TFGKVILVKE KATGRYYAMK ILKKEVIVAK DEVAHTLTEN RVLQNSRHPF LTALKYSFQT HDRLCFVMEY ANGGELFFHL SRERIFSEDR ARFYGAEIVS ALDYLHSEKN VVYRDLKLEN LMLDKDGHIK ITDFGLCKEG IKDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHEKLFE LILMEEIRFP RTLLPEAKSL LSGLLKKDPK QRLGGGPDDA KEIMQHKFFA GIVWQDVYEK KLVPPFKPQV TSETDTRYFD EEFTAQMITI TPPDQDDNFE FVDNERRPHF PQFSYSASGN A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    AKT1 is one of several closely related serine/threonine-protein kinases known as the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. Required for insulin-stimulated meiotic reinitiation during oocyte maturation.
  • 基因功能参考文献：
    1. These findings highlight novel and essential roles of PFKFB4 activity in later stages of neural crest (NC) development that are wired into the NC gene regulatory network. PMID: 29038306
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
  • 组织特异性：
    Expressed in the oocyte.
  • 数据库链接：

    KEGG: xla:399170

    UniGene: Xl.738