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  4. Recombinant Xenopus laevis Brachyury protein (t)

Recombinant Xenopus laevis Brachyury protein (t)

  • 货号：
    CSB-YP023052XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023052XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023052XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023052XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023052XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    t
  • Uniprot No.：
  • 别名：
    tbxt; bra; t; T-box transcription factor T; Brachyury protein; xBRA; Protein T
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-432
  • 氨基酸序列
    MSATESCAKN VQYRVDHLLS AVENELQAGS EKGDPTEKEL KVSLEERDLW TRFKELTNEM IVTKNGRRMF PVLKVSMSGL DPNAMYTVLL DFVAADNHRW KYVNGEWVPG GKPEPQAPSC VYIHPDSPNF GAHWMKDPVS FSKVKLTNKM NGGGQIMLNS LHKYEPRIHI VRVGGTQRMI TSHSFPETQF IAVTAYQNEE ITALKIKHNP FAKAFLDAKE RNDYKDILDE GIDSQHSNFS QLGTWLIPNG GSLCSPNPHT QFGAPLSLSS PHGCERYSSL RNHRSAPYPS PYTHRNNSPN NLADNSSACL SMLQSHDNWS TLQMPAHTGM LPMSHSTGTP PPSSQYPSLW SVSNSAITPV SQSGGITNGI SSQYLLGSTP HYSSLSHAVP SPSTGSPLYE HGAQTEIAEN QYDVTAHSRL SSTWTPVAPP SV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to the palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence. Causes dorsal mesodermal differentiation of animal cap ectoderm when co-expressed with wnt8 and noggin. None of these molecules causes dorsal mesoderm formation when expressed alone. Establishes the left/right axis at early gastrula stage by directly up-regulating mesodermal expression of zic3.
  • 基因功能参考文献：
    1. Disrupting the pattern of division by placing embryos in a temperature gradient resulted in highly asynchronous entry to the midblastula transition and misexpression of the mesodermal protein Xbra. PMID: 28978482
    2. DN-Xbra inhibits PV.1 expression and that the suppression of PV.1 reduces overall BMP signaling by inducing chordin and goosecoid expression. PMID: 24499677
    3. controls the early homeobox gene expression domain in the animal-vegetal direction PMID: 14729483
    4. findings reveal that the N-terminal domain that restricts the inducing activity of Xbra interacts with Smad1 PMID: 15809041
    5. Embryo elongation can occur through the synergistic effect(s) of the organizer molecule chordin, and the 'verall posteriorizing molecule Xbra. PMID: 18318733

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in presumptive mesodermal cells around the blastopore, and then in the notochord.
  • 数据库链接：

    KEGG: xla:399275

    UniGene: Xl.514