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  4. Recombinant Xenopus laevis Nodal homolog (nodal)

Recombinant Xenopus laevis Nodal homolog (nodal)

  • 货号：
    CSB-YP820937XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP820937XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP820937XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP820937XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP820937XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    nodal; nr1; Nodal homolog; Nodal-related protein 1; Xnr-1; Xnr1; nr-1
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    282-406
  • 氨基酸序列
    NRNENHHLS IGSIPSRHVE NGKPLCRRVD MIVDFEDIGW SSWIVYPKKY NAYRCEGACP IPLNETFKPT NHAYMKSVVK LYQPERVECP LCVPVKMSPL SMLYYEGDEV VLRHHQEMIV EECGCS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cooperation and regulatory loops of multiple nodals are essential for mesendoderm patterning in early embryos. Essential for mesoderm formation and axial patterning during embryonic development. Activates the activin-like signaling pathway to induce dorsal and ventral mesoderm in animal cap ectoderm. In addition, also dorsalizes ventral marginal zone (VMZ) tissues during gastrulation. Acts in a downstream signaling cascade via cripto and cer1 to mediate cardiogenesis in embryonic mesoderm. Directs the orientation of the left-right axis by driving the left-specific gene cascade in the left lateral plate mesoderm.
  • 基因功能参考文献：
    1. FoxH1 and Grg4 occupy the Xnr1 enhancer, and Grg4 occupancy is dependent on the FoxH1 EH1 motif. Grg4 occupancy at the Xnr1 enhancer significantly decreases with Nodal activation or Smad2 overexpression, while FoxH1 occupancy is unaffected. These results suggest that Nodal-activated Smad2 physically displaces Grg4 from FoxH1, an essential feature of the transcriptional switch mechanism. PMID: 27085753
    2. Data suggest that Coco acts as a critical target of flow, suggesting that symmetry is broken by flow-mediated left-asymmetric release of Nodal repression at the midline. PMID: 20381352
    3. Unilateral expression of Xnr-1 in the left lateral plate mesoderm directs the orientation of the left-right axis by driving the left-specific gene cascade. PMID: 16281170
    4. asymmetric expression of Nodal-related 1 (Xnr1) begins in the posterior left lateral plate mesoderm shortly after the initiation of bilateral perinotochordal expression in the posterior tailbud PMID: 16959238
    5. Xnr1 and derriere are coexpressed in the posterior paraxial mesoderm at neurula stage, and with Coco define a posttranscriptionally regulated signaling center in the chain leading to an increased TGF-beta signal on the left side of the embryo. PMID: 17239842

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 组织特异性：
    In the first phase of expression, localized to the vegetal region of the blastula. During gastrulation (stage 10.5), this expression disappears and instead becomes localized to the dorsal marginal zone, with enrichment in the organizer. During the second
  • 数据库链接：

    KEGG: xla:378543

    UniGene: Xl.1037